Thalassotalea euphylliae
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E0U2V0|A0A3E0U2V0_9GAMM Antitoxin OS=Thalassotalea euphylliae OX=1655234 GN=DXX94_11645 PE=3 SV=1
MM1 pKa = 7.3 KK2 pKa = 10.65 NKK4 pKa = 8.42 VTFPLNCIALILASQLTGCGSDD26 pKa = 4.14 GDD28 pKa = 4.12 NTPQIDD34 pKa = 5.33 DD35 pKa = 4.0 SATDD39 pKa = 3.44 NTPPVITLIGNEE51 pKa = 4.45 SIDD54 pKa = 3.59 VVYY57 pKa = 10.98 NAVFGDD63 pKa = 4.4 LGAEE67 pKa = 4.03 AHH69 pKa = 7.12 DD70 pKa = 4.2 EE71 pKa = 4.11 TDD73 pKa = 3.12 GAITVTSQGTIDD85 pKa = 3.25 TAMPGTYY92 pKa = 9.89 EE93 pKa = 3.92 LTYY96 pKa = 9.98 TATDD100 pKa = 3.24 ASGNSSQATRR110 pKa = 11.84 TVVVLEE116 pKa = 4.63 DD117 pKa = 3.71 TSQSATITTPQAPFSLFYY135 pKa = 11.27 DD136 pKa = 4.54 GVLKK140 pKa = 10.85 GADD143 pKa = 3.48 YY144 pKa = 10.12 WSEE147 pKa = 3.93 EE148 pKa = 3.95 PQILSAGMGFDD159 pKa = 4.43 GIIGIDD165 pKa = 3.61 APEE168 pKa = 3.98 LTFDD172 pKa = 3.96 SVLEE176 pKa = 4.26 AGGAWSSDD184 pKa = 3.38 INCGTGAQNYY194 pKa = 7.16 TSTSTLDD201 pKa = 3.58 QIAGAYY207 pKa = 9.17 INQTPYY213 pKa = 11.62 GEE215 pKa = 4.76 LKK217 pKa = 10.83 DD218 pKa = 3.91 GAIGLDD224 pKa = 3.42 GLPIVLSWPIDD235 pKa = 3.52 TSTLSLTDD243 pKa = 3.67 FQFTLNTGDD252 pKa = 3.13 IVRR255 pKa = 11.84 PLAIGPLPNFEE266 pKa = 4.76 DD267 pKa = 3.88 NEE269 pKa = 4.51 RR270 pKa = 11.84 NTPVAFGEE278 pKa = 4.24 FGNRR282 pKa = 11.84 LPSDD286 pKa = 3.65 HH287 pKa = 7.63 PDD289 pKa = 2.61 ARR291 pKa = 11.84 FPIKK295 pKa = 10.78 LEE297 pKa = 3.79 IVEE300 pKa = 5.24 DD301 pKa = 4.07 DD302 pKa = 4.1 TPLMMVGPGGQVVSAVGLTWEE323 pKa = 4.79 TDD325 pKa = 3.06 SSPYY329 pKa = 10.52 DD330 pKa = 3.71 EE331 pKa = 5.54 NNGPRR336 pKa = 11.84 LVGAKK341 pKa = 9.94 LNRR344 pKa = 11.84 IEE346 pKa = 4.09 GQMDD350 pKa = 3.9 GEE352 pKa = 5.08 GISTPIPLIPANDD365 pKa = 3.37 ATVLYY370 pKa = 10.79 DD371 pKa = 3.94 EE372 pKa = 5.2 GDD374 pKa = 3.17 FMLRR378 pKa = 11.84 MLTTGGFSPDD388 pKa = 3.4 GVSGLTPNDD397 pKa = 3.17 FEE399 pKa = 5.16 RR400 pKa = 11.84 FFRR403 pKa = 11.84 IHH405 pKa = 6.9 ANGVDD410 pKa = 3.69 GSTVIIDD417 pKa = 3.81 KK418 pKa = 10.97 VGEE421 pKa = 4.07 EE422 pKa = 4.44 YY423 pKa = 10.77 AVQGGTLRR431 pKa = 11.84 VVGMSDD437 pKa = 3.35 LGQPEE442 pKa = 4.15 GGEE445 pKa = 4.42 VVFDD449 pKa = 3.64 ACYY452 pKa = 10.83 SEE454 pKa = 6.09 DD455 pKa = 3.62 RR456 pKa = 11.84 DD457 pKa = 3.83 NYY459 pKa = 10.24 IDD461 pKa = 4.11 IIMVGDD467 pKa = 3.75 EE468 pKa = 3.66 EE469 pKa = 4.19 AARR472 pKa = 11.84 NITILEE478 pKa = 4.16 IPSLEE483 pKa = 4.03 EE484 pKa = 4.56 GYY486 pKa = 10.55 SAFYY490 pKa = 10.93 NPGGPGSTPFEE501 pKa = 4.29 GVSYY505 pKa = 7.74 TAPGPRR511 pKa = 11.84 DD512 pKa = 3.86 LEE514 pKa = 4.16 PVIIALDD521 pKa = 3.5 DD522 pKa = 3.99 PMRR525 pKa = 11.84 VTYY528 pKa = 10.7 VPGSEE533 pKa = 4.37 NVEE536 pKa = 4.26 TPDD539 pKa = 3.9 NLMTFLFEE547 pKa = 4.75 GIEE550 pKa = 4.12 RR551 pKa = 11.84 EE552 pKa = 4.1 YY553 pKa = 10.75 LLNMSSNYY561 pKa = 9.98 TGDD564 pKa = 3.53 TPVPLLFDD572 pKa = 3.37 FHH574 pKa = 7.51 GLNGTAEE581 pKa = 4.11 QQNTDD586 pKa = 3.08 SQFNQLAEE594 pKa = 4.24 TEE596 pKa = 4.16 NFILVTPKK604 pKa = 10.76 AIGGWNVTGFPLGGDD619 pKa = 3.52 ANDD622 pKa = 4.62 LGFIDD627 pKa = 5.05 ALINEE632 pKa = 5.25 LSTTYY637 pKa = 10.84 NIDD640 pKa = 3.22 TNRR643 pKa = 11.84 IYY645 pKa = 10.99 AAGFSLGGFFSFEE658 pKa = 4.08 LACQYY663 pKa = 11.38 SDD665 pKa = 3.16 TFAAIAPVSGVMTPNMANDD684 pKa = 4.38 CLPEE688 pKa = 4.68 RR689 pKa = 11.84 PIPILQTHH697 pKa = 5.99 GTADD701 pKa = 3.56 DD702 pKa = 3.78 QLPYY706 pKa = 10.92 AQAQTVLQWWIDD718 pKa = 3.57 FNQTDD723 pKa = 4.37 VEE725 pKa = 4.13 PAITDD730 pKa = 3.97 LEE732 pKa = 4.45 DD733 pKa = 3.38 TFPDD737 pKa = 3.15 NGTTVQRR744 pKa = 11.84 YY745 pKa = 8.35 VYY747 pKa = 10.86 GNGTNGVSVEE757 pKa = 3.91 HH758 pKa = 6.3 LRR760 pKa = 11.84 IEE762 pKa = 4.74 GGQHH766 pKa = 5.6 IWPGSAGDD774 pKa = 3.78 SDD776 pKa = 4.21 INMAEE781 pKa = 4.41 EE782 pKa = 3.58 IWSFFSNYY790 pKa = 10.0 DD791 pKa = 3.75 LNGKK795 pKa = 9.15 IPDD798 pKa = 3.48
Molecular weight: 85.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.478
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.821
Patrickios 1.24
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A3E0TII7|A0A3E0TII7_9GAMM Carboxy-S-adenosyl-L-methionine synthase OS=Thalassotalea euphylliae OX=1655234 GN=cmoA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.49 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3642
0
3642
1233835
38
8735
338.8
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.219 ± 0.039
0.927 ± 0.014
5.566 ± 0.042
6.145 ± 0.039
4.199 ± 0.027
6.506 ± 0.052
2.211 ± 0.021
6.364 ± 0.029
5.347 ± 0.053
10.234 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.332 ± 0.024
4.636 ± 0.038
3.719 ± 0.025
5.166 ± 0.04
4.143 ± 0.032
6.727 ± 0.04
5.464 ± 0.04
6.9 ± 0.031
1.142 ± 0.014
3.056 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here