Sandaracinobacter sp. PAMC 28131
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3327 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A501XNG0|A0A501XNG0_9SPHN Cysteine hydrolase OS=Sandaracinobacter sp. PAMC 28131 OX=1715348 GN=FJQ54_06625 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 ILTGLLLGCASFVALSSTPASAATCFGNCGEE33 pKa = 4.3 TNINGGQTGSVGVPPLQGDD52 pKa = 3.64 NVYY55 pKa = 10.95 YY56 pKa = 9.94 WVSTNGGSTGGGTLPGQEE74 pKa = 4.19 SGSTNGSRR82 pKa = 11.84 LVSDD86 pKa = 4.07 SFYY89 pKa = 11.18 AAAGQKK95 pKa = 9.47 VSYY98 pKa = 8.57 YY99 pKa = 10.46 FNYY102 pKa = 8.15 ITSDD106 pKa = 3.14 GAGYY110 pKa = 11.0 ADD112 pKa = 4.27 YY113 pKa = 10.89 SWSQLRR119 pKa = 11.84 DD120 pKa = 3.46 VNDD123 pKa = 3.54 SSNVTNLFTARR134 pKa = 11.84 TKK136 pKa = 10.63 PSGTIVPGLDD146 pKa = 3.55 LPDD149 pKa = 3.81 VEE151 pKa = 5.15 ATLSPATVLIKK162 pKa = 10.6 PGTTFAPLGDD172 pKa = 3.68 SSGACWSAGCGNTGWIYY189 pKa = 11.07 SEE191 pKa = 4.12 YY192 pKa = 10.04 TIQTSGTYY200 pKa = 9.65 EE201 pKa = 3.95 LVFGVSNWDD210 pKa = 3.16 DD211 pKa = 3.56 TAFDD215 pKa = 3.54 SALAFDD221 pKa = 4.62 GLLIGGIVIGDD232 pKa = 3.97 GSSPTNPLAPTDD244 pKa = 4.12 LQPDD248 pKa = 4.12 GSFVFSFEE256 pKa = 4.05 VTQPEE261 pKa = 4.2 QPVFIDD267 pKa = 3.98 PYY269 pKa = 8.7 VAVGYY274 pKa = 9.49 EE275 pKa = 3.92 YY276 pKa = 10.9 EE277 pKa = 4.44 VIDD280 pKa = 4.19 GSLAFSQAWFGDD292 pKa = 3.31 VGDD295 pKa = 4.37 PDD297 pKa = 5.65 GYY299 pKa = 10.75 QIWGWDD305 pKa = 3.2 GADD308 pKa = 3.49 YY309 pKa = 11.47 YY310 pKa = 11.43 FLTDD314 pKa = 3.32 ITAEE318 pKa = 3.65 TWYY321 pKa = 10.83 AFASPVTKK329 pKa = 10.65 FKK331 pKa = 11.24 LLGIDD336 pKa = 3.69 SDD338 pKa = 4.59 LGLDD342 pKa = 3.95 PEE344 pKa = 4.98 DD345 pKa = 4.14 PNAFVSGFIYY355 pKa = 10.73 NGTGKK360 pKa = 10.1 VDD362 pKa = 3.63 VSMKK366 pKa = 10.5 PITEE370 pKa = 4.02 WVEE373 pKa = 3.92 GGGGGVVPEE382 pKa = 4.08 PATWAMMILGFGAVGLAARR401 pKa = 11.84 RR402 pKa = 11.84 RR403 pKa = 11.84 RR404 pKa = 11.84 SHH406 pKa = 6.59 SVTAA410 pKa = 4.29
Molecular weight: 43.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 0.324
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|A0A501XDP2|A0A501XDP2_9SPHN 3'(2') 5'-bisphosphate nucleotidase CysQ OS=Sandaracinobacter sp. PAMC 28131 OX=1715348 GN=cysQ PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.39 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTVGGRR28 pKa = 11.84 KK29 pKa = 8.56 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.11
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3327
0
3327
1063820
37
3595
319.8
34.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.694 ± 0.061
0.674 ± 0.011
5.574 ± 0.029
5.369 ± 0.045
3.505 ± 0.025
9.058 ± 0.083
1.938 ± 0.02
4.729 ± 0.027
2.939 ± 0.033
10.529 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.328 ± 0.022
2.477 ± 0.031
5.698 ± 0.04
3.218 ± 0.026
7.299 ± 0.053
5.335 ± 0.038
5.142 ± 0.039
7.013 ± 0.039
1.484 ± 0.019
1.999 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here