Bat betaherpesvirus B7D8
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 189 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3VQ95|I3VQ95_9BETA B101.1 OS=Bat betaherpesvirus B7D8 OX=1195167 PE=4 SV=1
MM1 pKa = 7.34 SGKK4 pKa = 10.43 SSVCFASGMEE14 pKa = 4.63 VILDD18 pKa = 4.31 CNDD21 pKa = 3.16 GDD23 pKa = 3.83 QTVFFALTEE32 pKa = 4.41 DD33 pKa = 3.27 SSPRR37 pKa = 11.84 VISRR41 pKa = 11.84 NNLPSNRR48 pKa = 11.84 RR49 pKa = 11.84 FIVLAEE55 pKa = 4.28 EE56 pKa = 4.75 DD57 pKa = 3.63 PCGNDD62 pKa = 3.14 MSQGEE67 pKa = 4.27 PNEE70 pKa = 4.14 PFVEE74 pKa = 4.41 DD75 pKa = 3.06 VDD77 pKa = 5.12 GEE79 pKa = 4.72 SEE81 pKa = 4.55 CFNALDD87 pKa = 4.18 SYY89 pKa = 11.72 NALFDD94 pKa = 3.83 GQIEE98 pKa = 4.77 GFFDD102 pKa = 5.23 DD103 pKa = 4.71 YY104 pKa = 11.97 LNLEE108 pKa = 4.28 PDD110 pKa = 3.29 ACVTRR115 pKa = 11.84 LDD117 pKa = 3.57 VEE119 pKa = 4.5 MYY121 pKa = 10.91 DD122 pKa = 4.85 LLTEE126 pKa = 4.45 CGEE129 pKa = 4.27 DD130 pKa = 3.41 ACVSACDD137 pKa = 3.12 RR138 pKa = 11.84 WADD141 pKa = 4.63 DD142 pKa = 4.2 RR143 pKa = 11.84 ADD145 pKa = 3.42 SCGFQLL151 pKa = 5.3
Molecular weight: 16.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.554
IPC_protein 3.554
Toseland 3.338
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.49
Rodwell 3.376
Grimsley 3.249
Solomon 3.528
Lehninger 3.49
Nozaki 3.668
DTASelect 3.897
Thurlkill 3.401
EMBOSS 3.503
Sillero 3.681
Patrickios 0.477
IPC_peptide 3.528
IPC2_peptide 3.643
IPC2.peptide.svr19 3.63
Protein with the highest isoelectric point:
>tr|I3VQ62|I3VQ62_9BETA B69.2 OS=Bat betaherpesvirus B7D8 OX=1195167 PE=4 SV=1
MM1 pKa = 7.84 RR2 pKa = 11.84 ATSRR6 pKa = 11.84 RR7 pKa = 11.84 SKK9 pKa = 10.43 PYY11 pKa = 10.93 NKK13 pKa = 9.95 YY14 pKa = 8.71 CTPAPLNKK22 pKa = 10.01 SCFHH26 pKa = 6.24 RR27 pKa = 11.84 WLYY30 pKa = 11.17 LFAFFFFGDD39 pKa = 4.14 LEE41 pKa = 4.42 RR42 pKa = 11.84 MRR44 pKa = 11.84 RR45 pKa = 11.84 TRR47 pKa = 11.84 AVCADD52 pKa = 3.74 RR53 pKa = 11.84 DD54 pKa = 4.33 FISFFF59 pKa = 4.04
Molecular weight: 7.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.648
IPC_protein 10.438
Toseland 10.335
ProMoST 10.116
Dawson 10.526
Bjellqvist 10.292
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.599
Solomon 10.613
Lehninger 10.57
Nozaki 10.409
DTASelect 10.262
Thurlkill 10.394
EMBOSS 10.745
Sillero 10.467
Patrickios 10.452
IPC_peptide 10.613
IPC2_peptide 9.619
IPC2.peptide.svr19 8.351
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
189
0
189
63743
50
2171
337.3
37.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.182 ± 0.146
2.832 ± 0.122
6.143 ± 0.127
5.196 ± 0.128
4.264 ± 0.1
6.266 ± 0.232
2.279 ± 0.074
5.072 ± 0.144
3.922 ± 0.122
8.556 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.076
4.048 ± 0.126
4.681 ± 0.16
2.505 ± 0.1
7.883 ± 0.185
7.8 ± 0.145
6.349 ± 0.22
7.717 ± 0.191
1.162 ± 0.059
3.674 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here