Eragrostis curvula (weeping love grass)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Chloridoideae; Eragrostideae; Eragrostidinae; Eragrostis

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J9SGR1|A0A5J9SGR1_9POAL Uncharacterized protein (Fragment) OS=Eragrostis curvula OX=38414 GN=EJB05_56692 PE=4 SV=1
MM1 pKa = 7.41ATTVHH6 pKa = 6.18PQPPQFAPLPRR17 pKa = 11.84PPRR20 pKa = 11.84ADD22 pKa = 3.34DD23 pKa = 4.93AVALDD28 pKa = 5.11DD29 pKa = 5.53DD30 pKa = 5.55DD31 pKa = 6.87SDD33 pKa = 4.54GEE35 pKa = 4.71SVAEE39 pKa = 4.13SCPWPHH45 pKa = 6.1QRR47 pKa = 11.84GAAAAEE53 pKa = 4.5SSCGSSTCCHH63 pKa = 6.27GEE65 pKa = 4.31EE66 pKa = 5.02MDD68 pKa = 6.25DD69 pKa = 5.88DD70 pKa = 6.39DD71 pKa = 7.61DD72 pKa = 7.28DD73 pKa = 5.67DD74 pKa = 4.95GCSSCVEE81 pKa = 4.03GDD83 pKa = 3.12EE84 pKa = 4.29CSYY87 pKa = 10.79YY88 pKa = 10.48QYY90 pKa = 10.97EE91 pKa = 4.23LQEE94 pKa = 4.41AADD97 pKa = 4.9DD98 pKa = 4.32DD99 pKa = 5.51DD100 pKa = 4.58EE101 pKa = 4.97VGNAAAGSGGVWWEE115 pKa = 3.93RR116 pKa = 11.84MAVARR121 pKa = 11.84NNAGGAFPPPPPEE134 pKa = 4.79PEE136 pKa = 4.06DD137 pKa = 3.37PKK139 pKa = 10.84RR140 pKa = 11.84AAEE143 pKa = 4.0RR144 pKa = 11.84QEE146 pKa = 3.79EE147 pKa = 4.08DD148 pKa = 3.22RR149 pKa = 11.84KK150 pKa = 10.52FWEE153 pKa = 4.84DD154 pKa = 3.63CLATGYY160 pKa = 9.69PP161 pKa = 3.7

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J9VNR7|A0A5J9VNR7_9POAL Uncharacterized protein (Fragment) OS=Eragrostis curvula OX=38414 GN=EJB05_10763 PE=4 SV=1
MM1 pKa = 7.4AASLVHH7 pKa = 6.73AVAAPLPSAGRR18 pKa = 11.84PARR21 pKa = 11.84AFHH24 pKa = 6.84PLASAPALASRR35 pKa = 11.84APRR38 pKa = 11.84PRR40 pKa = 11.84PVAASSFRR48 pKa = 11.84SRR50 pKa = 11.84PSRR53 pKa = 11.84RR54 pKa = 11.84LVPGVSSEE62 pKa = 3.89GARR65 pKa = 11.84PVGVASSPPSRR76 pKa = 11.84GRR78 pKa = 11.84RR79 pKa = 11.84GVQGWKK85 pKa = 8.39MARR88 pKa = 11.84TTSMWRR94 pKa = 11.84FSLRR98 pKa = 11.84RR99 pKa = 11.84LRR101 pKa = 11.84KK102 pKa = 9.55LGRR105 pKa = 3.57

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53658

0

53658

20519742

8

5164

382.4

42.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.09 ± 0.014

2.025 ± 0.005

5.459 ± 0.007

5.944 ± 0.011

3.731 ± 0.006

7.068 ± 0.01

2.499 ± 0.004

4.371 ± 0.006

4.902 ± 0.011

9.623 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.004

3.483 ± 0.008

5.679 ± 0.01

3.415 ± 0.008

6.429 ± 0.009

8.255 ± 0.011

4.851 ± 0.006

6.857 ± 0.008

1.345 ± 0.004

2.552 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski