Apis mellifera associated microvirus 53

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U641|A0A3Q8U641_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 53 OX=2494784 PE=4 SV=1
MM1 pKa = 6.99KK2 pKa = 9.59THH4 pKa = 7.59FIPDD8 pKa = 3.95LDD10 pKa = 4.02TGEE13 pKa = 5.23LLPKK17 pKa = 10.34HH18 pKa = 6.5VDD20 pKa = 3.09GLEE23 pKa = 3.98QMDD26 pKa = 4.99PNPAAPNLRR35 pKa = 11.84FKK37 pKa = 10.69RR38 pKa = 11.84QPSYY42 pKa = 11.1FEE44 pKa = 3.96QIRR47 pKa = 11.84NQVKK51 pKa = 10.02FEE53 pKa = 4.36LSQAAQDD60 pKa = 3.77KK61 pKa = 10.78GFEE64 pKa = 4.37TFDD67 pKa = 3.41EE68 pKa = 4.84ANDD71 pKa = 3.62FNIGDD76 pKa = 3.88DD77 pKa = 3.9FEE79 pKa = 6.15PYY81 pKa = 9.63SQHH84 pKa = 6.64EE85 pKa = 4.53EE86 pKa = 4.17TFGPDD91 pKa = 3.0GLSDD95 pKa = 3.66FEE97 pKa = 4.22RR98 pKa = 11.84GRR100 pKa = 11.84FVEE103 pKa = 4.46PTQGGDD109 pKa = 3.39PQDD112 pKa = 3.74QEE114 pKa = 5.69GEE116 pKa = 4.35VPPAKK121 pKa = 9.69PSKK124 pKa = 10.87NKK126 pKa = 9.64TPDD129 pKa = 3.63PGQDD133 pKa = 3.34PADD136 pKa = 3.61TT137 pKa = 4.21

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UUB8|A0A3S8UUB8_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 53 OX=2494784 PE=4 SV=1
MM1 pKa = 7.29ATRR4 pKa = 11.84SRR6 pKa = 11.84HH7 pKa = 4.34ARR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 4.21SRR13 pKa = 11.84ARR15 pKa = 11.84KK16 pKa = 9.42QEE18 pKa = 3.83SQTYY22 pKa = 8.81NRR24 pKa = 11.84NEE26 pKa = 3.73RR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.31FATIARR35 pKa = 11.84QSLPNVSIYY44 pKa = 10.81SPSSNYY50 pKa = 9.55IPPRR54 pKa = 11.84YY55 pKa = 9.28YY56 pKa = 10.22NARR59 pKa = 11.84FRR61 pKa = 11.84DD62 pKa = 3.38LSYY65 pKa = 10.7IQDD68 pKa = 3.08RR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.77FNFGEE76 pKa = 4.53RR77 pKa = 11.84YY78 pKa = 8.64PVRR81 pKa = 11.84DD82 pKa = 3.63VYY84 pKa = 11.5GSFPSYY90 pKa = 10.28HH91 pKa = 6.23VPTYY95 pKa = 9.12TPRR98 pKa = 11.84KK99 pKa = 8.65RR100 pKa = 11.84ISRR103 pKa = 11.84TSGGLSPLIRR113 pKa = 11.84YY114 pKa = 8.02DD115 pKa = 3.45DD116 pKa = 4.23PNRR119 pKa = 11.84VLTCLRR125 pKa = 11.84RR126 pKa = 11.84KK127 pKa = 9.39RR128 pKa = 11.84RR129 pKa = 11.84KK130 pKa = 7.84EE131 pKa = 3.67TLFALGVGGSRR142 pKa = 11.84TRR144 pKa = 11.84KK145 pKa = 8.68PKK147 pKa = 10.59NFSKK151 pKa = 11.02QNVRR155 pKa = 11.84CC156 pKa = 3.82

Molecular weight: 18.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1381

137

489

276.2

30.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.386 ± 0.963

0.724 ± 0.375

6.227 ± 0.946

4.852 ± 1.008

4.634 ± 1.175

8.834 ± 0.938

2.534 ± 0.177

4.852 ± 0.577

4.852 ± 0.909

7.096 ± 0.741

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.621

4.055 ± 0.661

6.589 ± 0.915

4.562 ± 0.831

8.182 ± 1.79

7.314 ± 0.781

5.431 ± 0.107

4.634 ± 0.645

0.869 ± 0.276

4.055 ± 0.655
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski