Pantoea alhagi
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3878 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6B2X0|A0A1W6B2X0_9GAMM 30S ribosomal protein S15 OS=Pantoea alhagi OX=1891675 GN=rpsO PE=3 SV=1
MM1 pKa = 7.73 AYY3 pKa = 10.03 EE4 pKa = 4.21 EE5 pKa = 4.33 LLEE8 pKa = 4.18 EE9 pKa = 4.2 QRR11 pKa = 11.84 EE12 pKa = 4.13 EE13 pKa = 3.55 TRR15 pKa = 11.84 AIIEE19 pKa = 4.17 EE20 pKa = 4.15 LLEE23 pKa = 5.17 DD24 pKa = 4.98 GSDD27 pKa = 3.46 PDD29 pKa = 3.46 ALYY32 pKa = 9.93 TIEE35 pKa = 4.84 HH36 pKa = 6.73 HH37 pKa = 6.7 LSCDD41 pKa = 3.18 SFEE44 pKa = 4.65 ALEE47 pKa = 4.28 KK48 pKa = 10.64 AAVDD52 pKa = 3.72 AFKK55 pKa = 10.84 LGYY58 pKa = 9.93 EE59 pKa = 4.03 VTEE62 pKa = 4.16 PEE64 pKa = 4.23 EE65 pKa = 5.31 LEE67 pKa = 4.98 LEE69 pKa = 4.62 DD70 pKa = 5.35 GSTVMCVDD78 pKa = 3.7 ILSEE82 pKa = 4.01 LALKK86 pKa = 10.62 PEE88 pKa = 4.36 LLDD91 pKa = 3.74 AQVEE95 pKa = 4.32 QLVNLAGKK103 pKa = 10.06 HH104 pKa = 4.6 NVDD107 pKa = 3.16 YY108 pKa = 10.89 DD109 pKa = 3.25 GWGTYY114 pKa = 10.12 FEE116 pKa = 5.91 DD117 pKa = 4.82 PDD119 pKa = 5.68 AEE121 pKa = 4.27 DD122 pKa = 4.38 EE123 pKa = 4.47 EE124 pKa = 4.66 EE125 pKa = 4.44 GDD127 pKa = 5.58 YY128 pKa = 10.76 IDD130 pKa = 5.62 EE131 pKa = 5.2 DD132 pKa = 4.56 DD133 pKa = 5.36 DD134 pKa = 5.38 GIRR137 pKa = 11.84 HH138 pKa = 6.01
Molecular weight: 15.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.694
IPC_protein 3.668
Toseland 3.478
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.541
Rodwell 3.503
Grimsley 3.389
Solomon 3.63
Lehninger 3.579
Nozaki 3.757
DTASelect 3.923
Thurlkill 3.516
EMBOSS 3.554
Sillero 3.783
Patrickios 1.812
IPC_peptide 3.63
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A1W6B4C5|A0A1W6B4C5_9GAMM LysR family transcriptional regulator OS=Pantoea alhagi OX=1891675 GN=B1H58_07605 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LTVSKK46 pKa = 11.03
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3878
0
3878
1212343
22
3779
312.6
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.147 ± 0.046
1.01 ± 0.015
5.033 ± 0.03
5.662 ± 0.043
3.76 ± 0.027
7.332 ± 0.042
2.284 ± 0.022
5.628 ± 0.033
3.901 ± 0.033
11.27 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.728 ± 0.019
3.649 ± 0.031
4.514 ± 0.03
4.976 ± 0.038
5.986 ± 0.04
5.94 ± 0.031
5.179 ± 0.028
6.768 ± 0.032
1.549 ± 0.02
2.683 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here