Amnimonas aquatica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Amnimonas

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1914 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6AUA2|A0A2P6AUA2_9GAMM RNA-binding protein Hfq OS=Amnimonas aquatica OX=2094561 GN=hfq PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.04KK3 pKa = 8.44WQCIVCGFVYY13 pKa = 10.58DD14 pKa = 4.38EE15 pKa = 4.46AVGMPWDD22 pKa = 5.36GIPAGTLWDD31 pKa = 5.95DD32 pKa = 4.27IPADD36 pKa = 4.24WVCPDD41 pKa = 4.0CGVGKK46 pKa = 10.44EE47 pKa = 4.02DD48 pKa = 4.52FEE50 pKa = 4.47MVEE53 pKa = 4.0II54 pKa = 4.86

Molecular weight: 6.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6AQN3|A0A2P6AQN3_9GAMM Dephospho-CoA kinase OS=Amnimonas aquatica OX=2094561 GN=coaE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.33QKK13 pKa = 9.06RR14 pKa = 11.84THH16 pKa = 6.13GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1914

0

1914

537966

23

1488

281.1

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.249 ± 0.078

0.909 ± 0.019

5.682 ± 0.045

5.636 ± 0.048

3.386 ± 0.035

8.442 ± 0.044

2.313 ± 0.027

4.563 ± 0.046

3.001 ± 0.053

11.4 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.024

2.438 ± 0.029

5.122 ± 0.045

3.996 ± 0.037

7.483 ± 0.056

5.105 ± 0.04

4.762 ± 0.033

7.524 ± 0.041

1.385 ± 0.025

2.225 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski