Streptococcus satellite phage Javan354
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKW3|A0A4D5ZKW3_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan354 OX=2558655 GN=JavanS354_0001 PE=3 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.13 LWEE5 pKa = 4.2 YY6 pKa = 10.59 VDD8 pKa = 3.58 KK9 pKa = 11.07 NVRR12 pKa = 11.84 LVLSDD17 pKa = 3.11 GTSIVGKK24 pKa = 10.48 VIDD27 pKa = 4.19 WFDD30 pKa = 3.9 GYY32 pKa = 11.41 DD33 pKa = 3.46 LDD35 pKa = 5.95 GYY37 pKa = 10.93 DD38 pKa = 4.68 EE39 pKa = 4.82 IVIDD43 pKa = 4.64 DD44 pKa = 3.86 YY45 pKa = 11.9 SYY47 pKa = 11.48 SEE49 pKa = 4.12 SSIKK53 pKa = 10.33 EE54 pKa = 3.54 IEE56 pKa = 4.22 IVKK59 pKa = 10.56 GEE61 pKa = 3.91 QLNSRR66 pKa = 11.84 KK67 pKa = 9.08 EE68 pKa = 3.89 VV69 pKa = 3.21
Molecular weight: 8.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.318
IPC2_protein 4.202
IPC_protein 4.113
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.948
Grimsley 3.834
Solomon 4.075
Lehninger 4.037
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.228
Patrickios 3.694
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.158
Protein with the highest isoelectric point:
>tr|A0A4D5ZKE6|A0A4D5ZKE6_9VIRU RelE/StbE replicon stabilization toxin OS=Streptococcus satellite phage Javan354 OX=2558655 GN=JavanS354_0013 PE=4 SV=1
MM1 pKa = 7.55 LISVEE6 pKa = 3.8 IAEE9 pKa = 4.43 KK10 pKa = 10.7 VRR12 pKa = 11.84 AKK14 pKa = 10.23 RR15 pKa = 11.84 GKK17 pKa = 10.17 RR18 pKa = 11.84 NLTKK22 pKa = 10.67 SQTALALGIARR33 pKa = 11.84 TTLSKK38 pKa = 11.07 VEE40 pKa = 4.19 SGNYY44 pKa = 8.03 NAPKK48 pKa = 10.1 RR49 pKa = 11.84 IYY51 pKa = 9.06 EE52 pKa = 4.14 AVMNWLVEE60 pKa = 4.22 DD61 pKa = 4.39 LL62 pKa = 4.42
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.099
IPC2_protein 9.355
IPC_protein 9.428
Toseland 10.248
ProMoST 9.809
Dawson 10.379
Bjellqvist 9.984
Wikipedia 10.511
Rodwell 10.906
Grimsley 10.423
Solomon 10.423
Lehninger 10.409
Nozaki 10.204
DTASelect 9.984
Thurlkill 10.248
EMBOSS 10.628
Sillero 10.277
Patrickios 10.716
IPC_peptide 10.423
IPC2_peptide 8.434
IPC2.peptide.svr19 8.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2462
37
482
144.8
16.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.296 ± 0.553
0.406 ± 0.111
5.727 ± 0.919
8.002 ± 0.773
4.427 ± 0.583
5.321 ± 0.294
1.706 ± 0.285
6.824 ± 0.43
8.652 ± 0.653
11.454 ± 1.163
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.234 ± 0.27
5.321 ± 0.488
2.518 ± 0.268
3.452 ± 0.526
5.321 ± 0.565
5.524 ± 0.524
4.996 ± 0.557
5.808 ± 0.345
1.219 ± 0.191
4.793 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here