Roseisalinus antarcticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseisalinus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y5SNP9|A0A1Y5SNP9_9RHOB Pyridoxine 5'-phosphate synthase OS=Roseisalinus antarcticus OX=254357 GN=pdxJ PE=3 SV=1
MM1 pKa = 7.54TLRR4 pKa = 11.84ISLILGPVAACALALPAAAQDD25 pKa = 3.92YY26 pKa = 10.53CGGGSGTWIGGNQANSDD43 pKa = 3.38IATSDD48 pKa = 3.38TYY50 pKa = 11.02RR51 pKa = 11.84EE52 pKa = 3.84QLALVLLGNAHH63 pKa = 6.31VSLFDD68 pKa = 4.25LSSAMDD74 pKa = 3.45VRR76 pKa = 11.84LEE78 pKa = 3.97AQGRR82 pKa = 11.84GAGDD86 pKa = 3.53PVIEE90 pKa = 4.67ILDD93 pKa = 3.61STGAVVGGDD102 pKa = 3.74DD103 pKa = 4.53DD104 pKa = 5.24SGGGTSSAALLSLQPGTYY122 pKa = 10.59CMNTSSYY129 pKa = 11.87DD130 pKa = 3.79NAPLTATVRR139 pKa = 11.84IGQTRR144 pKa = 11.84HH145 pKa = 4.89EE146 pKa = 4.43ALTEE150 pKa = 4.21GGAQLSANGGNTGGGNTGGGDD171 pKa = 3.34TGNDD175 pKa = 3.63GGGSMTDD182 pKa = 3.06SGACGGAMMIGGGNPVEE199 pKa = 4.47GSLGGGLSVTNAIDD213 pKa = 3.84SVPTYY218 pKa = 10.51AFVVGSPTTLSITAEE233 pKa = 4.11NQDD236 pKa = 3.82ADD238 pKa = 3.99PVLTLTGDD246 pKa = 3.57DD247 pKa = 3.51GTFYY251 pKa = 11.4AEE253 pKa = 4.7NDD255 pKa = 3.88DD256 pKa = 4.81FDD258 pKa = 4.6GLNSRR263 pKa = 11.84IDD265 pKa = 3.54MTTALQPGTYY275 pKa = 9.86CIGLRR280 pKa = 11.84ALSNGTLPVTVALTGYY296 pKa = 10.4DD297 pKa = 3.55PVAAAQALYY306 pKa = 10.54DD307 pKa = 3.89QGEE310 pKa = 4.44VAPPMDD316 pKa = 3.98GSYY319 pKa = 10.67PITPLGTVASRR330 pKa = 11.84SVSNAQVQGDD340 pKa = 3.76RR341 pKa = 11.84AQWFSLTVDD350 pKa = 3.2QTGLLLIEE358 pKa = 5.92AIATSNGDD366 pKa = 3.28PVLRR370 pKa = 11.84LFDD373 pKa = 3.97DD374 pKa = 3.75VGRR377 pKa = 11.84QIGYY381 pKa = 10.04NDD383 pKa = 5.17DD384 pKa = 3.66YY385 pKa = 11.86GQGLDD390 pKa = 3.37SQIAARR396 pKa = 11.84VFPGTYY402 pKa = 10.02LVALTDD408 pKa = 3.87ISGSSPMLRR417 pKa = 11.84LVMEE421 pKa = 5.05RR422 pKa = 11.84YY423 pKa = 9.83VPAQQ427 pKa = 3.09

Molecular weight: 43.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y5TNX7|A0A1Y5TNX7_9RHOB Putative FAD-linked oxidoreductase OS=Roseisalinus antarcticus OX=254357 GN=ROA7023_03318 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84KK29 pKa = 8.8ILNARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.3KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4698

0

4698

1473940

29

12848

313.7

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.838 ± 0.06

0.839 ± 0.014

6.332 ± 0.062

5.793 ± 0.037

3.579 ± 0.024

9.189 ± 0.055

1.959 ± 0.024

4.916 ± 0.024

2.376 ± 0.037

10.016 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.031

2.295 ± 0.021

5.34 ± 0.036

2.884 ± 0.02

7.339 ± 0.047

5.118 ± 0.032

5.672 ± 0.048

7.382 ± 0.034

1.454 ± 0.017

2.093 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski