Amino acid dipepetide frequency for Massilia sp. NR 4-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.088AlaAla: 20.088 ± 0.275	1.24AlaCys: 1.24 ± 0.033	6.578AlaAsp: 6.578 ± 0.074	6.902AlaGlu: 6.902 ± 0.076	3.894AlaPhe: 3.894 ± 0.048	10.819AlaGly: 10.819 ± 0.135	2.381AlaHis: 2.381 ± 0.044	5.603AlaIle: 5.603 ± 0.066	4.116AlaLys: 4.116 ± 0.063	14.738AlaLeu: 14.738 ± 0.139	3.437AlaMet: 3.437 ± 0.049	3.432AlaAsn: 3.432 ± 0.062	6.394AlaPro: 6.394 ± 0.093	6.099AlaGln: 6.099 ± 0.095	8.203AlaArg: 8.203 ± 0.09	7.341AlaSer: 7.341 ± 0.086	5.592AlaThr: 5.592 ± 0.075	8.3AlaVal: 8.3 ± 0.081	1.64AlaTrp: 1.64 ± 0.035	2.971AlaTyr: 2.971 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.157CysAla: 1.157 ± 0.03	0.108CysCys: 0.108 ± 0.01	0.454CysAsp: 0.454 ± 0.019	0.473CysGlu: 0.473 ± 0.021	0.317CysPhe: 0.317 ± 0.014	0.867CysGly: 0.867 ± 0.025	0.245CysHis: 0.245 ± 0.014	0.399CysIle: 0.399 ± 0.014	0.255CysLys: 0.255 ± 0.013	0.852CysLeu: 0.852 ± 0.024	0.197CysMet: 0.197 ± 0.01	0.217CysAsn: 0.217 ± 0.012	0.387CysPro: 0.387 ± 0.016	0.29CysGln: 0.29 ± 0.017	0.541CysArg: 0.541 ± 0.021	0.548CysSer: 0.548 ± 0.021	0.388CysThr: 0.388 ± 0.016	0.57CysVal: 0.57 ± 0.019	0.134CysTrp: 0.134 ± 0.008	0.23CysTyr: 0.23 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.798AspAla: 6.798 ± 0.075	0.473AspCys: 0.473 ± 0.016	2.456AspAsp: 2.456 ± 0.039	3.025AspGlu: 3.025 ± 0.048	2.08AspPhe: 2.08 ± 0.042	4.883AspGly: 4.883 ± 0.066	0.939AspHis: 0.939 ± 0.027	2.563AspIle: 2.563 ± 0.04	2.048AspLys: 2.048 ± 0.04	5.034AspLeu: 5.034 ± 0.064	1.242AspMet: 1.242 ± 0.029	1.456AspAsn: 1.456 ± 0.032	2.571AspPro: 2.571 ± 0.04	1.789AspGln: 1.789 ± 0.039	2.671AspArg: 2.671 ± 0.042	2.77AspSer: 2.77 ± 0.042	2.445AspThr: 2.445 ± 0.038	3.528AspVal: 3.528 ± 0.055	0.839AspTrp: 0.839 ± 0.022	1.572AspTyr: 1.572 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.691GluAla: 6.691 ± 0.082	0.37GluCys: 0.37 ± 0.017	2.268GluAsp: 2.268 ± 0.037	3.111GluGlu: 3.111 ± 0.049	1.831GluPhe: 1.831 ± 0.033	3.59GluGly: 3.59 ± 0.051	1.311GluHis: 1.311 ± 0.031	2.721GluIle: 2.721 ± 0.047	2.253GluLys: 2.253 ± 0.049	6.421GluLeu: 6.421 ± 0.074	1.411GluMet: 1.411 ± 0.034	1.602GluAsn: 1.602 ± 0.033	2.004GluPro: 2.004 ± 0.038	3.224GluGln: 3.224 ± 0.045	4.527GluArg: 4.527 ± 0.067	2.538GluSer: 2.538 ± 0.04	2.502GluThr: 2.502 ± 0.039	3.682GluVal: 3.682 ± 0.055	0.734GluTrp: 0.734 ± 0.02	1.322GluTyr: 1.322 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.042PheAla: 4.042 ± 0.052	0.395PheCys: 0.395 ± 0.015	2.378PheAsp: 2.378 ± 0.037	1.829PheGlu: 1.829 ± 0.034	1.365PhePhe: 1.365 ± 0.03	3.392PheGly: 3.392 ± 0.054	0.72PheHis: 0.72 ± 0.021	1.591PheIle: 1.591 ± 0.035	1.269PheLys: 1.269 ± 0.028	3.135PheLeu: 3.135 ± 0.049	0.817PheMet: 0.817 ± 0.023	1.298PheAsn: 1.298 ± 0.031	1.453PhePro: 1.453 ± 0.031	1.183PheGln: 1.183 ± 0.026	1.869PheArg: 1.869 ± 0.032	2.5PheSer: 2.5 ± 0.04	1.922PheThr: 1.922 ± 0.037	2.419PheVal: 2.419 ± 0.036	0.492PheTrp: 0.492 ± 0.015	1.041PheTyr: 1.041 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	9.451GlyAla: 9.451 ± 0.099	0.767GlyCys: 0.767 ± 0.025	3.782GlyAsp: 3.782 ± 0.056	4.494GlyGlu: 4.494 ± 0.048	3.169GlyPhe: 3.169 ± 0.043	7.046GlyGly: 7.046 ± 0.11	1.79GlyHis: 1.79 ± 0.037	3.941GlyIle: 3.941 ± 0.051	3.995GlyLys: 3.995 ± 0.06	8.453GlyLeu: 8.453 ± 0.069	2.353GlyMet: 2.353 ± 0.043	2.648GlyAsn: 2.648 ± 0.06	2.615GlyPro: 2.615 ± 0.042	3.622GlyGln: 3.622 ± 0.05	5.064GlyArg: 5.064 ± 0.067	4.98GlySer: 4.98 ± 0.075	3.985GlyThr: 3.985 ± 0.064	5.946GlyVal: 5.946 ± 0.07	1.28GlyTrp: 1.28 ± 0.029	2.424GlyTyr: 2.424 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.643HisAla: 2.643 ± 0.047	0.266HisCys: 0.266 ± 0.012	1.053HisAsp: 1.053 ± 0.027	1.14HisGlu: 1.14 ± 0.029	0.917HisPhe: 0.917 ± 0.023	2.064HisGly: 2.064 ± 0.038	0.584HisHis: 0.584 ± 0.022	1.05HisIle: 1.05 ± 0.026	0.65HisLys: 0.65 ± 0.02	2.215HisLeu: 2.215 ± 0.038	0.512HisMet: 0.512 ± 0.017	0.58HisAsn: 0.58 ± 0.017	1.263HisPro: 1.263 ± 0.028	0.778HisGln: 0.778 ± 0.019	1.224HisArg: 1.224 ± 0.033	1.135HisSer: 1.135 ± 0.026	0.99HisThr: 0.99 ± 0.023	1.316HisVal: 1.316 ± 0.027	0.347HisTrp: 0.347 ± 0.015	0.686HisTyr: 0.686 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.138IleAla: 6.138 ± 0.072	0.434IleCys: 0.434 ± 0.016	3.102IleAsp: 3.102 ± 0.044	3.09IleGlu: 3.09 ± 0.047	1.426IlePhe: 1.426 ± 0.031	4.271IleGly: 4.271 ± 0.048	0.867IleHis: 0.867 ± 0.022	1.755IleIle: 1.755 ± 0.04	1.652IleLys: 1.652 ± 0.035	3.803IleLeu: 3.803 ± 0.058	0.86IleMet: 0.86 ± 0.026	1.531IleAsn: 1.531 ± 0.032	2.039IlePro: 2.039 ± 0.036	1.352IleGln: 1.352 ± 0.027	2.604IleArg: 2.604 ± 0.042	2.832IleSer: 2.832 ± 0.045	2.377IleThr: 2.377 ± 0.045	3.512IleVal: 3.512 ± 0.053	0.46IleTrp: 0.46 ± 0.017	1.114IleTyr: 1.114 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.41LysAla: 4.41 ± 0.062	0.171LysCys: 0.171 ± 0.01	1.822LysAsp: 1.822 ± 0.039	1.97LysGlu: 1.97 ± 0.033	1.079LysPhe: 1.079 ± 0.025	2.533LysGly: 2.533 ± 0.052	0.744LysHis: 0.744 ± 0.021	1.726LysIle: 1.726 ± 0.036	1.613LysLys: 1.613 ± 0.039	4.207LysLeu: 4.207 ± 0.059	0.973LysMet: 0.973 ± 0.022	1.228LysAsn: 1.228 ± 0.035	2.19LysPro: 2.19 ± 0.038	1.586LysGln: 1.586 ± 0.032	2.274LysArg: 2.274 ± 0.04	1.939LysSer: 1.939 ± 0.038	2.063LysThr: 2.063 ± 0.038	2.683LysVal: 2.683 ± 0.043	0.382LysTrp: 0.382 ± 0.017	0.848LysTyr: 0.848 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	15.392LeuAla: 15.392 ± 0.154	1.035LeuCys: 1.035 ± 0.028	5.953LeuAsp: 5.953 ± 0.071	5.598LeuGlu: 5.598 ± 0.07	3.561LeuPhe: 3.561 ± 0.05	8.314LeuGly: 8.314 ± 0.076	2.46LeuHis: 2.46 ± 0.045	4.357LeuIle: 4.357 ± 0.058	3.985LeuLys: 3.985 ± 0.05	12.35LeuLeu: 12.35 ± 0.148	2.393LeuMet: 2.393 ± 0.039	3.482LeuAsn: 3.482 ± 0.056	6.297LeuPro: 6.297 ± 0.082	4.59LeuGln: 4.59 ± 0.062	7.642LeuArg: 7.642 ± 0.091	7.032LeuSer: 7.032 ± 0.083	5.098LeuThr: 5.098 ± 0.062	6.757LeuVal: 6.757 ± 0.062	1.175LeuTrp: 1.175 ± 0.028	2.405LeuTyr: 2.405 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.02MetAla: 3.02 ± 0.046	0.155MetCys: 0.155 ± 0.009	1.18MetAsp: 1.18 ± 0.028	1.177MetGlu: 1.177 ± 0.031	0.701MetPhe: 0.701 ± 0.021	1.78MetGly: 1.78 ± 0.035	0.514MetHis: 0.514 ± 0.018	0.964MetIle: 0.964 ± 0.026	1.09MetLys: 1.09 ± 0.025	2.877MetLeu: 2.877 ± 0.045	0.623MetMet: 0.623 ± 0.02	0.933MetAsn: 0.933 ± 0.024	1.422MetPro: 1.422 ± 0.031	1.265MetGln: 1.265 ± 0.025	1.72MetArg: 1.72 ± 0.033	1.562MetSer: 1.562 ± 0.031	1.286MetThr: 1.286 ± 0.028	1.617MetVal: 1.617 ± 0.035	0.212MetTrp: 0.212 ± 0.011	0.487MetTyr: 0.487 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.704AsnAla: 3.704 ± 0.053	0.263AsnCys: 0.263 ± 0.013	1.483AsnAsp: 1.483 ± 0.035	1.462AsnGlu: 1.462 ± 0.032	1.11AsnPhe: 1.11 ± 0.025	2.87AsnGly: 2.87 ± 0.057	0.571AsnHis: 0.571 ± 0.02	1.534AsnIle: 1.534 ± 0.037	1.083AsnLys: 1.083 ± 0.031	3.129AsnLeu: 3.129 ± 0.041	0.735AsnMet: 0.735 ± 0.021	1.035AsnAsn: 1.035 ± 0.029	1.868AsnPro: 1.868 ± 0.035	1.123AsnGln: 1.123 ± 0.027	1.774AsnArg: 1.774 ± 0.041	1.657AsnSer: 1.657 ± 0.037	1.673AsnThr: 1.673 ± 0.033	2.154AsnVal: 2.154 ± 0.043	0.484AsnTrp: 0.484 ± 0.018	0.95AsnTyr: 0.95 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.249ProAla: 7.249 ± 0.108	0.317ProCys: 0.317 ± 0.014	2.828ProAsp: 2.828 ± 0.038	3.177ProGlu: 3.177 ± 0.045	1.676ProPhe: 1.676 ± 0.03	4.181ProGly: 4.181 ± 0.049	1.017ProHis: 1.017 ± 0.03	1.755ProIle: 1.755 ± 0.034	1.494ProLys: 1.494 ± 0.034	5.292ProLeu: 5.292 ± 0.065	1.085ProMet: 1.085 ± 0.026	1.383ProAsn: 1.383 ± 0.028	2.57ProPro: 2.57 ± 0.052	2.322ProGln: 2.322 ± 0.038	2.548ProArg: 2.548 ± 0.047	2.59ProSer: 2.59 ± 0.038	1.98ProThr: 1.98 ± 0.036	3.791ProVal: 3.791 ± 0.051	0.612ProTrp: 0.612 ± 0.02	1.152ProTyr: 1.152 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	6.127GlnAla: 6.127 ± 0.079	0.274GlnCys: 0.274 ± 0.013	1.851GlnAsp: 1.851 ± 0.033	2.08GlnGlu: 2.08 ± 0.036	1.454GlnPhe: 1.454 ± 0.031	3.159GlnGly: 3.159 ± 0.048	1.093GlnHis: 1.093 ± 0.025	2.068GlnIle: 2.068 ± 0.059	1.349GlnLys: 1.349 ± 0.029	5.019GlnLeu: 5.019 ± 0.072	1.106GlnMet: 1.106 ± 0.027	1.182GlnAsn: 1.182 ± 0.029	2.287GlnPro: 2.287 ± 0.042	2.5GlnGln: 2.5 ± 0.051	3.58GlnArg: 3.58 ± 0.051	2.174GlnSer: 2.174 ± 0.037	2.012GlnThr: 2.012 ± 0.036	2.935GlnVal: 2.935 ± 0.041	0.595GlnTrp: 0.595 ± 0.021	0.958GlnTyr: 0.958 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.143ArgAla: 7.143 ± 0.077	0.531ArgCys: 0.531 ± 0.021	3.247ArgAsp: 3.247 ± 0.045	3.828ArgGlu: 3.828 ± 0.047	2.556ArgPhe: 2.556 ± 0.042	4.156ArgGly: 4.156 ± 0.054	1.783ArgHis: 1.783 ± 0.035	3.395ArgIle: 3.395 ± 0.055	2.332ArgLys: 2.332 ± 0.044	7.502ArgLeu: 7.502 ± 0.091	1.763ArgMet: 1.763 ± 0.038	2.049ArgAsn: 2.049 ± 0.036	2.856ArgPro: 2.856 ± 0.047	3.292ArgGln: 3.292 ± 0.048	4.572ArgArg: 4.572 ± 0.066	3.598ArgSer: 3.598 ± 0.047	2.739ArgThr: 2.739 ± 0.044	4.141ArgVal: 4.141 ± 0.056	0.96ArgTrp: 0.96 ± 0.026	1.965ArgTyr: 1.965 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.195SerAla: 7.195 ± 0.093	0.456SerCys: 0.456 ± 0.015	2.925SerAsp: 2.925 ± 0.043	2.815SerGlu: 2.815 ± 0.043	2.327SerPhe: 2.327 ± 0.04	5.738SerGly: 5.738 ± 0.095	1.272SerHis: 1.272 ± 0.024	2.705SerIle: 2.705 ± 0.045	2.036SerLys: 2.036 ± 0.04	6.147SerLeu: 6.147 ± 0.067	1.409SerMet: 1.409 ± 0.031	1.781SerAsn: 1.781 ± 0.044	2.462SerPro: 2.462 ± 0.039	2.154SerGln: 2.154 ± 0.034	3.327SerArg: 3.327 ± 0.049	3.583SerSer: 3.583 ± 0.059	2.908SerThr: 2.908 ± 0.062	4.042SerVal: 4.042 ± 0.051	0.819SerTrp: 0.819 ± 0.024	1.79SerTyr: 1.79 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	5.908ThrAla: 5.908 ± 0.085	0.326ThrCys: 0.326 ± 0.014	2.176ThrAsp: 2.176 ± 0.04	2.258ThrGlu: 2.258 ± 0.038	1.605ThrPhe: 1.605 ± 0.034	4.111ThrGly: 4.111 ± 0.062	0.905ThrHis: 0.905 ± 0.023	2.361ThrIle: 2.361 ± 0.042	1.305ThrLys: 1.305 ± 0.03	6.064ThrLeu: 6.064 ± 0.069	1.083ThrMet: 1.083 ± 0.027	1.257ThrAsn: 1.257 ± 0.032	3.085ThrPro: 3.085 ± 0.041	1.785ThrGln: 1.785 ± 0.038	2.84ThrArg: 2.84 ± 0.049	2.633ThrSer: 2.633 ± 0.048	2.435ThrThr: 2.435 ± 0.054	4.118ThrVal: 4.118 ± 0.066	0.533ThrTrp: 0.533 ± 0.02	1.208ThrTyr: 1.208 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	8.564ValAla: 8.564 ± 0.083	0.65ValCys: 0.65 ± 0.023	3.483ValAsp: 3.483 ± 0.053	3.807ValGlu: 3.807 ± 0.058	2.495ValPhe: 2.495 ± 0.043	4.999ValGly: 4.999 ± 0.07	1.387ValHis: 1.387 ± 0.025	3.159ValIle: 3.159 ± 0.048	2.562ValLys: 2.562 ± 0.041	7.987ValLeu: 7.987 ± 0.08	1.662ValMet: 1.662 ± 0.032	2.296ValAsn: 2.296 ± 0.04	3.38ValPro: 3.38 ± 0.056	2.879ValGln: 2.879 ± 0.041	4.393ValArg: 4.393 ± 0.053	4.147ValSer: 4.147 ± 0.059	3.594ValThr: 3.594 ± 0.064	5.066ValVal: 5.066 ± 0.068	0.839ValTrp: 0.839 ± 0.022	1.689ValTyr: 1.689 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.04TrpAla: 1.04 ± 0.023	0.131TrpCys: 0.131 ± 0.009	0.59TrpAsp: 0.59 ± 0.022	0.563TrpGlu: 0.563 ± 0.019	0.494TrpPhe: 0.494 ± 0.018	0.817TrpGly: 0.817 ± 0.026	0.335TrpHis: 0.335 ± 0.014	0.599TrpIle: 0.599 ± 0.019	0.453TrpLys: 0.453 ± 0.018	1.935TrpLeu: 1.935 ± 0.039	0.394TrpMet: 0.394 ± 0.013	0.481TrpAsn: 0.481 ± 0.018	0.611TrpPro: 0.611 ± 0.022	0.845TrpGln: 0.845 ± 0.022	1.111TrpArg: 1.111 ± 0.028	0.788TrpSer: 0.788 ± 0.026	0.646TrpThr: 0.646 ± 0.023	0.794TrpVal: 0.794 ± 0.02	0.222TrpTrp: 0.222 ± 0.012	0.311TrpTyr: 0.311 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.007TyrAla: 3.007 ± 0.051	0.268TyrCys: 0.268 ± 0.014	1.574TyrAsp: 1.574 ± 0.081	1.316TyrGlu: 1.316 ± 0.026	1.055TyrPhe: 1.055 ± 0.024	2.215TyrGly: 2.215 ± 0.037	0.493TyrHis: 0.493 ± 0.015	0.972TyrIle: 0.972 ± 0.025	0.899TyrLys: 0.899 ± 0.025	2.717TyrLeu: 2.717 ± 0.045	0.531TyrMet: 0.531 ± 0.015	0.792TyrAsn: 0.792 ± 0.03	1.269TyrPro: 1.269 ± 0.028	1.123TyrGln: 1.123 ± 0.026	1.923TyrArg: 1.923 ± 0.038	1.544TyrSer: 1.544 ± 0.033	1.358TyrThr: 1.358 ± 0.034	1.668TyrVal: 1.668 ± 0.03	0.401TyrTrp: 0.401 ± 0.02	0.753TyrTyr: 0.753 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5022 proteins (1773294 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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