Gordonia phage YorkOnyx
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G8LMC3|A0A7G8LMC3_9CAUD Uncharacterized protein OS=Gordonia phage YorkOnyx OX=2762402 GN=74 PE=4 SV=1
MM1 pKa = 7.6 ASDD4 pKa = 5.73 DD5 pKa = 3.67 IRR7 pKa = 11.84 NYY9 pKa = 11.26 DD10 pKa = 3.6 PDD12 pKa = 3.03 TGMRR16 pKa = 11.84 YY17 pKa = 8.74 RR18 pKa = 11.84 WEE20 pKa = 4.16 MPDD23 pKa = 3.25 SEE25 pKa = 4.56 RR26 pKa = 11.84 GFVVEE31 pKa = 5.36 IDD33 pKa = 3.84 NDD35 pKa = 3.75 DD36 pKa = 3.92 TGPILCGCLCGPGYY50 pKa = 10.45 SRR52 pKa = 11.84 PAPFLYY58 pKa = 9.95 PDD60 pKa = 3.38 EE61 pKa = 4.68 ARR63 pKa = 11.84 WLAEE67 pKa = 4.07 HH68 pKa = 6.23 MPEE71 pKa = 3.54 MLAAIEE77 pKa = 4.56 AYY79 pKa = 9.48 EE80 pKa = 4.05 LQAGGASSS88 pKa = 3.2
Molecular weight: 9.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.806
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.77
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.795
Grimsley 3.681
Solomon 3.923
Lehninger 3.884
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.821
EMBOSS 3.872
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 3.961
Protein with the highest isoelectric point:
>tr|A0A7G8LM68|A0A7G8LM68_9CAUD HNH endonuclease OS=Gordonia phage YorkOnyx OX=2762402 GN=19 PE=4 SV=1
MM1 pKa = 7.16 GWLVFGPAFVVVRR14 pKa = 11.84 IVKK17 pKa = 9.78 IVEE20 pKa = 4.16 DD21 pKa = 3.64 ALSPKK26 pKa = 9.79 RR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 EE30 pKa = 4.14 LIRR33 pKa = 11.84 QQTRR37 pKa = 11.84 DD38 pKa = 3.48 EE39 pKa = 4.16 VAAAARR45 pKa = 11.84 RR46 pKa = 11.84 SEE48 pKa = 4.19 EE49 pKa = 3.8 YY50 pKa = 10.16 RR51 pKa = 11.84 RR52 pKa = 11.84 TRR54 pKa = 11.84 YY55 pKa = 10.12 GLL57 pKa = 3.49
Molecular weight: 6.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 9.867
IPC_protein 11.111
Toseland 11.155
ProMoST 11.535
Dawson 11.199
Bjellqvist 11.125
Wikipedia 11.608
Rodwell 11.008
Grimsley 11.242
Solomon 11.594
Lehninger 11.506
Nozaki 11.155
DTASelect 11.125
Thurlkill 11.155
EMBOSS 11.637
Sillero 11.169
Patrickios 10.804
IPC_peptide 11.594
IPC2_peptide 10.54
IPC2.peptide.svr19 9.138
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
18370
34
1672
218.7
23.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.471 ± 0.411
0.762 ± 0.096
7.643 ± 0.441
5.471 ± 0.394
2.373 ± 0.154
8.737 ± 0.452
2.09 ± 0.152
4.377 ± 0.272
2.379 ± 0.195
7.425 ± 0.263
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.111
2.597 ± 0.144
6.206 ± 0.268
3.745 ± 0.158
7.784 ± 0.454
4.878 ± 0.225
6.658 ± 0.278
7.98 ± 0.249
2.079 ± 0.131
2.216 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here