Amino acid dipepetide frequency for Escherichia phage vB_EcoP_G7C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.434AlaAla: 8.434 ± 1.174
0.669AlaCys: 0.669 ± 0.204
4.552AlaAsp: 4.552 ± 0.614
5.087AlaGlu: 5.087 ± 0.475
2.454AlaPhe: 2.454 ± 0.324
6.783AlaGly: 6.783 ± 0.616
1.249AlaHis: 1.249 ± 0.228
5.042AlaIle: 5.042 ± 0.486
5.489AlaLys: 5.489 ± 0.61
7.184AlaLeu: 7.184 ± 0.893
3.079AlaMet: 3.079 ± 0.43
5.221AlaAsn: 5.221 ± 0.565
2.454AlaPro: 2.454 ± 0.461
4.462AlaGln: 4.462 ± 0.822
3.57AlaArg: 3.57 ± 0.472
4.552AlaSer: 4.552 ± 0.475
6.247AlaThr: 6.247 ± 0.573
5.622AlaVal: 5.622 ± 0.48
1.205AlaTrp: 1.205 ± 0.238
3.436AlaTyr: 3.436 ± 0.381
0.0AlaXaa: 0.0 ± 0.0
Cys
0.535CysAla: 0.535 ± 0.171
0.045CysCys: 0.045 ± 0.04
0.402CysAsp: 0.402 ± 0.151
0.759CysGlu: 0.759 ± 0.209
0.402CysPhe: 0.402 ± 0.134
0.402CysGly: 0.402 ± 0.153
0.223CysHis: 0.223 ± 0.093
0.848CysIle: 0.848 ± 0.248
0.669CysLys: 0.669 ± 0.196
0.58CysLeu: 0.58 ± 0.191
0.357CysMet: 0.357 ± 0.124
0.669CysAsn: 0.669 ± 0.18
0.357CysPro: 0.357 ± 0.163
0.134CysGln: 0.134 ± 0.097
0.491CysArg: 0.491 ± 0.162
0.58CysSer: 0.58 ± 0.156
0.491CysThr: 0.491 ± 0.186
0.714CysVal: 0.714 ± 0.239
0.134CysTrp: 0.134 ± 0.071
0.223CysTyr: 0.223 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
5.042AspAla: 5.042 ± 0.548
0.937AspCys: 0.937 ± 0.233
3.168AspAsp: 3.168 ± 0.371
3.257AspGlu: 3.257 ± 0.508
2.767AspPhe: 2.767 ± 0.385
3.793AspGly: 3.793 ± 0.358
0.982AspHis: 0.982 ± 0.255
4.418AspIle: 4.418 ± 0.491
3.481AspLys: 3.481 ± 0.44
4.462AspLeu: 4.462 ± 0.552
1.696AspMet: 1.696 ± 0.266
2.499AspAsn: 2.499 ± 0.277
2.41AspPro: 2.41 ± 0.303
2.008AspGln: 2.008 ± 0.344
2.544AspArg: 2.544 ± 0.275
3.927AspSer: 3.927 ± 0.359
3.838AspThr: 3.838 ± 0.406
3.347AspVal: 3.347 ± 0.368
0.759AspTrp: 0.759 ± 0.204
2.32AspTyr: 2.32 ± 0.349
0.0AspXaa: 0.0 ± 0.0
Glu
6.961GluAla: 6.961 ± 0.883
0.312GluCys: 0.312 ± 0.134
3.793GluAsp: 3.793 ± 0.535
5.578GluGlu: 5.578 ± 0.735
2.41GluPhe: 2.41 ± 0.372
3.748GluGly: 3.748 ± 0.446
0.803GluHis: 0.803 ± 0.195
2.9GluIle: 2.9 ± 0.404
3.436GluLys: 3.436 ± 0.43
5.176GluLeu: 5.176 ± 0.471
2.142GluMet: 2.142 ± 0.313
2.9GluAsn: 2.9 ± 0.328
3.257GluPro: 3.257 ± 0.474
2.767GluGln: 2.767 ± 0.448
1.874GluArg: 1.874 ± 0.314
3.257GluSer: 3.257 ± 0.437
2.856GluThr: 2.856 ± 0.323
4.775GluVal: 4.775 ± 0.478
0.759GluTrp: 0.759 ± 0.16
2.99GluTyr: 2.99 ± 0.435
0.0GluXaa: 0.0 ± 0.0
Phe
2.231PheAla: 2.231 ± 0.316
0.491PheCys: 0.491 ± 0.176
2.187PheAsp: 2.187 ± 0.395
1.919PheGlu: 1.919 ± 0.265
1.071PhePhe: 1.071 ± 0.265
2.767PheGly: 2.767 ± 0.334
0.669PheHis: 0.669 ± 0.173
2.365PheIle: 2.365 ± 0.342
1.874PheLys: 1.874 ± 0.321
2.677PheLeu: 2.677 ± 0.322
1.785PheMet: 1.785 ± 0.301
2.231PheAsn: 2.231 ± 0.337
1.16PhePro: 1.16 ± 0.231
1.294PheGln: 1.294 ± 0.272
1.785PheArg: 1.785 ± 0.311
2.454PheSer: 2.454 ± 0.301
3.034PheThr: 3.034 ± 0.336
1.919PheVal: 1.919 ± 0.309
0.268PheTrp: 0.268 ± 0.106
1.249PheTyr: 1.249 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
5.712GlyAla: 5.712 ± 0.611
0.982GlyCys: 0.982 ± 0.237
3.436GlyAsp: 3.436 ± 0.389
3.927GlyGlu: 3.927 ± 0.366
2.499GlyPhe: 2.499 ± 0.434
3.704GlyGly: 3.704 ± 0.562
0.937GlyHis: 0.937 ± 0.228
3.748GlyIle: 3.748 ± 0.412
6.158GlyLys: 6.158 ± 0.598
5.399GlyLeu: 5.399 ± 0.628
2.365GlyMet: 2.365 ± 0.305
4.685GlyAsn: 4.685 ± 0.562
0.759GlyPro: 0.759 ± 0.183
2.499GlyGln: 2.499 ± 0.312
2.454GlyArg: 2.454 ± 0.457
4.864GlySer: 4.864 ± 0.5
4.328GlyThr: 4.328 ± 0.469
4.864GlyVal: 4.864 ± 0.573
0.937GlyTrp: 0.937 ± 0.186
3.391GlyTyr: 3.391 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
1.249HisAla: 1.249 ± 0.254
0.178HisCys: 0.178 ± 0.07
1.026HisAsp: 1.026 ± 0.271
1.026HisGlu: 1.026 ± 0.247
0.312HisPhe: 0.312 ± 0.133
1.026HisGly: 1.026 ± 0.288
0.446HisHis: 0.446 ± 0.147
1.339HisIle: 1.339 ± 0.263
1.294HisLys: 1.294 ± 0.27
2.008HisLeu: 2.008 ± 0.372
0.312HisMet: 0.312 ± 0.112
1.116HisAsn: 1.116 ± 0.218
0.669HisPro: 0.669 ± 0.187
0.491HisGln: 0.491 ± 0.193
0.669HisArg: 0.669 ± 0.185
1.339HisSer: 1.339 ± 0.266
1.026HisThr: 1.026 ± 0.249
0.937HisVal: 0.937 ± 0.225
0.357HisTrp: 0.357 ± 0.154
0.892HisTyr: 0.892 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
4.552IleAla: 4.552 ± 0.408
0.446IleCys: 0.446 ± 0.14
3.882IleAsp: 3.882 ± 0.573
3.57IleGlu: 3.57 ± 0.351
1.606IlePhe: 1.606 ± 0.332
3.079IleGly: 3.079 ± 0.348
1.116IleHis: 1.116 ± 0.268
2.945IleIle: 2.945 ± 0.493
3.971IleLys: 3.971 ± 0.47
3.882IleLeu: 3.882 ± 0.463
0.982IleMet: 0.982 ± 0.234
3.838IleAsn: 3.838 ± 0.544
3.525IlePro: 3.525 ± 0.474
2.231IleGln: 2.231 ± 0.266
3.347IleArg: 3.347 ± 0.325
3.213IleSer: 3.213 ± 0.332
4.507IleThr: 4.507 ± 0.404
3.659IleVal: 3.659 ± 0.382
0.491IleTrp: 0.491 ± 0.168
2.588IleTyr: 2.588 ± 0.35
0.0IleXaa: 0.0 ± 0.0
Lys
6.47LysAla: 6.47 ± 0.552
0.268LysCys: 0.268 ± 0.108
3.168LysAsp: 3.168 ± 0.383
4.552LysGlu: 4.552 ± 0.453
2.454LysPhe: 2.454 ± 0.323
3.793LysGly: 3.793 ± 0.425
1.473LysHis: 1.473 ± 0.33
2.811LysIle: 2.811 ± 0.305
3.436LysLys: 3.436 ± 0.51
6.336LysLeu: 6.336 ± 0.5
1.74LysMet: 1.74 ± 0.379
3.882LysAsn: 3.882 ± 0.377
3.436LysPro: 3.436 ± 0.536
3.124LysGln: 3.124 ± 0.407
2.767LysArg: 2.767 ± 0.36
3.838LysSer: 3.838 ± 0.487
4.284LysThr: 4.284 ± 0.47
3.838LysVal: 3.838 ± 0.412
0.759LysTrp: 0.759 ± 0.234
1.74LysTyr: 1.74 ± 0.339
0.0LysXaa: 0.0 ± 0.0
Leu
7.675LeuAla: 7.675 ± 0.702
0.803LeuCys: 0.803 ± 0.214
4.596LeuAsp: 4.596 ± 0.471
4.328LeuGlu: 4.328 ± 0.407
3.079LeuPhe: 3.079 ± 0.349
6.024LeuGly: 6.024 ± 0.422
1.473LeuHis: 1.473 ± 0.28
4.373LeuIle: 4.373 ± 0.414
4.998LeuLys: 4.998 ± 0.37
5.578LeuLeu: 5.578 ± 0.71
2.722LeuMet: 2.722 ± 0.431
5.31LeuAsn: 5.31 ± 0.528
4.016LeuPro: 4.016 ± 0.441
3.257LeuGln: 3.257 ± 0.402
3.793LeuArg: 3.793 ± 0.509
5.533LeuSer: 5.533 ± 0.515
5.132LeuThr: 5.132 ± 0.411
5.533LeuVal: 5.533 ± 0.596
0.714LeuTrp: 0.714 ± 0.196
2.454LeuTyr: 2.454 ± 0.333
0.0LeuXaa: 0.0 ± 0.0
Met
2.588MetAla: 2.588 ± 0.409
0.178MetCys: 0.178 ± 0.083
1.339MetAsp: 1.339 ± 0.251
2.053MetGlu: 2.053 ± 0.314
0.58MetPhe: 0.58 ± 0.178
2.008MetGly: 2.008 ± 0.271
0.357MetHis: 0.357 ± 0.147
2.008MetIle: 2.008 ± 0.298
2.499MetLys: 2.499 ± 0.367
2.454MetLeu: 2.454 ± 0.316
0.759MetMet: 0.759 ± 0.201
2.231MetAsn: 2.231 ± 0.276
0.937MetPro: 0.937 ± 0.272
1.562MetGln: 1.562 ± 0.306
1.428MetArg: 1.428 ± 0.209
2.365MetSer: 2.365 ± 0.32
2.053MetThr: 2.053 ± 0.333
1.74MetVal: 1.74 ± 0.263
0.223MetTrp: 0.223 ± 0.091
0.759MetTyr: 0.759 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
4.418AsnAla: 4.418 ± 0.547
0.491AsnCys: 0.491 ± 0.191
3.079AsnAsp: 3.079 ± 0.462
3.257AsnGlu: 3.257 ± 0.437
1.874AsnPhe: 1.874 ± 0.25
4.552AsnGly: 4.552 ± 0.616
1.428AsnHis: 1.428 ± 0.262
3.436AsnIle: 3.436 ± 0.418
3.57AsnLys: 3.57 ± 0.334
4.507AsnLeu: 4.507 ± 0.463
1.562AsnMet: 1.562 ± 0.24
2.856AsnAsn: 2.856 ± 0.332
3.391AsnPro: 3.391 ± 0.414
3.436AsnGln: 3.436 ± 0.455
3.213AsnArg: 3.213 ± 0.367
3.168AsnSer: 3.168 ± 0.399
3.57AsnThr: 3.57 ± 0.507
3.347AsnVal: 3.347 ± 0.401
0.803AsnTrp: 0.803 ± 0.216
2.187AsnTyr: 2.187 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
3.704ProAla: 3.704 ± 0.348
0.223ProCys: 0.223 ± 0.109
2.767ProAsp: 2.767 ± 0.395
3.927ProGlu: 3.927 ± 0.431
1.919ProPhe: 1.919 ± 0.339
2.454ProGly: 2.454 ± 0.372
0.312ProHis: 0.312 ± 0.128
2.142ProIle: 2.142 ± 0.327
1.874ProLys: 1.874 ± 0.248
3.168ProLeu: 3.168 ± 0.332
1.116ProMet: 1.116 ± 0.148
1.963ProAsn: 1.963 ± 0.26
0.803ProPro: 0.803 ± 0.299
1.339ProGln: 1.339 ± 0.236
1.16ProArg: 1.16 ± 0.262
2.677ProSer: 2.677 ± 0.329
3.257ProThr: 3.257 ± 0.322
3.971ProVal: 3.971 ± 0.503
0.491ProTrp: 0.491 ± 0.14
1.383ProTyr: 1.383 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
5.087GlnAla: 5.087 ± 0.789
0.223GlnCys: 0.223 ± 0.105
2.053GlnAsp: 2.053 ± 0.365
2.945GlnGlu: 2.945 ± 0.297
1.473GlnPhe: 1.473 ± 0.209
2.811GlnGly: 2.811 ± 0.423
0.357GlnHis: 0.357 ± 0.116
2.231GlnIle: 2.231 ± 0.269
2.856GlnLys: 2.856 ± 0.438
3.481GlnLeu: 3.481 ± 0.412
1.16GlnMet: 1.16 ± 0.196
1.83GlnAsn: 1.83 ± 0.407
1.249GlnPro: 1.249 ± 0.297
1.785GlnGln: 1.785 ± 0.297
1.83GlnArg: 1.83 ± 0.285
3.124GlnSer: 3.124 ± 0.321
2.945GlnThr: 2.945 ± 0.331
2.856GlnVal: 2.856 ± 0.287
0.491GlnTrp: 0.491 ± 0.153
1.874GlnTyr: 1.874 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
3.614ArgAla: 3.614 ± 0.479
0.491ArgCys: 0.491 ± 0.178
2.187ArgAsp: 2.187 ± 0.245
2.99ArgGlu: 2.99 ± 0.407
1.473ArgPhe: 1.473 ± 0.227
2.588ArgGly: 2.588 ± 0.263
0.848ArgHis: 0.848 ± 0.223
2.677ArgIle: 2.677 ± 0.385
3.525ArgLys: 3.525 ± 0.423
4.462ArgLeu: 4.462 ± 0.556
1.606ArgMet: 1.606 ± 0.261
3.079ArgAsn: 3.079 ± 0.385
1.428ArgPro: 1.428 ± 0.224
1.963ArgGln: 1.963 ± 0.326
1.963ArgArg: 1.963 ± 0.339
2.677ArgSer: 2.677 ± 0.349
2.454ArgThr: 2.454 ± 0.316
2.544ArgVal: 2.544 ± 0.333
0.535ArgTrp: 0.535 ± 0.166
1.428ArgTyr: 1.428 ± 0.214
0.0ArgXaa: 0.0 ± 0.0
Ser
4.507SerAla: 4.507 ± 0.468
0.491SerCys: 0.491 ± 0.16
3.927SerAsp: 3.927 ± 0.338
3.57SerGlu: 3.57 ± 0.416
1.963SerPhe: 1.963 ± 0.262
5.132SerGly: 5.132 ± 0.498
1.16SerHis: 1.16 ± 0.2
3.704SerIle: 3.704 ± 0.374
4.195SerLys: 4.195 ± 0.437
6.381SerLeu: 6.381 ± 0.618
1.517SerMet: 1.517 ± 0.223
2.677SerAsn: 2.677 ± 0.376
2.454SerPro: 2.454 ± 0.467
2.454SerGln: 2.454 ± 0.407
2.9SerArg: 2.9 ± 0.373
3.838SerSer: 3.838 ± 0.586
4.061SerThr: 4.061 ± 0.52
4.328SerVal: 4.328 ± 0.412
0.759SerTrp: 0.759 ± 0.192
2.008SerTyr: 2.008 ± 0.325
0.0SerXaa: 0.0 ± 0.0
Thr
4.507ThrAla: 4.507 ± 0.494
0.402ThrCys: 0.402 ± 0.184
4.016ThrAsp: 4.016 ± 0.453
3.614ThrGlu: 3.614 ± 0.402
2.856ThrPhe: 2.856 ± 0.278
4.819ThrGly: 4.819 ± 0.405
1.026ThrHis: 1.026 ± 0.309
4.373ThrIle: 4.373 ± 0.443
3.614ThrLys: 3.614 ± 0.365
5.176ThrLeu: 5.176 ± 0.474
1.517ThrMet: 1.517 ± 0.247
4.284ThrAsn: 4.284 ± 0.592
3.659ThrPro: 3.659 ± 0.417
2.722ThrGln: 2.722 ± 0.299
2.9ThrArg: 2.9 ± 0.344
3.748ThrSer: 3.748 ± 0.428
3.704ThrThr: 3.704 ± 0.635
4.775ThrVal: 4.775 ± 0.546
0.803ThrTrp: 0.803 ± 0.231
2.276ThrTyr: 2.276 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
5.622ValAla: 5.622 ± 0.775
0.669ValCys: 0.669 ± 0.181
4.061ValAsp: 4.061 ± 0.404
3.882ValGlu: 3.882 ± 0.398
2.231ValPhe: 2.231 ± 0.372
4.864ValGly: 4.864 ± 0.687
1.562ValHis: 1.562 ± 0.19
3.213ValIle: 3.213 ± 0.5
3.882ValLys: 3.882 ± 0.323
4.953ValLeu: 4.953 ± 0.449
2.231ValMet: 2.231 ± 0.331
4.016ValAsn: 4.016 ± 0.375
2.811ValPro: 2.811 ± 0.295
3.034ValGln: 3.034 ± 0.359
3.481ValArg: 3.481 ± 0.359
3.971ValSer: 3.971 ± 0.413
4.953ValThr: 4.953 ± 0.583
4.819ValVal: 4.819 ± 0.57
0.848ValTrp: 0.848 ± 0.171
2.544ValTyr: 2.544 ± 0.353
0.0ValXaa: 0.0 ± 0.0
Trp
0.937TrpAla: 0.937 ± 0.227
0.223TrpCys: 0.223 ± 0.09
1.116TrpAsp: 1.116 ± 0.271
0.535TrpGlu: 0.535 ± 0.193
0.357TrpPhe: 0.357 ± 0.126
0.759TrpGly: 0.759 ± 0.151
0.402TrpHis: 0.402 ± 0.145
0.714TrpIle: 0.714 ± 0.199
0.803TrpLys: 0.803 ± 0.177
1.116TrpLeu: 1.116 ± 0.259
0.268TrpMet: 0.268 ± 0.098
0.535TrpAsn: 0.535 ± 0.19
0.357TrpPro: 0.357 ± 0.115
0.535TrpGln: 0.535 ± 0.122
0.491TrpArg: 0.491 ± 0.121
0.759TrpSer: 0.759 ± 0.169
0.357TrpThr: 0.357 ± 0.126
1.294TrpVal: 1.294 ± 0.213
0.045TrpTrp: 0.045 ± 0.047
0.402TrpTyr: 0.402 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.945TyrAla: 2.945 ± 0.325
0.491TyrCys: 0.491 ± 0.159
2.9TyrAsp: 2.9 ± 0.346
1.919TyrGlu: 1.919 ± 0.337
1.651TyrPhe: 1.651 ± 0.317
2.544TyrGly: 2.544 ± 0.332
0.892TyrHis: 0.892 ± 0.233
2.142TyrIle: 2.142 ± 0.313
2.588TyrLys: 2.588 ± 0.38
2.41TyrLeu: 2.41 ± 0.299
1.16TyrMet: 1.16 ± 0.211
2.32TyrAsn: 2.32 ± 0.265
1.517TyrPro: 1.517 ± 0.318
1.562TyrGln: 1.562 ± 0.234
1.874TyrArg: 1.874 ± 0.374
2.142TyrSer: 2.142 ± 0.35
1.696TyrThr: 1.696 ± 0.299
2.767TyrVal: 2.767 ± 0.347
0.625TyrTrp: 0.625 ± 0.205
1.339TyrTyr: 1.339 ± 0.276
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (22411 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski