Synechococcus phage S-H34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 246 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R752|A0A6G8R752_9CAUD Uncharacterized protein OS=Synechococcus phage S-H34 OX=2718942 PE=4 SV=1
MM1 pKa = 7.33FPSQSFLQSFKK12 pKa = 11.34APTLAEE18 pKa = 4.17LLQMYY23 pKa = 8.56QYY25 pKa = 11.16RR26 pKa = 11.84SGSAHH31 pKa = 7.3AIEE34 pKa = 4.84KK35 pKa = 10.45LAVQQTLNARR45 pKa = 11.84GNEE48 pKa = 4.05LVDD51 pKa = 3.42MSAALQQITTAIDD64 pKa = 3.42AGQTAVSVTVPVQISEE80 pKa = 4.36TVEE83 pKa = 3.9LDD85 pKa = 3.24VTVNYY90 pKa = 10.11EE91 pKa = 3.89LDD93 pKa = 3.07VDD95 pKa = 3.98YY96 pKa = 10.45TLEE99 pKa = 4.08VNISNNVAVDD109 pKa = 3.19IDD111 pKa = 4.15FEE113 pKa = 4.69GVNINDD119 pKa = 3.57QLDD122 pKa = 4.01TFTVQADD129 pKa = 4.29FAQDD133 pKa = 3.3SRR135 pKa = 11.84VSDD138 pKa = 3.57LRR140 pKa = 11.84HH141 pKa = 6.59DD142 pKa = 3.88YY143 pKa = 11.08VRR145 pKa = 11.84GQGYY149 pKa = 10.16DD150 pKa = 3.34STHH153 pKa = 5.82GNFWFRR159 pKa = 11.84VEE161 pKa = 5.45DD162 pKa = 3.97DD163 pKa = 4.05SEE165 pKa = 4.62NNIPIPYY172 pKa = 9.7SGGQFYY178 pKa = 10.6PNGFEE183 pKa = 4.28EE184 pKa = 4.92GGEE187 pKa = 3.99YY188 pKa = 10.9AFFAVEE194 pKa = 4.17KK195 pKa = 9.55GRR197 pKa = 11.84HH198 pKa = 4.89GLSVGDD204 pKa = 3.61QLTNYY209 pKa = 8.85SGPMAEE215 pKa = 3.97AQARR219 pKa = 11.84YY220 pKa = 9.64IGGDD224 pKa = 3.22QSSDD228 pKa = 2.86VAYY231 pKa = 10.58ANNFDD236 pKa = 4.59ADD238 pKa = 4.12EE239 pKa = 4.83LLAADD244 pKa = 4.5PGFLNGFTLATVDD257 pKa = 3.44MGTGEE262 pKa = 4.38VLSLLNQHH270 pKa = 5.78NHH272 pKa = 3.97STNWANALADD282 pKa = 3.8QATQDD287 pKa = 3.27IQVII291 pKa = 4.03

Molecular weight: 31.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R6E2|A0A6G8R6E2_9CAUD Uncharacterized protein OS=Synechococcus phage S-H34 OX=2718942 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 5.3KK3 pKa = 11.22GGLPASLLLGSPTSRR18 pKa = 11.84LARR21 pKa = 11.84FSSRR25 pKa = 11.84TDD27 pKa = 2.83WAGTVPADD35 pKa = 3.35TAEE38 pKa = 4.25RR39 pKa = 11.84LGSSSRR45 pKa = 11.84VV46 pKa = 3.26

Molecular weight: 4.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

246

0

246

52067

30

2477

211.7

23.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.784 ± 0.226

0.862 ± 0.079

6.618 ± 0.14

6.724 ± 0.244

4.031 ± 0.111

7.823 ± 0.251

1.611 ± 0.089

5.237 ± 0.155

5.746 ± 0.388

7.548 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.125

5.138 ± 0.164

4.093 ± 0.128

3.893 ± 0.105

4.435 ± 0.147

6.834 ± 0.235

7.152 ± 0.357

6.739 ± 0.185

1.323 ± 0.076

4.1 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski