Diadromus pulchellus ascovirus 4a
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2NZ07|F2NZ07_9VIRU Uncharacterized protein OS=Diadromus pulchellus ascovirus 4a OX=158683 PE=4 SV=1
MM1 pKa = 7.37 FPVCSLCQSDD11 pKa = 3.78 EE12 pKa = 4.33 FVVEE16 pKa = 3.45 KK17 pKa = 10.46 HH18 pKa = 4.7 YY19 pKa = 9.91 TLFEE23 pKa = 4.43 NGEE26 pKa = 4.41 VFVSDD31 pKa = 4.13 DD32 pKa = 3.32 TTVYY36 pKa = 9.27 CTRR39 pKa = 11.84 CRR41 pKa = 11.84 DD42 pKa = 3.38 VCAMCSKK49 pKa = 10.51 IEE51 pKa = 3.86 VRR53 pKa = 11.84 NKK55 pKa = 9.91 ISVGAHH61 pKa = 5.48 FPNANVSATFKK72 pKa = 9.91 MCKK75 pKa = 9.21 EE76 pKa = 4.38 CVRR79 pKa = 11.84 SCDD82 pKa = 3.49 SCVDD86 pKa = 3.09 CHH88 pKa = 6.37 TRR90 pKa = 11.84 FDD92 pKa = 3.38 HH93 pKa = 8.05 DD94 pKa = 4.65 YY95 pKa = 9.62 IRR97 pKa = 11.84 RR98 pKa = 11.84 WKK100 pKa = 10.61 DD101 pKa = 2.84 AGRR104 pKa = 11.84 DD105 pKa = 3.72 DD106 pKa = 5.83 DD107 pKa = 6.0 LLIPVNFYY115 pKa = 10.92 LVDD118 pKa = 3.99 RR119 pKa = 11.84 NLVSLCYY126 pKa = 9.44 TCVPNAVCRR135 pKa = 11.84 FGHH138 pKa = 5.42 YY139 pKa = 9.33 TLGDD143 pKa = 3.85 CFEE146 pKa = 4.82 CGLWEE151 pKa = 4.09 EE152 pKa = 4.64 EE153 pKa = 3.75 QDD155 pKa = 4.25 SGYY158 pKa = 10.62 EE159 pKa = 3.77 SCYY162 pKa = 11.07 DD163 pKa = 3.53 SDD165 pKa = 4.11 EE166 pKa = 5.17 SDD168 pKa = 3.66 DD169 pKa = 5.44 AEE171 pKa = 4.77 DD172 pKa = 3.99 EE173 pKa = 4.43 SAEE176 pKa = 4.25 PEE178 pKa = 4.17 DD179 pKa = 3.79 PFYY182 pKa = 11.46 DD183 pKa = 4.21 EE184 pKa = 5.51 MFEE187 pKa = 4.39 TDD189 pKa = 5.91 DD190 pKa = 3.77 NTEE193 pKa = 4.56 DD194 pKa = 4.68 DD195 pKa = 4.3 DD196 pKa = 4.43
Molecular weight: 22.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 4.012
IPC_protein 4.012
Toseland 3.795
ProMoST 4.126
Dawson 3.986
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.935
Nozaki 4.101
DTASelect 4.329
Thurlkill 3.834
EMBOSS 3.923
Sillero 4.126
Patrickios 0.388
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.034
Protein with the highest isoelectric point:
>tr|F2NYV1|F2NYV1_9VIRU HMG box domain-containing protein OS=Diadromus pulchellus ascovirus 4a OX=158683 PE=4 SV=1
MM1 pKa = 7.04 DD2 pKa = 4.6 TKK4 pKa = 10.67 RR5 pKa = 11.84 KK6 pKa = 9.08 RR7 pKa = 11.84 APRR10 pKa = 11.84 NSAGLGTEE18 pKa = 3.58 WTARR22 pKa = 11.84 SGRR25 pKa = 11.84 AAMASTTRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SPSPRR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 SATRR46 pKa = 11.84 ASPAAVSTRR55 pKa = 11.84 RR56 pKa = 11.84 SPSRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 SRR64 pKa = 11.84 AASPTRR70 pKa = 11.84 RR71 pKa = 11.84 ARR73 pKa = 11.84 RR74 pKa = 11.84 SPVVTPALVVTASQTPVRR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 RR96 pKa = 11.84 SAARR100 pKa = 11.84 SPSPARR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 SATRR113 pKa = 11.84 RR114 pKa = 11.84 SPSPRR119 pKa = 11.84 RR120 pKa = 11.84 ASPARR125 pKa = 11.84 RR126 pKa = 11.84 RR127 pKa = 11.84 RR128 pKa = 11.84 SAARR132 pKa = 11.84 SPSPRR137 pKa = 11.84 RR138 pKa = 11.84 KK139 pKa = 9.27 PATSPARR146 pKa = 11.84 RR147 pKa = 11.84 RR148 pKa = 11.84 RR149 pKa = 11.84 SATRR153 pKa = 11.84 RR154 pKa = 11.84 SPSPARR160 pKa = 11.84 STSKK164 pKa = 10.23 RR165 pKa = 11.84 RR166 pKa = 11.84 SSPRR170 pKa = 11.84 KK171 pKa = 7.99 PRR173 pKa = 11.84 RR174 pKa = 11.84 SPRR177 pKa = 11.84 RR178 pKa = 11.84 AKK180 pKa = 10.65 SPAAKK185 pKa = 9.36 PRR187 pKa = 11.84 RR188 pKa = 11.84 RR189 pKa = 11.84 RR190 pKa = 11.84 RR191 pKa = 11.84 SRR193 pKa = 11.84 KK194 pKa = 9.16 SRR196 pKa = 11.84 ASSTDD201 pKa = 2.55 EE202 pKa = 4.44 FMARR206 pKa = 11.84 DD207 pKa = 3.73 HH208 pKa = 6.63 KK209 pKa = 11.08 FSLFDD214 pKa = 3.4 SPKK217 pKa = 10.31 NIQEE221 pKa = 4.26 FLSSS225 pKa = 3.31
Molecular weight: 25.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.489
IPC2_protein 11.082
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.427
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.135
IPC_peptide 13.364
IPC2_peptide 12.34
IPC2.peptide.svr19 9.152
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
119
0
119
35198
72
1041
295.8
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.248 ± 0.208
1.918 ± 0.134
6.586 ± 0.193
6.424 ± 0.203
5.04 ± 0.165
5.037 ± 0.162
1.827 ± 0.103
5.983 ± 0.17
7.157 ± 0.185
7.989 ± 0.161
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.099
5.483 ± 0.178
3.872 ± 0.126
2.818 ± 0.125
6.12 ± 0.253
6.799 ± 0.229
5.361 ± 0.144
7.992 ± 0.158
0.943 ± 0.056
3.682 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here