Amino acid dipepetide frequency for Gordonia phage CherryonLim

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.546AlaAla: 10.546 ± 1.561
0.989AlaCys: 0.989 ± 0.258
6.723AlaAsp: 6.723 ± 0.749
5.471AlaGlu: 5.471 ± 0.577
4.35AlaPhe: 4.35 ± 0.721
7.251AlaGly: 7.251 ± 0.835
1.648AlaHis: 1.648 ± 0.337
4.746AlaIle: 4.746 ± 0.468
3.757AlaLys: 3.757 ± 0.484
7.646AlaLeu: 7.646 ± 0.822
3.955AlaMet: 3.955 ± 0.633
3.098AlaAsn: 3.098 ± 0.501
4.68AlaPro: 4.68 ± 0.607
3.625AlaGln: 3.625 ± 0.769
5.998AlaArg: 5.998 ± 0.783
5.537AlaSer: 5.537 ± 0.662
5.801AlaThr: 5.801 ± 0.571
6.328AlaVal: 6.328 ± 0.803
2.043AlaTrp: 2.043 ± 0.364
2.307AlaTyr: 2.307 ± 0.416
0.0AlaXaa: 0.0 ± 0.0
Cys
0.791CysAla: 0.791 ± 0.273
0.0CysCys: 0.0 ± 0.0
1.186CysAsp: 1.186 ± 0.366
0.659CysGlu: 0.659 ± 0.241
0.395CysPhe: 0.395 ± 0.187
1.055CysGly: 1.055 ± 0.33
0.198CysHis: 0.198 ± 0.111
0.198CysIle: 0.198 ± 0.105
0.527CysLys: 0.527 ± 0.182
0.461CysLeu: 0.461 ± 0.168
0.066CysMet: 0.066 ± 0.062
0.527CysAsn: 0.527 ± 0.197
0.264CysPro: 0.264 ± 0.123
0.33CysGln: 0.33 ± 0.143
0.725CysArg: 0.725 ± 0.197
0.659CysSer: 0.659 ± 0.22
0.791CysThr: 0.791 ± 0.295
0.725CysVal: 0.725 ± 0.259
0.198CysTrp: 0.198 ± 0.094
0.527CysTyr: 0.527 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
5.075AspAla: 5.075 ± 0.426
0.527AspCys: 0.527 ± 0.214
5.273AspAsp: 5.273 ± 0.766
6.46AspGlu: 6.46 ± 0.844
2.109AspPhe: 2.109 ± 0.39
4.614AspGly: 4.614 ± 0.648
1.384AspHis: 1.384 ± 0.303
3.823AspIle: 3.823 ± 0.422
2.505AspLys: 2.505 ± 0.483
5.866AspLeu: 5.866 ± 0.62
1.78AspMet: 1.78 ± 0.322
2.373AspAsn: 2.373 ± 0.358
4.482AspPro: 4.482 ± 0.548
2.307AspGln: 2.307 ± 0.412
4.284AspArg: 4.284 ± 0.479
2.768AspSer: 2.768 ± 0.43
3.889AspThr: 3.889 ± 0.654
4.878AspVal: 4.878 ± 0.501
1.516AspTrp: 1.516 ± 0.247
2.505AspTyr: 2.505 ± 0.412
0.0AspXaa: 0.0 ± 0.0
Glu
5.669GluAla: 5.669 ± 0.475
0.857GluCys: 0.857 ± 0.257
5.01GluAsp: 5.01 ± 0.691
4.35GluGlu: 4.35 ± 0.572
1.186GluPhe: 1.186 ± 0.289
4.614GluGly: 4.614 ± 0.754
1.252GluHis: 1.252 ± 0.291
2.966GluIle: 2.966 ± 0.447
2.505GluLys: 2.505 ± 0.459
5.273GluLeu: 5.273 ± 0.467
1.912GluMet: 1.912 ± 0.425
1.912GluAsn: 1.912 ± 0.353
2.637GluPro: 2.637 ± 0.426
3.032GluGln: 3.032 ± 0.375
4.153GluArg: 4.153 ± 0.585
2.637GluSer: 2.637 ± 0.44
3.164GluThr: 3.164 ± 0.347
3.428GluVal: 3.428 ± 0.589
1.186GluTrp: 1.186 ± 0.266
2.241GluTyr: 2.241 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.637PheAla: 2.637 ± 0.435
0.659PheCys: 0.659 ± 0.244
3.559PheAsp: 3.559 ± 0.533
1.977PheGlu: 1.977 ± 0.322
1.186PhePhe: 1.186 ± 0.271
2.505PheGly: 2.505 ± 0.393
0.791PheHis: 0.791 ± 0.209
1.648PheIle: 1.648 ± 0.361
1.186PheLys: 1.186 ± 0.323
2.043PheLeu: 2.043 ± 0.393
0.791PheMet: 0.791 ± 0.216
1.121PheAsn: 1.121 ± 0.242
1.384PhePro: 1.384 ± 0.268
1.186PheGln: 1.186 ± 0.313
2.373PheArg: 2.373 ± 0.352
1.714PheSer: 1.714 ± 0.389
2.175PheThr: 2.175 ± 0.317
2.373PheVal: 2.373 ± 0.44
0.33PheTrp: 0.33 ± 0.158
0.395PheTyr: 0.395 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
7.317GlyAla: 7.317 ± 1.0
0.395GlyCys: 0.395 ± 0.172
5.735GlyAsp: 5.735 ± 1.244
3.691GlyGlu: 3.691 ± 0.499
2.571GlyPhe: 2.571 ± 0.374
6.064GlyGly: 6.064 ± 0.84
1.384GlyHis: 1.384 ± 0.315
5.075GlyIle: 5.075 ± 0.894
3.625GlyLys: 3.625 ± 0.432
5.603GlyLeu: 5.603 ± 0.495
1.45GlyMet: 1.45 ± 0.333
1.846GlyAsn: 1.846 ± 0.319
4.614GlyPro: 4.614 ± 1.956
2.9GlyGln: 2.9 ± 0.627
5.273GlyArg: 5.273 ± 0.555
4.812GlySer: 4.812 ± 0.675
5.603GlyThr: 5.603 ± 0.673
6.328GlyVal: 6.328 ± 0.711
1.912GlyTrp: 1.912 ± 0.369
2.373GlyTyr: 2.373 ± 0.382
0.0GlyXaa: 0.0 ± 0.0
His
1.186HisAla: 1.186 ± 0.286
0.264HisCys: 0.264 ± 0.128
1.714HisAsp: 1.714 ± 0.324
1.121HisGlu: 1.121 ± 0.317
0.725HisPhe: 0.725 ± 0.268
1.516HisGly: 1.516 ± 0.381
0.527HisHis: 0.527 ± 0.193
0.857HisIle: 0.857 ± 0.232
1.186HisLys: 1.186 ± 0.261
1.055HisLeu: 1.055 ± 0.314
0.593HisMet: 0.593 ± 0.182
0.659HisAsn: 0.659 ± 0.221
1.055HisPro: 1.055 ± 0.282
0.857HisGln: 0.857 ± 0.261
1.45HisArg: 1.45 ± 0.348
1.252HisSer: 1.252 ± 0.26
1.45HisThr: 1.45 ± 0.311
1.582HisVal: 1.582 ± 0.384
0.461HisTrp: 0.461 ± 0.188
0.395HisTyr: 0.395 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
5.801IleAla: 5.801 ± 0.593
0.264IleCys: 0.264 ± 0.131
3.889IleAsp: 3.889 ± 0.521
3.889IleGlu: 3.889 ± 0.482
1.318IlePhe: 1.318 ± 0.327
3.494IleGly: 3.494 ± 0.585
1.121IleHis: 1.121 ± 0.297
2.637IleIle: 2.637 ± 0.421
2.439IleLys: 2.439 ± 0.569
3.098IleLeu: 3.098 ± 0.422
1.318IleMet: 1.318 ± 0.246
1.78IleAsn: 1.78 ± 0.373
2.439IlePro: 2.439 ± 0.339
1.516IleGln: 1.516 ± 0.607
3.164IleArg: 3.164 ± 0.46
2.637IleSer: 2.637 ± 0.382
3.098IleThr: 3.098 ± 0.389
3.757IleVal: 3.757 ± 0.383
0.989IleTrp: 0.989 ± 0.232
1.055IleTyr: 1.055 ± 0.366
0.0IleXaa: 0.0 ± 0.0
Lys
5.339LysAla: 5.339 ± 0.678
0.395LysCys: 0.395 ± 0.158
2.505LysAsp: 2.505 ± 0.334
2.571LysGlu: 2.571 ± 0.412
1.78LysPhe: 1.78 ± 0.425
4.153LysGly: 4.153 ± 0.76
0.791LysHis: 0.791 ± 0.276
2.307LysIle: 2.307 ± 0.364
2.9LysLys: 2.9 ± 0.556
4.087LysLeu: 4.087 ± 0.663
1.252LysMet: 1.252 ± 0.282
1.582LysAsn: 1.582 ± 0.274
2.439LysPro: 2.439 ± 0.412
2.439LysGln: 2.439 ± 0.452
3.164LysArg: 3.164 ± 0.419
2.571LysSer: 2.571 ± 0.534
2.505LysThr: 2.505 ± 0.505
2.505LysVal: 2.505 ± 0.451
0.791LysTrp: 0.791 ± 0.245
1.648LysTyr: 1.648 ± 0.328
0.0LysXaa: 0.0 ± 0.0
Leu
8.701LeuAla: 8.701 ± 1.033
0.791LeuCys: 0.791 ± 0.223
4.614LeuAsp: 4.614 ± 0.533
5.339LeuGlu: 5.339 ± 0.648
1.977LeuPhe: 1.977 ± 0.378
5.866LeuGly: 5.866 ± 0.713
1.78LeuHis: 1.78 ± 0.39
3.494LeuIle: 3.494 ± 0.363
4.219LeuLys: 4.219 ± 0.584
6.592LeuLeu: 6.592 ± 0.621
1.318LeuMet: 1.318 ± 0.278
2.175LeuAsn: 2.175 ± 0.365
5.141LeuPro: 5.141 ± 0.547
2.439LeuGln: 2.439 ± 0.422
6.196LeuArg: 6.196 ± 0.847
4.219LeuSer: 4.219 ± 0.52
4.812LeuThr: 4.812 ± 0.556
4.878LeuVal: 4.878 ± 0.61
1.977LeuTrp: 1.977 ± 0.335
2.505LeuTyr: 2.505 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.703MetAla: 2.703 ± 0.426
0.066MetCys: 0.066 ± 0.067
1.384MetAsp: 1.384 ± 0.258
0.989MetGlu: 0.989 ± 0.221
0.923MetPhe: 0.923 ± 0.223
2.109MetGly: 2.109 ± 0.433
0.593MetHis: 0.593 ± 0.182
1.648MetIle: 1.648 ± 0.424
1.648MetLys: 1.648 ± 0.271
1.78MetLeu: 1.78 ± 0.442
0.659MetMet: 0.659 ± 0.209
0.857MetAsn: 0.857 ± 0.211
1.714MetPro: 1.714 ± 0.299
0.857MetGln: 0.857 ± 0.256
1.582MetArg: 1.582 ± 0.315
2.109MetSer: 2.109 ± 0.42
2.373MetThr: 2.373 ± 0.396
1.714MetVal: 1.714 ± 0.392
0.593MetTrp: 0.593 ± 0.211
0.659MetTyr: 0.659 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
3.625AsnAla: 3.625 ± 0.681
0.527AsnCys: 0.527 ± 0.205
1.648AsnAsp: 1.648 ± 0.318
1.912AsnGlu: 1.912 ± 0.345
1.186AsnPhe: 1.186 ± 0.369
2.834AsnGly: 2.834 ± 0.512
0.659AsnHis: 0.659 ± 0.26
1.45AsnIle: 1.45 ± 0.329
1.516AsnLys: 1.516 ± 0.3
2.637AsnLeu: 2.637 ± 0.444
0.989AsnMet: 0.989 ± 0.308
1.186AsnAsn: 1.186 ± 0.289
2.9AsnPro: 2.9 ± 0.394
0.989AsnGln: 0.989 ± 0.29
2.637AsnArg: 2.637 ± 0.413
2.109AsnSer: 2.109 ± 0.451
1.318AsnThr: 1.318 ± 0.266
2.768AsnVal: 2.768 ± 0.422
0.725AsnTrp: 0.725 ± 0.228
0.791AsnTyr: 0.791 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
5.603ProAla: 5.603 ± 0.737
0.725ProCys: 0.725 ± 0.217
3.164ProAsp: 3.164 ± 0.486
3.296ProGlu: 3.296 ± 0.484
1.912ProPhe: 1.912 ± 0.345
4.548ProGly: 4.548 ± 0.553
0.791ProHis: 0.791 ± 0.219
2.768ProIle: 2.768 ± 0.398
3.23ProLys: 3.23 ± 0.476
3.23ProLeu: 3.23 ± 0.565
1.252ProMet: 1.252 ± 0.232
2.109ProAsn: 2.109 ± 0.33
2.505ProPro: 2.505 ± 0.504
2.9ProGln: 2.9 ± 1.114
3.164ProArg: 3.164 ± 0.445
2.966ProSer: 2.966 ± 0.553
4.219ProThr: 4.219 ± 0.595
3.23ProVal: 3.23 ± 0.492
1.582ProTrp: 1.582 ± 0.337
1.252ProTyr: 1.252 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
3.362GlnAla: 3.362 ± 0.675
0.264GlnCys: 0.264 ± 0.125
2.571GlnAsp: 2.571 ± 0.416
2.109GlnGlu: 2.109 ± 0.335
1.45GlnPhe: 1.45 ± 0.3
4.746GlnGly: 4.746 ± 2.305
0.725GlnHis: 0.725 ± 0.207
1.516GlnIle: 1.516 ± 0.362
1.318GlnLys: 1.318 ± 0.253
2.966GlnLeu: 2.966 ± 0.532
0.989GlnMet: 0.989 ± 0.345
0.791GlnAsn: 0.791 ± 0.245
1.714GlnPro: 1.714 ± 0.326
1.714GlnGln: 1.714 ± 0.308
2.966GlnArg: 2.966 ± 0.466
2.043GlnSer: 2.043 ± 0.384
1.318GlnThr: 1.318 ± 0.257
3.098GlnVal: 3.098 ± 0.379
0.857GlnTrp: 0.857 ± 0.23
1.648GlnTyr: 1.648 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
6.723ArgAla: 6.723 ± 0.741
0.725ArgCys: 0.725 ± 0.283
4.087ArgAsp: 4.087 ± 0.7
3.23ArgGlu: 3.23 ± 0.574
1.912ArgPhe: 1.912 ± 0.467
4.812ArgGly: 4.812 ± 0.668
1.516ArgHis: 1.516 ± 0.312
2.834ArgIle: 2.834 ± 0.465
4.548ArgLys: 4.548 ± 0.55
5.932ArgLeu: 5.932 ± 0.59
1.582ArgMet: 1.582 ± 0.32
2.768ArgAsn: 2.768 ± 0.449
2.571ArgPro: 2.571 ± 0.486
2.834ArgGln: 2.834 ± 0.397
6.196ArgArg: 6.196 ± 0.859
3.955ArgSer: 3.955 ± 0.439
2.9ArgThr: 2.9 ± 0.531
5.801ArgVal: 5.801 ± 0.602
1.45ArgTrp: 1.45 ± 0.396
1.648ArgTyr: 1.648 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
5.471SerAla: 5.471 ± 0.909
0.989SerCys: 0.989 ± 0.305
3.559SerAsp: 3.559 ± 0.511
2.834SerGlu: 2.834 ± 0.455
1.516SerPhe: 1.516 ± 0.298
5.01SerGly: 5.01 ± 0.772
1.384SerHis: 1.384 ± 0.315
2.637SerIle: 2.637 ± 0.436
2.307SerLys: 2.307 ± 0.394
4.482SerLeu: 4.482 ± 0.557
1.912SerMet: 1.912 ± 0.34
2.373SerAsn: 2.373 ± 0.367
3.362SerPro: 3.362 ± 0.577
1.582SerGln: 1.582 ± 0.331
3.164SerArg: 3.164 ± 0.361
3.428SerSer: 3.428 ± 0.416
3.032SerThr: 3.032 ± 0.445
2.505SerVal: 2.505 ± 0.401
1.582SerTrp: 1.582 ± 0.339
1.384SerTyr: 1.384 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
5.669ThrAla: 5.669 ± 0.667
0.527ThrCys: 0.527 ± 0.186
3.428ThrAsp: 3.428 ± 0.501
2.571ThrGlu: 2.571 ± 0.454
1.648ThrPhe: 1.648 ± 0.321
5.471ThrGly: 5.471 ± 0.716
1.055ThrHis: 1.055 ± 0.259
3.362ThrIle: 3.362 ± 0.686
2.966ThrLys: 2.966 ± 0.436
5.735ThrLeu: 5.735 ± 0.49
1.648ThrMet: 1.648 ± 0.415
1.977ThrAsn: 1.977 ± 0.299
4.087ThrPro: 4.087 ± 0.452
1.912ThrGln: 1.912 ± 0.331
4.35ThrArg: 4.35 ± 0.551
3.032ThrSer: 3.032 ± 0.456
4.087ThrThr: 4.087 ± 0.625
4.614ThrVal: 4.614 ± 0.576
1.45ThrTrp: 1.45 ± 0.331
1.384ThrTyr: 1.384 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.196ValAla: 6.196 ± 0.447
0.659ValCys: 0.659 ± 0.209
4.812ValAsp: 4.812 ± 0.531
4.68ValGlu: 4.68 ± 0.578
2.241ValPhe: 2.241 ± 0.315
4.482ValGly: 4.482 ± 0.608
1.252ValHis: 1.252 ± 0.273
3.955ValIle: 3.955 ± 0.46
2.966ValLys: 2.966 ± 0.41
6.064ValLeu: 6.064 ± 0.771
1.977ValMet: 1.977 ± 0.429
2.505ValAsn: 2.505 ± 0.416
3.823ValPro: 3.823 ± 0.488
2.768ValGln: 2.768 ± 0.414
3.625ValArg: 3.625 ± 0.407
3.23ValSer: 3.23 ± 0.513
5.471ValThr: 5.471 ± 0.572
6.394ValVal: 6.394 ± 0.771
1.648ValTrp: 1.648 ± 0.307
1.714ValTyr: 1.714 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
2.043TrpAla: 2.043 ± 0.425
0.395TrpCys: 0.395 ± 0.195
1.516TrpAsp: 1.516 ± 0.286
0.791TrpGlu: 0.791 ± 0.271
0.659TrpPhe: 0.659 ± 0.197
1.121TrpGly: 1.121 ± 0.316
0.527TrpHis: 0.527 ± 0.204
0.395TrpIle: 0.395 ± 0.124
0.857TrpLys: 0.857 ± 0.242
2.241TrpLeu: 2.241 ± 0.442
0.264TrpMet: 0.264 ± 0.12
1.846TrpAsn: 1.846 ± 0.327
0.923TrpPro: 0.923 ± 0.233
0.989TrpGln: 0.989 ± 0.299
2.043TrpArg: 2.043 ± 0.316
1.252TrpSer: 1.252 ± 0.285
1.186TrpThr: 1.186 ± 0.295
1.648TrpVal: 1.648 ± 0.386
0.461TrpTrp: 0.461 ± 0.177
0.791TrpTyr: 0.791 ± 0.216
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.109TyrAla: 2.109 ± 0.325
0.264TyrCys: 0.264 ± 0.11
2.043TyrAsp: 2.043 ± 0.398
2.043TyrGlu: 2.043 ± 0.353
0.989TyrPhe: 0.989 ± 0.222
2.307TyrGly: 2.307 ± 0.397
0.527TyrHis: 0.527 ± 0.197
1.384TyrIle: 1.384 ± 0.255
1.516TyrLys: 1.516 ± 0.412
2.241TyrLeu: 2.241 ± 0.399
1.186TyrMet: 1.186 ± 0.315
0.923TyrAsn: 0.923 ± 0.242
1.714TyrPro: 1.714 ± 0.294
0.989TyrGln: 0.989 ± 0.217
1.516TyrArg: 1.516 ± 0.342
1.582TyrSer: 1.582 ± 0.342
1.78TyrThr: 1.78 ± 0.321
1.912TyrVal: 1.912 ± 0.391
0.198TyrTrp: 0.198 ± 0.12
0.725TyrTyr: 0.725 ± 0.244
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (15172 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski