Streptomyces sp. SID13726

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9812 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2XH38|A0A6B2XH38_9ACTN Response regulator transcription factor OS=Streptomyces sp. SID13726 OX=2706058 GN=G3I78_33415 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 5.18RR3 pKa = 11.84YY4 pKa = 10.54RR5 pKa = 11.84RR6 pKa = 11.84GWTAAAVCGVLMLGGCSAGGDD27 pKa = 3.57GDD29 pKa = 4.73SPFGDD34 pKa = 3.27VSSSSEE40 pKa = 3.96EE41 pKa = 4.02TGVEE45 pKa = 4.2PTRR48 pKa = 11.84SSPDD52 pKa = 3.47DD53 pKa = 3.72VPDD56 pKa = 5.26GDD58 pKa = 4.58VGWQAEE64 pKa = 4.47TEE66 pKa = 4.2DD67 pKa = 3.76AVAVVDD73 pKa = 4.28TFWATHH79 pKa = 5.68WNDD82 pKa = 3.41HH83 pKa = 4.99FTGAYY88 pKa = 7.93EE89 pKa = 4.15SPQVFGAYY97 pKa = 9.7RR98 pKa = 11.84AGSPDD103 pKa = 3.24APGCGGEE110 pKa = 4.1PALPGNAFYY119 pKa = 9.56CTDD122 pKa = 3.77GDD124 pKa = 4.02FLAWDD129 pKa = 4.13AQLMADD135 pKa = 4.99GYY137 pKa = 10.95RR138 pKa = 11.84SGDD141 pKa = 2.93SWVYY145 pKa = 10.45LVVAHH150 pKa = 6.36EE151 pKa = 4.5WGHH154 pKa = 5.7AVQNRR159 pKa = 11.84VLGLSDD165 pKa = 3.52VAQEE169 pKa = 4.1LQADD173 pKa = 4.44CLAGATLFGSEE184 pKa = 4.46DD185 pKa = 3.39LRR187 pKa = 11.84FEE189 pKa = 4.77EE190 pKa = 5.56GDD192 pKa = 3.57TDD194 pKa = 3.79EE195 pKa = 5.39LAAALAALADD205 pKa = 3.99DD206 pKa = 4.6TPWTNSSDD214 pKa = 3.47HH215 pKa = 6.66GDD217 pKa = 3.38ANEE220 pKa = 4.84RR221 pKa = 11.84ISSFAAGGRR230 pKa = 11.84GGVEE234 pKa = 3.16ACMPTEE240 pKa = 3.77

Molecular weight: 25.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2XFU2|A0A6B2XFU2_9ACTN Uncharacterized protein OS=Streptomyces sp. SID13726 OX=2706058 GN=G3I78_46660 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9812

0

9812

3164316

18

11988

322.5

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.239 ± 0.04

0.763 ± 0.006

6.081 ± 0.019

5.687 ± 0.025

2.778 ± 0.015

9.406 ± 0.026

2.301 ± 0.013

3.099 ± 0.019

2.196 ± 0.023

10.444 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.01

1.827 ± 0.014

5.934 ± 0.022

2.756 ± 0.015

7.858 ± 0.026

5.235 ± 0.024

6.382 ± 0.024

8.58 ± 0.024

1.577 ± 0.011

2.176 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski