Methylovirgula ligni

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Methylovirgula

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9YUY7|A0A3D9YUY7_9RHIZ Uncharacterized protein OS=Methylovirgula ligni OX=569860 GN=DES32_2479 PE=4 SV=1
MM1 pKa = 7.37NLTTATHH8 pKa = 5.47VTQSALATVSAEE20 pKa = 3.94SALVSRR26 pKa = 11.84NIGGVNSPGFSEE38 pKa = 3.96KK39 pKa = 9.54TANVVTTADD48 pKa = 3.4GGVEE52 pKa = 4.17VASVTNAQSQAVFANVLSATAASATQSALSDD83 pKa = 3.69GLTTLATIVGEE94 pKa = 4.34PGSDD98 pKa = 2.94TSPAEE103 pKa = 4.08KK104 pKa = 10.64LSDD107 pKa = 3.63LTNALQQYY115 pKa = 7.24EE116 pKa = 4.42ASPSDD121 pKa = 3.42SSLASAAVTAGQNLASTLNNATATVQQVRR150 pKa = 11.84EE151 pKa = 4.15DD152 pKa = 4.24ADD154 pKa = 3.51SQMASSVSSINSLLTQFKK172 pKa = 10.56SVNQQIVTGTATGADD187 pKa = 3.69VTDD190 pKa = 4.95LLDD193 pKa = 3.62TRR195 pKa = 11.84NSILTQLSQQIGITTSAGANNDD217 pKa = 2.99MSIYY221 pKa = 8.91TDD223 pKa = 3.13SGVTLFQNGTASTVNFQPTQTYY245 pKa = 7.79TAGTTGNAVYY255 pKa = 10.27IDD257 pKa = 4.08GVPVTGSSATMPIQTGALAGLANLRR282 pKa = 11.84DD283 pKa = 4.23NVTVTYY289 pKa = 10.36QSQLDD294 pKa = 3.56QTANGLISAFAEE306 pKa = 4.05TDD308 pKa = 3.45PSNSGSSLAGLFTNGSSTALPTAAQVTGLAGSITVNAAVDD348 pKa = 3.99PSQGGTAALLSDD360 pKa = 5.22GINFNYY366 pKa = 7.85NTSNQASYY374 pKa = 8.62DD375 pKa = 3.88TQLQQYY381 pKa = 7.14LTKK384 pKa = 10.82LSAAQSFSSAGGIGTSNTLSGYY406 pKa = 10.2AAASVSWLDD415 pKa = 4.17AEE417 pKa = 4.36QQNVQSEE424 pKa = 4.52SAYY427 pKa = 10.69QSTLLSTSTTALSNSTGVNLDD448 pKa = 4.09DD449 pKa = 5.8EE450 pKa = 4.79MSQMLDD456 pKa = 3.4LEE458 pKa = 4.4NSYY461 pKa = 11.36SATAKK466 pKa = 10.81LLTTINNMFSDD477 pKa = 4.72LATAIDD483 pKa = 3.77QATAAA488 pKa = 4.49

Molecular weight: 49.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9YYI5|A0A3D9YYI5_9RHIZ GNAT family acetyltransferase OS=Methylovirgula ligni OX=569860 GN=DES32_1057 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.36VIATRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.01RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3170

0

3170

974626

26

1985

307.5

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.216 ± 0.072

0.807 ± 0.013

5.563 ± 0.034

5.491 ± 0.04

3.935 ± 0.026

8.169 ± 0.041

2.175 ± 0.021

5.45 ± 0.031

3.536 ± 0.035

10.384 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.019

2.657 ± 0.028

5.374 ± 0.038

3.129 ± 0.029

6.844 ± 0.043

5.374 ± 0.033

5.236 ± 0.034

7.135 ± 0.038

1.239 ± 0.018

2.341 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski