Beihai picobirna-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLI7|A0A1L3KLI7_9VIRU Uncharacterized protein OS=Beihai picobirna-like virus 8 OX=1922525 PE=4 SV=1
MM1 pKa = 7.24VKK3 pKa = 10.3HH4 pKa = 5.76NQFSFLDD11 pKa = 3.78SLDD14 pKa = 3.43LDD16 pKa = 4.04KK17 pKa = 11.07EE18 pKa = 4.5VKK20 pKa = 10.16LRR22 pKa = 11.84LSSNLLNIIQGNDD35 pKa = 3.03TVLYY39 pKa = 9.24SPLVKK44 pKa = 10.13SVKK47 pKa = 9.86PEE49 pKa = 4.34SILTGWDD56 pKa = 3.48KK57 pKa = 10.93IFKK60 pKa = 9.93SKK62 pKa = 9.71SVKK65 pKa = 8.95MNKK68 pKa = 8.98VLHH71 pKa = 6.65DD72 pKa = 4.41LEE74 pKa = 6.56DD75 pKa = 4.64DD76 pKa = 3.63NRR78 pKa = 11.84SKK80 pKa = 11.19YY81 pKa = 10.23GPRR84 pKa = 11.84SIAVPWVDD92 pKa = 3.12RR93 pKa = 11.84RR94 pKa = 11.84SDD96 pKa = 3.4VLSYY100 pKa = 11.0FEE102 pKa = 4.64SNNMEE107 pKa = 4.41RR108 pKa = 11.84PPLGLPSLRR117 pKa = 11.84RR118 pKa = 11.84NLRR121 pKa = 11.84PLEE124 pKa = 3.98LDD126 pKa = 3.51KK127 pKa = 11.26AIKK130 pKa = 9.98LLKK133 pKa = 10.28NDD135 pKa = 4.03TNSGLPYY142 pKa = 9.83YY143 pKa = 9.95RR144 pKa = 11.84RR145 pKa = 11.84KK146 pKa = 10.71GGLKK150 pKa = 10.21DD151 pKa = 3.24IYY153 pKa = 10.89LKK155 pKa = 10.78EE156 pKa = 4.03FTNILPRR163 pKa = 11.84EE164 pKa = 4.51DD165 pKa = 3.59PCVMFTRR172 pKa = 11.84TQEE175 pKa = 4.04GKK177 pKa = 8.45KK178 pKa = 8.45TRR180 pKa = 11.84TVWGYY185 pKa = 10.41PMADD189 pKa = 3.6TVNEE193 pKa = 4.09MCVYY197 pKa = 10.56SPLLGYY203 pKa = 7.94QRR205 pKa = 11.84KK206 pKa = 9.51LNWRR210 pKa = 11.84SCLNSPDD217 pKa = 3.52AVNIEE222 pKa = 3.88ITKK225 pKa = 10.86LIDD228 pKa = 3.2KK229 pKa = 10.2ALKK232 pKa = 10.02QDD234 pKa = 3.42VSLLSIDD241 pKa = 4.28FSAYY245 pKa = 9.93DD246 pKa = 3.44ASLDD250 pKa = 4.24RR251 pKa = 11.84PLQKK255 pKa = 10.7AAFEE259 pKa = 4.56YY260 pKa = 10.17ISKK263 pKa = 10.18LFQPSYY269 pKa = 11.39EE270 pKa = 3.96EE271 pKa = 5.24LINNLFNRR279 pKa = 11.84FNTIGLVTPDD289 pKa = 4.68GILSGHH295 pKa = 6.68HH296 pKa = 5.99GVPSGSTFTNEE307 pKa = 3.13VDD309 pKa = 5.39SIIQYY314 pKa = 10.63LIALDD319 pKa = 4.05YY320 pKa = 11.05GLEE323 pKa = 4.24DD324 pKa = 4.27SSMQIQGDD332 pKa = 3.76DD333 pKa = 3.18GVYY336 pKa = 10.17CVQDD340 pKa = 3.42GTALAEE346 pKa = 4.15HH347 pKa = 6.7FKK349 pKa = 11.35SFGLNVNFEE358 pKa = 3.81KK359 pKa = 10.49SYY361 pKa = 10.61YY362 pKa = 9.85SKK364 pKa = 10.77DD365 pKa = 3.39YY366 pKa = 10.93CVYY369 pKa = 10.62LQNLYY374 pKa = 10.88HH375 pKa = 6.81NDD377 pKa = 3.34YY378 pKa = 9.97RR379 pKa = 11.84DD380 pKa = 3.55SSGKK384 pKa = 9.56ICGIYY389 pKa = 7.99PTYY392 pKa = 10.42RR393 pKa = 11.84ALNRR397 pKa = 11.84LIHH400 pKa = 6.14PEE402 pKa = 3.91RR403 pKa = 11.84FVDD406 pKa = 5.13FEE408 pKa = 5.14DD409 pKa = 3.54IKK411 pKa = 11.13ISGRR415 pKa = 11.84DD416 pKa = 3.43YY417 pKa = 10.78FSIRR421 pKa = 11.84SICILEE427 pKa = 3.92NCKK430 pKa = 10.1YY431 pKa = 10.5HH432 pKa = 7.29PLFEE436 pKa = 5.56DD437 pKa = 3.82LVKK440 pKa = 10.91YY441 pKa = 10.37IYY443 pKa = 10.94KK444 pKa = 10.01LDD446 pKa = 3.67KK447 pKa = 11.24YY448 pKa = 9.82NLKK451 pKa = 10.44FSRR454 pKa = 11.84DD455 pKa = 3.26SLFRR459 pKa = 11.84FVQNRR464 pKa = 11.84KK465 pKa = 8.92QEE467 pKa = 4.18SGVQGIFNYY476 pKa = 10.18RR477 pKa = 11.84YY478 pKa = 8.66EE479 pKa = 5.33DD480 pKa = 3.97DD481 pKa = 3.65VSGIDD486 pKa = 3.24SFEE489 pKa = 4.12TMKK492 pKa = 11.0VLNKK496 pKa = 10.39LL497 pKa = 3.46

Molecular weight: 57.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLI7|A0A1L3KLI7_9VIRU Uncharacterized protein OS=Beihai picobirna-like virus 8 OX=1922525 PE=4 SV=1
MM1 pKa = 6.37YY2 pKa = 8.89TSNFIYY8 pKa = 9.87LKK10 pKa = 10.83HH11 pKa = 6.25MNNEE15 pKa = 3.75YY16 pKa = 10.61KK17 pKa = 10.7KK18 pKa = 10.63PFFRR22 pKa = 11.84KK23 pKa = 9.87NKK25 pKa = 7.13TDD27 pKa = 3.43KK28 pKa = 11.43NIDD31 pKa = 3.44SDD33 pKa = 4.45SSVSTDD39 pKa = 2.71STQRR43 pKa = 11.84NEE45 pKa = 4.45DD46 pKa = 3.68STSSLQASGNPAFVPGGARR65 pKa = 11.84PWGVTGSQSIPPMGVSRR82 pKa = 11.84NVYY85 pKa = 8.53RR86 pKa = 11.84QINGGVARR94 pKa = 11.84ALGSDD99 pKa = 2.82IGKK102 pKa = 10.16AVIGEE107 pKa = 4.16VASYY111 pKa = 11.46GLTKK115 pKa = 10.78LNSKK119 pKa = 10.75LNDD122 pKa = 3.49FEE124 pKa = 6.32SEE126 pKa = 4.2FEE128 pKa = 4.05NRR130 pKa = 11.84KK131 pKa = 8.76PPRR134 pKa = 11.84NKK136 pKa = 10.44GKK138 pKa = 8.6GTRR141 pKa = 11.84KK142 pKa = 9.65RR143 pKa = 11.84GRR145 pKa = 11.84GNRR148 pKa = 11.84DD149 pKa = 2.98SGSGNGGGGSDD160 pKa = 3.66NNYY163 pKa = 10.24SGNSSGEE170 pKa = 3.62RR171 pKa = 11.84GVPYY175 pKa = 10.17GGSPMPYY182 pKa = 8.19RR183 pKa = 11.84NRR185 pKa = 11.84YY186 pKa = 8.78ILDD189 pKa = 3.48TGMSASTIIDD199 pKa = 3.94DD200 pKa = 4.64DD201 pKa = 4.88QNTTDD206 pKa = 3.37HH207 pKa = 7.07WSPLLTTNGSLLDD220 pKa = 3.96ILWVPQTRR228 pKa = 11.84TYY230 pKa = 11.44DD231 pKa = 3.47VIRR234 pKa = 11.84TYY236 pKa = 11.37VFDD239 pKa = 4.54KK240 pKa = 11.31YY241 pKa = 11.06LDD243 pKa = 3.89KK244 pKa = 11.06LQQDD248 pKa = 4.77LNPEE252 pKa = 4.03YY253 pKa = 10.7TIDD256 pKa = 3.47TDD258 pKa = 3.93VFGSYY263 pKa = 10.66LSDD266 pKa = 3.19VSEE269 pKa = 4.51ALQAYY274 pKa = 6.94YY275 pKa = 10.49TLGHH279 pKa = 5.99IRR281 pKa = 11.84AFAFGPYY288 pKa = 8.87RR289 pKa = 11.84QQNIALYY296 pKa = 10.1KK297 pKa = 8.96IGRR300 pKa = 11.84RR301 pKa = 11.84YY302 pKa = 10.47GSDD305 pKa = 3.17VIDD308 pKa = 3.15AHH310 pKa = 7.12RR311 pKa = 11.84YY312 pKa = 8.75LKK314 pKa = 10.81SKK316 pKa = 10.92LEE318 pKa = 4.03NLPIPPRR325 pKa = 11.84LVHH328 pKa = 6.07FMYY331 pKa = 10.88YY332 pKa = 8.68FTQNFTMHH340 pKa = 6.95PTPASPCIRR349 pKa = 11.84INYY352 pKa = 9.62RR353 pKa = 11.84GMFLPGKK360 pKa = 7.97NRR362 pKa = 11.84KK363 pKa = 7.88WKK365 pKa = 10.34DD366 pKa = 3.25YY367 pKa = 10.73GVNCSISADD376 pKa = 3.79LFKK379 pKa = 11.39GLIDD383 pKa = 3.97NLDD386 pKa = 3.6KK387 pKa = 11.28HH388 pKa = 6.61RR389 pKa = 11.84LTYY392 pKa = 11.04NKK394 pKa = 9.51INRR397 pKa = 11.84ICPEE401 pKa = 4.18LKK403 pKa = 10.15VGQLMDD409 pKa = 4.51PSCEE413 pKa = 4.12VIYY416 pKa = 10.56DD417 pKa = 3.82EE418 pKa = 6.01QFNTWWHH425 pKa = 4.95NHH427 pKa = 4.85CTSYY431 pKa = 10.61WEE433 pKa = 4.57GGKK436 pKa = 8.08LTYY439 pKa = 9.08TRR441 pKa = 11.84NVADD445 pKa = 4.4LRR447 pKa = 11.84LTAYY451 pKa = 10.07YY452 pKa = 10.15GQYY455 pKa = 9.91TNRR458 pKa = 11.84LDD460 pKa = 3.56TAIYY464 pKa = 10.11ASSDD468 pKa = 3.41LAVRR472 pKa = 11.84NAEE475 pKa = 3.78GDD477 pKa = 3.7YY478 pKa = 11.26YY479 pKa = 10.3MYY481 pKa = 10.57PGVWCPFNGWKK492 pKa = 8.27NTDD495 pKa = 3.19AVKK498 pKa = 10.89DD499 pKa = 4.01EE500 pKa = 4.89GPLTSINYY508 pKa = 7.79WRR510 pKa = 11.84EE511 pKa = 3.6DD512 pKa = 2.88KK513 pKa = 10.51IYY515 pKa = 10.74NMNYY519 pKa = 8.91TSSKK523 pKa = 10.92EE524 pKa = 3.98NVKK527 pKa = 9.64WGPFSNVYY535 pKa = 9.65KK536 pKa = 10.96VPLIDD541 pKa = 4.39DD542 pKa = 3.88HH543 pKa = 6.46EE544 pKa = 4.59KK545 pKa = 10.4KK546 pKa = 10.52IKK548 pKa = 10.37IFTGSDD554 pKa = 3.17CGVQIAQEE562 pKa = 4.02HH563 pKa = 5.76NAALMGDD570 pKa = 3.72QAKK573 pKa = 10.65KK574 pKa = 9.43LISWIFEE581 pKa = 4.07TT582 pKa = 5.44

Molecular weight: 66.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1079

497

582

539.5

61.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.707 ± 0.738

1.39 ± 0.148

7.507 ± 0.366

4.263 ± 0.518

4.078 ± 0.508

7.044 ± 1.225

1.761 ± 0.102

5.931 ± 0.207

6.951 ± 0.47

8.897 ± 1.738

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.854 ± 0.165

6.951 ± 0.482

4.634 ± 0.276

3.058 ± 0.027

5.653 ± 0.013

8.341 ± 0.346

4.912 ± 1.008

5.19 ± 0.436

1.483 ± 0.458

6.395 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski