Streptomyces luteoverticillatus (Streptoverticillium luteoverticillatus)
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9G105|A0A3Q9G105_STRLT Acyl-CoA dehydrogenase OS=Streptomyces luteoverticillatus OX=66425 GN=EKH77_19120 PE=3 SV=1
MM1 pKa = 7.82 LSFEE5 pKa = 4.29 QAAEE9 pKa = 3.85 LARR12 pKa = 11.84 IFLAEE17 pKa = 4.01 KK18 pKa = 10.24 SRR20 pKa = 11.84 YY21 pKa = 7.78 STLPMDD27 pKa = 4.59 IVEE30 pKa = 4.66 EE31 pKa = 4.04 DD32 pKa = 3.16 HH33 pKa = 7.25 ALLDD37 pKa = 3.6 GKK39 pKa = 10.56 IYY41 pKa = 10.52 FDD43 pKa = 4.23 CQSIAYY49 pKa = 8.33 LKK51 pKa = 10.7 SGNPSDD57 pKa = 3.89 MVIGTGYY64 pKa = 9.9 ICVDD68 pKa = 4.13 GEE70 pKa = 4.39 TGDD73 pKa = 4.01 CRR75 pKa = 11.84 MLGAVEE81 pKa = 4.48 SVEE84 pKa = 4.68 LNLFEE89 pKa = 7.33 DD90 pKa = 4.75 DD91 pKa = 5.04 DD92 pKa = 4.88 FPGAPEE98 pKa = 3.61
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.656
ProMoST 3.973
Dawson 3.834
Bjellqvist 4.05
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.579
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A3Q9G0C3|A0A3Q9G0C3_STRLT Uncharacterized protein OS=Streptomyces luteoverticillatus OX=66425 GN=EKH77_27885 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.36 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6118
0
6118
2059982
29
9511
336.7
35.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.936 ± 0.053
0.832 ± 0.009
5.882 ± 0.027
5.689 ± 0.036
2.724 ± 0.018
9.727 ± 0.033
2.352 ± 0.016
2.982 ± 0.023
2.208 ± 0.029
10.276 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.721 ± 0.013
1.708 ± 0.017
6.269 ± 0.031
2.506 ± 0.02
8.373 ± 0.036
4.845 ± 0.024
6.045 ± 0.03
8.398 ± 0.03
1.508 ± 0.013
2.018 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here