Garlic latent virus
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8QXY6|Q8QXY6_9VIRU Movement protein TGB2 OS=Garlic latent virus OX=12458 PE=3 SV=1
MM1 pKa = 7.24 QLQPLIALLIGLSVTLIICLTFDD24 pKa = 3.88 SIRR27 pKa = 11.84 TEE29 pKa = 3.74 RR30 pKa = 11.84 CTVIITGEE38 pKa = 4.16 SVKK41 pKa = 10.65 FLGCEE46 pKa = 3.91 FTRR49 pKa = 11.84 DD50 pKa = 4.0 FIDD53 pKa = 4.11 FAIQAKK59 pKa = 9.97 PFGSLL64 pKa = 3.12
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.042
IPC2_protein 5.347
IPC_protein 4.902
Toseland 4.736
ProMoST 5.003
Dawson 4.838
Bjellqvist 4.991
Wikipedia 4.736
Rodwell 4.736
Grimsley 4.647
Solomon 4.825
Lehninger 4.787
Nozaki 4.965
DTASelect 5.105
Thurlkill 4.762
EMBOSS 4.736
Sillero 5.003
Patrickios 4.075
IPC_peptide 4.838
IPC2_peptide 4.991
IPC2.peptide.svr19 5.154
Protein with the highest isoelectric point:
>tr|Q8QXY4|Q8QXY4_9VIRU Capsid protein OS=Garlic latent virus OX=12458 PE=3 SV=1
MM1 pKa = 7.75 LIKK4 pKa = 10.38 QRR6 pKa = 11.84 TYY8 pKa = 10.94 RR9 pKa = 11.84 RR10 pKa = 11.84 LLRR13 pKa = 11.84 AIFKK17 pKa = 10.5 LHH19 pKa = 5.46 TNKK22 pKa = 10.08 NCVDD26 pKa = 5.24 LIDD29 pKa = 5.06 IIVSKK34 pKa = 10.31 IVCDD38 pKa = 3.38 NAGASRR44 pKa = 11.84 YY45 pKa = 9.69 ARR47 pKa = 11.84 ARR49 pKa = 11.84 RR50 pKa = 11.84 AKK52 pKa = 10.6 SIGRR56 pKa = 11.84 CPRR59 pKa = 11.84 CFRR62 pKa = 11.84 CSPGFSFTKK71 pKa = 10.42 NCDD74 pKa = 3.02 SKK76 pKa = 10.0 TCVPGISYY84 pKa = 10.02 NVKK87 pKa = 9.92 VKK89 pKa = 10.86 DD90 pKa = 4.37 FIVNGVTMM98 pKa = 5.03
Molecular weight: 11.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.458
IPC_protein 9.619
Toseland 10.394
ProMoST 9.97
Dawson 10.511
Bjellqvist 10.189
Wikipedia 10.643
Rodwell 10.95
Grimsley 10.54
Solomon 10.555
Lehninger 10.54
Nozaki 10.452
DTASelect 10.145
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.452
Patrickios 10.701
IPC_peptide 10.57
IPC2_peptide 9.428
IPC2.peptide.svr19 8.325
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2729
64
1924
454.8
51.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.449 ± 1.042
3.554 ± 0.674
4.874 ± 0.558
6.669 ± 1.242
6.449 ± 0.671
6.046 ± 0.49
2.492 ± 0.767
4.984 ± 0.887
6.303 ± 0.699
10.553 ± 0.996
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.869 ± 0.331
5.02 ± 0.944
3.884 ± 0.509
2.455 ± 0.269
5.387 ± 0.823
7.292 ± 0.553
5.716 ± 0.786
6.303 ± 0.693
0.696 ± 0.193
3.005 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here