Gordonia phage CherryonLim

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8D9Y7|A0A5P8D9Y7_9CAUD Uncharacterized protein OS=Gordonia phage CherryonLim OX=2652411 GN=51 PE=4 SV=1
MM1 pKa = 7.3NKK3 pKa = 10.2KK4 pKa = 10.46LITILAGVVAACGMTAAPATAMISLDD30 pKa = 3.09AKK32 pKa = 10.61SNVRR36 pKa = 11.84YY37 pKa = 7.78MVCGDD42 pKa = 3.57GVAEE46 pKa = 3.89IEE48 pKa = 4.38YY49 pKa = 10.67NNAYY53 pKa = 10.25GNYY56 pKa = 9.38DD57 pKa = 3.24WADD60 pKa = 2.98VDD62 pKa = 5.38LSQGCWFYY70 pKa = 11.74DD71 pKa = 3.89LYY73 pKa = 11.42NGSDD77 pKa = 3.27EE78 pKa = 4.88FGYY81 pKa = 10.15PDD83 pKa = 3.64GAAWADD89 pKa = 3.64VNVTDD94 pKa = 4.08TNGGYY99 pKa = 9.57VSCVVWVNGVIEE111 pKa = 4.83GRR113 pKa = 11.84ASDD116 pKa = 3.39NSEE119 pKa = 4.01YY120 pKa = 10.91YY121 pKa = 10.62SYY123 pKa = 11.49ASCYY127 pKa = 9.91

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DBL5|A0A5P8DBL5_9CAUD Tail assembly chaperone OS=Gordonia phage CherryonLim OX=2652411 GN=16 PE=4 SV=1
MM1 pKa = 7.31SRR3 pKa = 11.84KK4 pKa = 7.96NQRR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84TDD13 pKa = 2.96PVDD16 pKa = 3.61RR17 pKa = 11.84YY18 pKa = 10.88NNNLIAQVRR27 pKa = 11.84DD28 pKa = 3.8LPAMNMPTNPAIARR42 pKa = 11.84QYY44 pKa = 9.81EE45 pKa = 4.08QLKK48 pKa = 9.79KK49 pKa = 10.44ADD51 pKa = 3.4YY52 pKa = 9.97VLRR55 pKa = 11.84LKK57 pKa = 10.69RR58 pKa = 11.84YY59 pKa = 7.34GFKK62 pKa = 10.5VGKK65 pKa = 7.26EE66 pKa = 4.22TPP68 pKa = 3.33

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

15172

34

1695

210.7

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.656 ± 0.691

1.028 ± 0.145

6.842 ± 0.321

5.998 ± 0.387

3.203 ± 0.201

7.857 ± 0.618

1.991 ± 0.162

4.97 ± 0.139

4.772 ± 0.236

7.903 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.129

3.441 ± 0.209

5.444 ± 0.278

3.816 ± 0.264

6.802 ± 0.289

5.451 ± 0.25

6.097 ± 0.334

7.046 ± 0.213

2.195 ± 0.148

2.795 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski