Escherichia phage vB_EcoP_G7C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus G7C

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0XNR6|G0XNR6_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_G7C OX=1054461 GN=gp17.1 PE=4 SV=1
MM1 pKa = 7.32PTLKK5 pKa = 10.32IAFNKK10 pKa = 5.89TTNVAIVLDD19 pKa = 4.19SDD21 pKa = 4.11GSVPDD26 pKa = 3.94GFAEE30 pKa = 4.08VGTFVHH36 pKa = 7.29PDD38 pKa = 2.76ATYY41 pKa = 10.1PDD43 pKa = 4.13SLVIYY48 pKa = 10.11HH49 pKa = 6.49GVRR52 pKa = 11.84DD53 pKa = 3.89LLYY56 pKa = 10.39KK57 pKa = 10.61RR58 pKa = 11.84SAEE61 pKa = 4.14DD62 pKa = 3.25PAEE65 pKa = 4.12EE66 pKa = 4.56GFWPNSIVDD75 pKa = 3.88MQSITITPQLTIATEE90 pKa = 3.99LLNEE94 pKa = 4.1VSAIEE99 pKa = 4.29GEE101 pKa = 4.32DD102 pKa = 3.71INWHH106 pKa = 6.04IDD108 pKa = 3.44VAGGKK113 pKa = 10.11APFTYY118 pKa = 9.64KK119 pKa = 9.37WQYY122 pKa = 10.44KK123 pKa = 10.37ADD125 pKa = 3.64TDD127 pKa = 4.03DD128 pKa = 5.79AVFTDD133 pKa = 4.39IDD135 pKa = 3.95SDD137 pKa = 4.35DD138 pKa = 4.32NEE140 pKa = 4.52SAATATLTISGVTAASAGTYY160 pKa = 9.89KK161 pKa = 10.98VIVTDD166 pKa = 4.1ANGTTVEE173 pKa = 4.31DD174 pKa = 3.9TSLLTVSYY182 pKa = 10.07PEE184 pKa = 4.31ASSLVATPDD193 pKa = 3.44SLEE196 pKa = 4.44LSVADD201 pKa = 4.26DD202 pKa = 3.71TTDD205 pKa = 3.11GKK207 pKa = 9.16TVTITALPVGASVGTLSIKK226 pKa = 8.55TAPDD230 pKa = 3.17SARR233 pKa = 11.84ATATISGNVLTVKK246 pKa = 10.32PVAEE250 pKa = 4.61GEE252 pKa = 4.22ATSVVVTNGTVDD264 pKa = 3.19VTIAITVAEE273 pKa = 4.21

Molecular weight: 28.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0XNQ1|G0XNQ1_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_G7C OX=1054461 GN=gp2.3 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84VLCNRR7 pKa = 11.84YY8 pKa = 9.51NPAKK12 pKa = 10.32LIDD15 pKa = 3.93FMEE18 pKa = 5.35PIGSRR23 pKa = 11.84MTYY26 pKa = 8.52AARR29 pKa = 11.84WQVSIADD36 pKa = 3.86SRR38 pKa = 11.84EE39 pKa = 3.38RR40 pKa = 11.84TYY42 pKa = 11.3DD43 pKa = 3.24YY44 pKa = 11.19FFVAKK49 pKa = 7.75PTRR52 pKa = 11.84KK53 pKa = 9.58QIRR56 pKa = 11.84KK57 pKa = 7.77LHH59 pKa = 6.06KK60 pKa = 10.06GG61 pKa = 3.23

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

22411

37

3748

283.7

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.679 ± 0.553

0.901 ± 0.162

5.765 ± 0.144

6.269 ± 0.314

3.543 ± 0.154

6.765 ± 0.267

1.771 ± 0.166

5.743 ± 0.234

6.421 ± 0.302

8.139 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.012 ± 0.133

5.814 ± 0.175

4.065 ± 0.148

4.266 ± 0.274

4.453 ± 0.167

6.202 ± 0.207

6.479 ± 0.276

6.765 ± 0.252

1.2 ± 0.12

3.748 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski