Amino acid dipepetide frequency for Streptomyces sp. SID13726

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.939AlaAla: 19.939 ± 0.114
1.04AlaCys: 1.04 ± 0.023
8.309AlaAsp: 8.309 ± 0.059
8.598AlaGlu: 8.598 ± 0.064
3.509AlaPhe: 3.509 ± 0.039
12.54AlaGly: 12.54 ± 0.074
2.879AlaHis: 2.879 ± 0.038
3.337AlaIle: 3.337 ± 0.039
2.854AlaLys: 2.854 ± 0.038
14.549AlaLeu: 14.549 ± 0.101
2.326AlaMet: 2.326 ± 0.029
2.001AlaAsn: 2.001 ± 0.025
6.693AlaPro: 6.693 ± 0.058
3.736AlaGln: 3.736 ± 0.041
9.971AlaArg: 9.971 ± 0.069
5.97AlaSer: 5.97 ± 0.048
7.152AlaThr: 7.152 ± 0.049
12.305AlaVal: 12.305 ± 0.075
1.862AlaTrp: 1.862 ± 0.024
2.819AlaTyr: 2.819 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.062CysAla: 1.062 ± 0.02
0.089CysCys: 0.089 ± 0.006
0.452CysAsp: 0.452 ± 0.012
0.427CysGlu: 0.427 ± 0.013
0.21CysPhe: 0.21 ± 0.008
0.883CysGly: 0.883 ± 0.022
0.193CysHis: 0.193 ± 0.009
0.148CysIle: 0.148 ± 0.007
0.114CysLys: 0.114 ± 0.007
0.744CysLeu: 0.744 ± 0.016
0.118CysMet: 0.118 ± 0.006
0.132CysAsn: 0.132 ± 0.007
0.448CysPro: 0.448 ± 0.012
0.163CysGln: 0.163 ± 0.007
0.574CysArg: 0.574 ± 0.015
0.425CysSer: 0.425 ± 0.013
0.507CysThr: 0.507 ± 0.013
0.668CysVal: 0.668 ± 0.014
0.128CysTrp: 0.128 ± 0.007
0.146CysTyr: 0.146 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.402AspAla: 7.402 ± 0.057
0.403AspCys: 0.403 ± 0.012
3.66AspAsp: 3.66 ± 0.04
3.872AspGlu: 3.872 ± 0.041
1.738AspPhe: 1.738 ± 0.024
6.525AspGly: 6.525 ± 0.056
1.43AspHis: 1.43 ± 0.019
1.895AspIle: 1.895 ± 0.023
1.217AspLys: 1.217 ± 0.021
6.434AspLeu: 6.434 ± 0.051
0.827AspMet: 0.827 ± 0.017
1.022AspAsn: 1.022 ± 0.019
4.538AspPro: 4.538 ± 0.042
1.582AspGln: 1.582 ± 0.025
4.891AspArg: 4.891 ± 0.04
2.81AspSer: 2.81 ± 0.03
3.454AspThr: 3.454 ± 0.036
4.839AspVal: 4.839 ± 0.047
1.116AspTrp: 1.116 ± 0.02
1.159AspTyr: 1.159 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.167GluAla: 7.167 ± 0.054
0.358GluCys: 0.358 ± 0.011
2.83GluAsp: 2.83 ± 0.039
3.556GluGlu: 3.556 ± 0.05
1.549GluPhe: 1.549 ± 0.021
4.367GluGly: 4.367 ± 0.039
1.529GluHis: 1.529 ± 0.024
2.266GluIle: 2.266 ± 0.026
1.451GluLys: 1.451 ± 0.026
6.892GluLeu: 6.892 ± 0.061
0.851GluMet: 0.851 ± 0.017
1.046GluAsn: 1.046 ± 0.019
3.338GluPro: 3.338 ± 0.034
2.204GluGln: 2.204 ± 0.029
5.322GluArg: 5.322 ± 0.047
2.709GluSer: 2.709 ± 0.031
3.03GluThr: 3.03 ± 0.034
4.448GluVal: 4.448 ± 0.047
0.814GluTrp: 0.814 ± 0.017
1.147GluTyr: 1.147 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.649PheAla: 3.649 ± 0.035
0.257PheCys: 0.257 ± 0.01
1.986PheAsp: 1.986 ± 0.026
1.494PheGlu: 1.494 ± 0.026
0.881PhePhe: 0.881 ± 0.021
3.067PheGly: 3.067 ± 0.034
0.633PheHis: 0.633 ± 0.014
0.683PheIle: 0.683 ± 0.017
0.551PheLys: 0.551 ± 0.013
2.655PheLeu: 2.655 ± 0.032
0.412PheMet: 0.412 ± 0.011
0.586PheAsn: 0.586 ± 0.015
1.393PhePro: 1.393 ± 0.022
0.715PheGln: 0.715 ± 0.015
1.836PheArg: 1.836 ± 0.025
1.502PheSer: 1.502 ± 0.021
2.102PheThr: 2.102 ± 0.027
2.32PheVal: 2.32 ± 0.027
0.44PheTrp: 0.44 ± 0.014
0.621PheTyr: 0.621 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
10.856GlyAla: 10.856 ± 0.066
0.788GlyCys: 0.788 ± 0.017
5.247GlyAsp: 5.247 ± 0.04
5.037GlyGlu: 5.037 ± 0.045
2.949GlyPhe: 2.949 ± 0.034
8.609GlyGly: 8.609 ± 0.075
2.309GlyHis: 2.309 ± 0.029
3.44GlyIle: 3.44 ± 0.031
2.413GlyLys: 2.413 ± 0.036
9.475GlyLeu: 9.475 ± 0.062
1.903GlyMet: 1.903 ± 0.025
1.796GlyAsn: 1.796 ± 0.029
4.978GlyPro: 4.978 ± 0.051
2.686GlyGln: 2.686 ± 0.032
7.502GlyArg: 7.502 ± 0.055
5.506GlySer: 5.506 ± 0.047
6.777GlyThr: 6.777 ± 0.055
7.72GlyVal: 7.72 ± 0.061
1.731GlyTrp: 1.731 ± 0.03
2.339GlyTyr: 2.339 ± 0.03
0.0GlyXaa: 0.0 ± 0.0
His
2.654HisAla: 2.654 ± 0.034
0.207HisCys: 0.207 ± 0.007
1.363HisAsp: 1.363 ± 0.024
1.202HisGlu: 1.202 ± 0.023
0.661HisPhe: 0.661 ± 0.014
2.37HisGly: 2.37 ± 0.029
0.701HisHis: 0.701 ± 0.018
0.684HisIle: 0.684 ± 0.017
0.367HisLys: 0.367 ± 0.012
2.435HisLeu: 2.435 ± 0.037
0.321HisMet: 0.321 ± 0.01
0.37HisAsn: 0.37 ± 0.012
1.813HisPro: 1.813 ± 0.023
0.647HisGln: 0.647 ± 0.015
2.136HisArg: 2.136 ± 0.029
1.048HisSer: 1.048 ± 0.018
1.392HisThr: 1.392 ± 0.021
1.73HisVal: 1.73 ± 0.026
0.394HisTrp: 0.394 ± 0.011
0.509HisTyr: 0.509 ± 0.012
0.0HisXaa: 0.0 ± 0.0
Ile
4.658IleAla: 4.658 ± 0.04
0.272IleCys: 0.272 ± 0.01
2.181IleAsp: 2.181 ± 0.032
1.881IleGlu: 1.881 ± 0.026
0.683IlePhe: 0.683 ± 0.015
3.476IleGly: 3.476 ± 0.036
0.612IleHis: 0.612 ± 0.014
0.808IleIle: 0.808 ± 0.019
0.714IleLys: 0.714 ± 0.018
2.407IleLeu: 2.407 ± 0.033
0.447IleMet: 0.447 ± 0.012
0.687IleAsn: 0.687 ± 0.016
1.764IlePro: 1.764 ± 0.026
0.732IleGln: 0.732 ± 0.016
2.225IleArg: 2.225 ± 0.026
1.649IleSer: 1.649 ± 0.02
2.21IleThr: 2.21 ± 0.027
2.668IleVal: 2.668 ± 0.032
0.398IleTrp: 0.398 ± 0.01
0.524IleTyr: 0.524 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
3.015LysAla: 3.015 ± 0.041
0.113LysCys: 0.113 ± 0.007
1.389LysAsp: 1.389 ± 0.023
1.2LysGlu: 1.2 ± 0.025
0.47LysPhe: 0.47 ± 0.013
1.885LysGly: 1.885 ± 0.028
0.422LysHis: 0.422 ± 0.012
0.823LysIle: 0.823 ± 0.019
0.874LysLys: 0.874 ± 0.023
2.016LysLeu: 2.016 ± 0.032
0.379LysMet: 0.379 ± 0.011
0.545LysAsn: 0.545 ± 0.015
1.315LysPro: 1.315 ± 0.024
0.703LysGln: 0.703 ± 0.017
1.432LysArg: 1.432 ± 0.027
1.262LysSer: 1.262 ± 0.023
1.296LysThr: 1.296 ± 0.024
2.006LysVal: 2.006 ± 0.028
0.313LysTrp: 0.313 ± 0.011
0.502LysTyr: 0.502 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
15.199LeuAla: 15.199 ± 0.094
0.846LeuCys: 0.846 ± 0.019
6.856LeuAsp: 6.856 ± 0.055
4.732LeuGlu: 4.732 ± 0.046
2.656LeuPhe: 2.656 ± 0.035
9.337LeuGly: 9.337 ± 0.064
2.335LeuHis: 2.335 ± 0.031
3.212LeuIle: 3.212 ± 0.037
2.178LeuLys: 2.178 ± 0.03
11.482LeuLeu: 11.482 ± 0.092
1.63LeuMet: 1.63 ± 0.027
1.739LeuAsn: 1.739 ± 0.023
6.494LeuPro: 6.494 ± 0.049
2.19LeuGln: 2.19 ± 0.028
8.534LeuArg: 8.534 ± 0.066
5.534LeuSer: 5.534 ± 0.048
7.221LeuThr: 7.221 ± 0.051
8.93LeuVal: 8.93 ± 0.069
1.378LeuTrp: 1.378 ± 0.022
1.956LeuTyr: 1.956 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.157MetAla: 2.157 ± 0.03
0.133MetCys: 0.133 ± 0.007
0.886MetAsp: 0.886 ± 0.018
0.7MetGlu: 0.7 ± 0.016
0.453MetPhe: 0.453 ± 0.013
1.295MetGly: 1.295 ± 0.021
0.336MetHis: 0.336 ± 0.01
0.642MetIle: 0.642 ± 0.014
0.421MetLys: 0.421 ± 0.012
1.599MetLeu: 1.599 ± 0.026
0.278MetMet: 0.278 ± 0.01
0.432MetAsn: 0.432 ± 0.012
1.037MetPro: 1.037 ± 0.02
0.398MetGln: 0.398 ± 0.01
1.337MetArg: 1.337 ± 0.021
1.34MetSer: 1.34 ± 0.021
1.555MetThr: 1.555 ± 0.019
1.279MetVal: 1.279 ± 0.022
0.21MetTrp: 0.21 ± 0.009
0.32MetTyr: 0.32 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.208AsnAla: 2.208 ± 0.03
0.17AsnCys: 0.17 ± 0.008
1.011AsnAsp: 1.011 ± 0.019
0.782AsnGlu: 0.782 ± 0.016
0.497AsnPhe: 0.497 ± 0.011
1.968AsnGly: 1.968 ± 0.031
0.406AsnHis: 0.406 ± 0.011
0.67AsnIle: 0.67 ± 0.015
0.429AsnLys: 0.429 ± 0.012
1.67AsnLeu: 1.67 ± 0.024
0.286AsnMet: 0.286 ± 0.01
0.461AsnAsn: 0.461 ± 0.018
1.382AsnPro: 1.382 ± 0.021
0.533AsnGln: 0.533 ± 0.013
1.279AsnArg: 1.279 ± 0.022
1.101AsnSer: 1.101 ± 0.023
1.245AsnThr: 1.245 ± 0.024
1.394AsnVal: 1.394 ± 0.021
0.318AsnTrp: 0.318 ± 0.011
0.456AsnTyr: 0.456 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.118ProAla: 8.118 ± 0.065
0.312ProCys: 0.312 ± 0.011
4.48ProAsp: 4.48 ± 0.041
4.363ProGlu: 4.363 ± 0.04
1.554ProPhe: 1.554 ± 0.022
6.315ProGly: 6.315 ± 0.065
1.39ProHis: 1.39 ± 0.023
1.229ProIle: 1.229 ± 0.018
1.168ProLys: 1.168 ± 0.021
5.361ProLeu: 5.361 ± 0.048
0.952ProMet: 0.952 ± 0.016
0.922ProAsn: 0.922 ± 0.017
3.236ProPro: 3.236 ± 0.056
1.659ProGln: 1.659 ± 0.028
3.847ProArg: 3.847 ± 0.04
3.234ProSer: 3.234 ± 0.04
3.362ProThr: 3.362 ± 0.034
5.505ProVal: 5.505 ± 0.045
0.9ProTrp: 0.9 ± 0.018
1.433ProTyr: 1.433 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.582GlnAla: 3.582 ± 0.041
0.172GlnCys: 0.172 ± 0.007
1.493GlnAsp: 1.493 ± 0.022
1.462GlnGlu: 1.462 ± 0.021
0.702GlnPhe: 0.702 ± 0.018
2.365GlnGly: 2.365 ± 0.029
0.667GlnHis: 0.667 ± 0.014
1.069GlnIle: 1.069 ± 0.02
0.618GlnLys: 0.618 ± 0.016
3.134GlnLeu: 3.134 ± 0.035
0.481GlnMet: 0.481 ± 0.013
0.524GlnAsn: 0.524 ± 0.014
1.62GlnPro: 1.62 ± 0.028
1.215GlnGln: 1.215 ± 0.025
2.282GlnArg: 2.282 ± 0.029
1.307GlnSer: 1.307 ± 0.019
1.356GlnThr: 1.356 ± 0.026
2.365GlnVal: 2.365 ± 0.029
0.502GlnTrp: 0.502 ± 0.014
0.636GlnTyr: 0.636 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.449ArgAla: 9.449 ± 0.063
0.568ArgCys: 0.568 ± 0.013
4.243ArgAsp: 4.243 ± 0.041
4.745ArgGlu: 4.745 ± 0.041
2.333ArgPhe: 2.333 ± 0.027
5.673ArgGly: 5.673 ± 0.043
2.137ArgHis: 2.137 ± 0.028
3.112ArgIle: 3.112 ± 0.034
1.626ArgLys: 1.626 ± 0.026
8.882ArgLeu: 8.882 ± 0.074
1.631ArgMet: 1.631 ± 0.022
1.318ArgAsn: 1.318 ± 0.021
4.732ArgPro: 4.732 ± 0.05
2.271ArgGln: 2.271 ± 0.029
7.359ArgArg: 7.359 ± 0.062
3.93ArgSer: 3.93 ± 0.04
5.506ArgThr: 5.506 ± 0.042
5.916ArgVal: 5.916 ± 0.041
1.341ArgTrp: 1.341 ± 0.02
1.81ArgTyr: 1.81 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.915SerAla: 6.915 ± 0.05
0.421SerCys: 0.421 ± 0.012
2.871SerAsp: 2.871 ± 0.03
2.502SerGlu: 2.502 ± 0.026
1.572SerPhe: 1.572 ± 0.023
6.208SerGly: 6.208 ± 0.046
1.031SerHis: 1.031 ± 0.017
1.436SerIle: 1.436 ± 0.024
1.106SerLys: 1.106 ± 0.02
5.034SerLeu: 5.034 ± 0.045
1.056SerMet: 1.056 ± 0.019
0.96SerAsn: 0.96 ± 0.018
3.192SerPro: 3.192 ± 0.038
1.318SerGln: 1.318 ± 0.024
3.666SerArg: 3.666 ± 0.033
3.009SerSer: 3.009 ± 0.04
3.221SerThr: 3.221 ± 0.041
4.476SerVal: 4.476 ± 0.041
0.992SerTrp: 0.992 ± 0.018
1.368SerTyr: 1.368 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.292ThrAla: 9.292 ± 0.069
0.421ThrCys: 0.421 ± 0.012
3.834ThrAsp: 3.834 ± 0.034
3.428ThrGlu: 3.428 ± 0.035
1.688ThrPhe: 1.688 ± 0.023
6.965ThrGly: 6.965 ± 0.053
1.247ThrHis: 1.247 ± 0.021
1.679ThrIle: 1.679 ± 0.024
1.244ThrLys: 1.244 ± 0.024
6.026ThrLeu: 6.026 ± 0.048
0.917ThrMet: 0.917 ± 0.018
1.105ThrAsn: 1.105 ± 0.02
4.258ThrPro: 4.258 ± 0.042
1.398ThrGln: 1.398 ± 0.021
3.991ThrArg: 3.991 ± 0.035
3.415ThrSer: 3.415 ± 0.037
4.12ThrThr: 4.12 ± 0.055
6.348ThrVal: 6.348 ± 0.051
0.969ThrTrp: 0.969 ± 0.02
1.479ThrTyr: 1.479 ± 0.023
0.0ThrXaa: 0.0 ± 0.0
Val
10.596ValAla: 10.596 ± 0.066
0.737ValCys: 0.737 ± 0.015
5.1ValAsp: 5.1 ± 0.035
4.871ValGlu: 4.871 ± 0.042
2.459ValPhe: 2.459 ± 0.032
6.633ValGly: 6.633 ± 0.054
1.977ValHis: 1.977 ± 0.028
2.788ValIle: 2.788 ± 0.032
1.801ValLys: 1.801 ± 0.026
9.662ValLeu: 9.662 ± 0.071
1.406ValMet: 1.406 ± 0.025
1.714ValAsn: 1.714 ± 0.025
5.226ValPro: 5.226 ± 0.042
2.134ValGln: 2.134 ± 0.026
7.228ValArg: 7.228 ± 0.056
4.534ValSer: 4.534 ± 0.041
5.904ValThr: 5.904 ± 0.046
8.146ValVal: 8.146 ± 0.069
1.229ValTrp: 1.229 ± 0.021
1.648ValTyr: 1.648 ± 0.025
0.0ValXaa: 0.0 ± 0.0
Trp
1.698TrpAla: 1.698 ± 0.025
0.147TrpCys: 0.147 ± 0.007
0.903TrpAsp: 0.903 ± 0.019
0.746TrpGlu: 0.746 ± 0.017
0.521TrpPhe: 0.521 ± 0.014
1.127TrpGly: 1.127 ± 0.02
0.387TrpHis: 0.387 ± 0.011
0.574TrpIle: 0.574 ± 0.014
0.391TrpLys: 0.391 ± 0.011
1.806TrpLeu: 1.806 ± 0.026
0.307TrpMet: 0.307 ± 0.011
0.457TrpAsn: 0.457 ± 0.014
0.815TrpPro: 0.815 ± 0.017
0.644TrpGln: 0.644 ± 0.015
1.36TrpArg: 1.36 ± 0.019
1.011TrpSer: 1.011 ± 0.019
1.135TrpThr: 1.135 ± 0.02
0.995TrpVal: 0.995 ± 0.019
0.349TrpTrp: 0.349 ± 0.011
0.393TrpTyr: 0.393 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.772TyrAla: 2.772 ± 0.028
0.177TyrCys: 0.177 ± 0.009
1.721TyrAsp: 1.721 ± 0.028
1.272TyrGlu: 1.272 ± 0.023
0.7TyrPhe: 0.7 ± 0.015
2.453TyrGly: 2.453 ± 0.034
0.385TyrHis: 0.385 ± 0.011
0.5TyrIle: 0.5 ± 0.013
0.421TyrLys: 0.421 ± 0.012
2.175TyrLeu: 2.175 ± 0.03
0.276TyrMet: 0.276 ± 0.011
0.449TyrAsn: 0.449 ± 0.016
1.066TyrPro: 1.066 ± 0.018
0.629TyrGln: 0.629 ± 0.013
1.812TyrArg: 1.812 ± 0.026
1.057TyrSer: 1.057 ± 0.02
1.279TyrThr: 1.279 ± 0.023
1.738TyrVal: 1.738 ± 0.022
0.385TyrTrp: 0.385 ± 0.012
0.495TyrTyr: 0.495 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9812 proteins (3164316 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski