Amino acid dipepetide frequency for Synechococcus phage S-H34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.49AlaAla: 7.49 ± 0.566
0.576AlaCys: 0.576 ± 0.112
5.243AlaAsp: 5.243 ± 0.349
5.013AlaGlu: 5.013 ± 0.353
2.823AlaPhe: 2.823 ± 0.239
6.914AlaGly: 6.914 ± 0.473
1.095AlaHis: 1.095 ± 0.154
4.264AlaIle: 4.264 ± 0.269
4.686AlaLys: 4.686 ± 0.454
5.877AlaLeu: 5.877 ± 0.399
1.613AlaMet: 1.613 ± 0.199
3.764AlaAsn: 3.764 ± 0.274
3.111AlaPro: 3.111 ± 0.282
2.785AlaGln: 2.785 ± 0.218
3.227AlaArg: 3.227 ± 0.251
5.224AlaSer: 5.224 ± 0.35
5.57AlaThr: 5.57 ± 0.396
5.435AlaVal: 5.435 ± 0.341
0.749AlaTrp: 0.749 ± 0.128
2.382AlaTyr: 2.382 ± 0.185
0.0AlaXaa: 0.0 ± 0.0
Cys
0.346CysAla: 0.346 ± 0.098
0.077CysCys: 0.077 ± 0.036
0.634CysAsp: 0.634 ± 0.127
0.883CysGlu: 0.883 ± 0.15
0.327CysPhe: 0.327 ± 0.074
0.519CysGly: 0.519 ± 0.115
0.269CysHis: 0.269 ± 0.072
0.403CysIle: 0.403 ± 0.083
0.403CysLys: 0.403 ± 0.096
0.557CysLeu: 0.557 ± 0.125
0.211CysMet: 0.211 ± 0.067
0.365CysAsn: 0.365 ± 0.096
0.423CysPro: 0.423 ± 0.104
0.365CysGln: 0.365 ± 0.079
0.346CysArg: 0.346 ± 0.098
0.768CysSer: 0.768 ± 0.114
0.48CysThr: 0.48 ± 0.087
0.749CysVal: 0.749 ± 0.13
0.192CysTrp: 0.192 ± 0.06
0.307CysTyr: 0.307 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
5.263AspAla: 5.263 ± 0.323
0.499AspCys: 0.499 ± 0.11
4.187AspAsp: 4.187 ± 0.306
4.091AspGlu: 4.091 ± 0.338
3.015AspPhe: 3.015 ± 0.235
5.781AspGly: 5.781 ± 0.513
1.018AspHis: 1.018 ± 0.137
3.534AspIle: 3.534 ± 0.22
3.611AspLys: 3.611 ± 0.353
5.627AspLeu: 5.627 ± 0.411
1.344AspMet: 1.344 ± 0.189
2.785AspAsn: 2.785 ± 0.24
3.188AspPro: 3.188 ± 0.314
2.766AspGln: 2.766 ± 0.233
2.823AspArg: 2.823 ± 0.233
4.014AspSer: 4.014 ± 0.355
4.149AspThr: 4.149 ± 0.273
4.437AspVal: 4.437 ± 0.3
0.941AspTrp: 0.941 ± 0.168
3.111AspTyr: 3.111 ± 0.261
0.0AspXaa: 0.0 ± 0.0
Glu
5.666GluAla: 5.666 ± 0.426
0.634GluCys: 0.634 ± 0.132
4.206GluAsp: 4.206 ± 0.314
5.724GluGlu: 5.724 ± 0.456
2.977GluPhe: 2.977 ± 0.262
4.974GluGly: 4.974 ± 0.335
1.056GluHis: 1.056 ± 0.177
3.4GluIle: 3.4 ± 0.275
4.129GluLys: 4.129 ± 0.383
6.031GluLeu: 6.031 ± 0.389
1.498GluMet: 1.498 ± 0.201
2.843GluAsn: 2.843 ± 0.239
2.228GluPro: 2.228 ± 0.295
2.689GluGln: 2.689 ± 0.234
2.958GluArg: 2.958 ± 0.273
3.303GluSer: 3.303 ± 0.32
3.784GluThr: 3.784 ± 0.256
5.416GluVal: 5.416 ± 0.4
0.845GluTrp: 0.845 ± 0.132
2.881GluTyr: 2.881 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
2.535PheAla: 2.535 ± 0.215
0.384PheCys: 0.384 ± 0.105
3.131PheAsp: 3.131 ± 0.27
2.093PheGlu: 2.093 ± 0.219
1.479PhePhe: 1.479 ± 0.162
2.996PheGly: 2.996 ± 0.277
0.73PheHis: 0.73 ± 0.138
2.439PheIle: 2.439 ± 0.223
1.767PheLys: 1.767 ± 0.219
2.862PheLeu: 2.862 ± 0.21
1.037PheMet: 1.037 ± 0.152
1.959PheAsn: 1.959 ± 0.218
1.556PhePro: 1.556 ± 0.194
1.575PheGln: 1.575 ± 0.191
1.94PheArg: 1.94 ± 0.217
3.457PheSer: 3.457 ± 0.311
3.246PheThr: 3.246 ± 0.278
2.689PheVal: 2.689 ± 0.253
0.48PheTrp: 0.48 ± 0.11
1.959PheTyr: 1.959 ± 0.198
0.0PheXaa: 0.0 ± 0.0
Gly
6.223GlyAla: 6.223 ± 0.482
0.384GlyCys: 0.384 ± 0.086
5.493GlyAsp: 5.493 ± 0.471
5.263GlyGlu: 5.263 ± 0.379
3.131GlyPhe: 3.131 ± 0.232
7.625GlyGly: 7.625 ± 0.848
1.095GlyHis: 1.095 ± 0.132
3.649GlyIle: 3.649 ± 0.277
4.648GlyLys: 4.648 ± 0.507
5.224GlyLeu: 5.224 ± 0.319
1.594GlyMet: 1.594 ± 0.179
4.033GlyAsn: 4.033 ± 0.341
2.113GlyPro: 2.113 ± 0.24
2.843GlyGln: 2.843 ± 0.277
3.534GlyArg: 3.534 ± 0.257
6.184GlySer: 6.184 ± 0.547
6.05GlyThr: 6.05 ± 0.493
5.013GlyVal: 5.013 ± 0.35
1.037GlyTrp: 1.037 ± 0.177
3.092GlyTyr: 3.092 ± 0.24
0.0GlyXaa: 0.0 ± 0.0
His
0.787HisAla: 0.787 ± 0.136
0.192HisCys: 0.192 ± 0.064
1.095HisAsp: 1.095 ± 0.151
1.018HisGlu: 1.018 ± 0.135
0.672HisPhe: 0.672 ± 0.113
1.268HisGly: 1.268 ± 0.131
0.288HisHis: 0.288 ± 0.063
0.807HisIle: 0.807 ± 0.11
1.21HisLys: 1.21 ± 0.195
1.498HisLeu: 1.498 ± 0.175
0.365HisMet: 0.365 ± 0.085
0.73HisAsn: 0.73 ± 0.123
0.845HisPro: 0.845 ± 0.142
0.73HisGln: 0.73 ± 0.141
0.826HisArg: 0.826 ± 0.128
1.037HisSer: 1.037 ± 0.123
0.999HisThr: 0.999 ± 0.154
0.864HisVal: 0.864 ± 0.138
0.211HisTrp: 0.211 ± 0.066
0.672HisTyr: 0.672 ± 0.117
0.0HisXaa: 0.0 ± 0.0
Ile
4.072IleAla: 4.072 ± 0.302
0.423IleCys: 0.423 ± 0.091
4.187IleAsp: 4.187 ± 0.276
3.649IleGlu: 3.649 ± 0.252
1.671IlePhe: 1.671 ± 0.146
3.111IleGly: 3.111 ± 0.311
0.845IleHis: 0.845 ± 0.137
2.958IleIle: 2.958 ± 0.26
2.958IleLys: 2.958 ± 0.257
3.496IleLeu: 3.496 ± 0.289
1.056IleMet: 1.056 ± 0.15
3.246IleAsn: 3.246 ± 0.28
2.42IlePro: 2.42 ± 0.274
1.767IleGln: 1.767 ± 0.206
2.324IleArg: 2.324 ± 0.23
3.784IleSer: 3.784 ± 0.26
4.456IleThr: 4.456 ± 0.373
3.534IleVal: 3.534 ± 0.239
0.576IleTrp: 0.576 ± 0.113
1.844IleTyr: 1.844 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
4.782LysAla: 4.782 ± 0.432
0.634LysCys: 0.634 ± 0.116
3.572LysAsp: 3.572 ± 0.305
4.859LysGlu: 4.859 ± 0.475
2.17LysPhe: 2.17 ± 0.231
3.803LysGly: 3.803 ± 0.393
1.133LysHis: 1.133 ± 0.158
2.843LysIle: 2.843 ± 0.233
5.07LysLys: 5.07 ± 0.588
4.552LysLeu: 4.552 ± 0.422
1.882LysMet: 1.882 ± 0.22
2.497LysAsn: 2.497 ± 0.269
2.401LysPro: 2.401 ± 0.25
2.19LysGln: 2.19 ± 0.254
2.689LysArg: 2.689 ± 0.272
3.015LysSer: 3.015 ± 0.287
2.881LysThr: 2.881 ± 0.279
3.342LysVal: 3.342 ± 0.255
0.787LysTrp: 0.787 ± 0.125
2.343LysTyr: 2.343 ± 0.274
0.0LysXaa: 0.0 ± 0.0
Leu
5.455LeuAla: 5.455 ± 0.339
0.787LeuCys: 0.787 ± 0.139
5.263LeuAsp: 5.263 ± 0.336
5.512LeuGlu: 5.512 ± 0.403
2.708LeuPhe: 2.708 ± 0.256
4.725LeuGly: 4.725 ± 0.302
1.325LeuHis: 1.325 ± 0.178
3.419LeuIle: 3.419 ± 0.242
4.514LeuLys: 4.514 ± 0.37
5.09LeuLeu: 5.09 ± 0.392
2.286LeuMet: 2.286 ± 0.267
4.417LeuAsn: 4.417 ± 0.261
3.438LeuPro: 3.438 ± 0.227
2.939LeuGln: 2.939 ± 0.256
3.707LeuArg: 3.707 ± 0.271
5.263LeuSer: 5.263 ± 0.329
5.992LeuThr: 5.992 ± 0.384
4.667LeuVal: 4.667 ± 0.28
0.922LeuTrp: 0.922 ± 0.154
3.054LeuTyr: 3.054 ± 0.247
0.0LeuXaa: 0.0 ± 0.0
Met
1.921MetAla: 1.921 ± 0.213
0.154MetCys: 0.154 ± 0.05
1.133MetAsp: 1.133 ± 0.167
1.306MetGlu: 1.306 ± 0.153
0.96MetPhe: 0.96 ± 0.155
1.402MetGly: 1.402 ± 0.148
0.384MetHis: 0.384 ± 0.1
0.922MetIle: 0.922 ± 0.136
1.805MetLys: 1.805 ± 0.198
1.613MetLeu: 1.613 ± 0.215
0.615MetMet: 0.615 ± 0.101
1.248MetAsn: 1.248 ± 0.174
1.133MetPro: 1.133 ± 0.152
0.922MetGln: 0.922 ± 0.143
1.248MetArg: 1.248 ± 0.132
2.382MetSer: 2.382 ± 0.244
1.844MetThr: 1.844 ± 0.21
1.229MetVal: 1.229 ± 0.141
0.269MetTrp: 0.269 ± 0.071
0.576MetTyr: 0.576 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.649AsnAla: 3.649 ± 0.322
0.499AsnCys: 0.499 ± 0.105
3.15AsnAsp: 3.15 ± 0.287
2.382AsnGlu: 2.382 ± 0.176
2.574AsnPhe: 2.574 ± 0.212
4.129AsnGly: 4.129 ± 0.345
0.691AsnHis: 0.691 ± 0.119
3.284AsnIle: 3.284 ± 0.262
2.727AsnLys: 2.727 ± 0.282
3.918AsnLeu: 3.918 ± 0.261
1.076AsnMet: 1.076 ± 0.152
3.188AsnAsn: 3.188 ± 0.318
2.862AsnPro: 2.862 ± 0.252
2.055AsnGln: 2.055 ± 0.196
2.401AsnArg: 2.401 ± 0.22
3.265AsnSer: 3.265 ± 0.244
3.054AsnThr: 3.054 ± 0.364
3.303AsnVal: 3.303 ± 0.202
0.711AsnTrp: 0.711 ± 0.116
2.458AsnTyr: 2.458 ± 0.243
0.0AsnXaa: 0.0 ± 0.0
Pro
3.707ProAla: 3.707 ± 0.337
0.346ProCys: 0.346 ± 0.078
2.286ProAsp: 2.286 ± 0.248
3.284ProGlu: 3.284 ± 0.342
1.729ProPhe: 1.729 ± 0.206
3.611ProGly: 3.611 ± 0.304
0.499ProHis: 0.499 ± 0.094
1.882ProIle: 1.882 ± 0.191
2.42ProLys: 2.42 ± 0.296
2.439ProLeu: 2.439 ± 0.192
0.922ProMet: 0.922 ± 0.116
2.247ProAsn: 2.247 ± 0.231
1.46ProPro: 1.46 ± 0.2
1.172ProGln: 1.172 ± 0.163
1.44ProArg: 1.44 ± 0.169
2.958ProSer: 2.958 ± 0.301
3.688ProThr: 3.688 ± 0.286
3.111ProVal: 3.111 ± 0.193
0.442ProTrp: 0.442 ± 0.085
1.287ProTyr: 1.287 ± 0.173
0.0ProXaa: 0.0 ± 0.0
Gln
2.65GlnAla: 2.65 ± 0.212
0.25GlnCys: 0.25 ± 0.085
2.055GlnAsp: 2.055 ± 0.186
2.535GlnGlu: 2.535 ± 0.271
1.383GlnPhe: 1.383 ± 0.163
3.035GlnGly: 3.035 ± 0.306
0.807GlnHis: 0.807 ± 0.149
2.439GlnIle: 2.439 ± 0.235
1.901GlnLys: 1.901 ± 0.245
3.822GlnLeu: 3.822 ± 0.244
0.98GlnMet: 0.98 ± 0.145
1.767GlnAsn: 1.767 ± 0.182
1.287GlnPro: 1.287 ± 0.175
2.113GlnGln: 2.113 ± 0.242
2.093GlnArg: 2.093 ± 0.186
2.19GlnSer: 2.19 ± 0.227
2.478GlnThr: 2.478 ± 0.278
2.631GlnVal: 2.631 ± 0.233
0.653GlnTrp: 0.653 ± 0.123
1.863GlnTyr: 1.863 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
2.9ArgAla: 2.9 ± 0.293
0.499ArgCys: 0.499 ± 0.096
2.977ArgAsp: 2.977 ± 0.237
2.996ArgGlu: 2.996 ± 0.245
1.786ArgPhe: 1.786 ± 0.166
3.054ArgGly: 3.054 ± 0.244
0.845ArgHis: 0.845 ± 0.138
2.382ArgIle: 2.382 ± 0.212
3.131ArgLys: 3.131 ± 0.313
3.592ArgLeu: 3.592 ± 0.307
1.018ArgMet: 1.018 ± 0.16
2.132ArgAsn: 2.132 ± 0.271
1.69ArgPro: 1.69 ± 0.241
1.517ArgGln: 1.517 ± 0.175
2.362ArgArg: 2.362 ± 0.234
2.9ArgSer: 2.9 ± 0.242
2.228ArgThr: 2.228 ± 0.213
3.246ArgVal: 3.246 ± 0.281
0.691ArgTrp: 0.691 ± 0.121
2.401ArgTyr: 2.401 ± 0.174
0.0ArgXaa: 0.0 ± 0.0
Ser
5.512SerAla: 5.512 ± 0.369
0.48SerCys: 0.48 ± 0.099
4.245SerAsp: 4.245 ± 0.26
4.321SerGlu: 4.321 ± 0.365
3.054SerPhe: 3.054 ± 0.247
7.03SerGly: 7.03 ± 0.543
1.018SerHis: 1.018 ± 0.147
3.323SerIle: 3.323 ± 0.306
2.958SerLys: 2.958 ± 0.252
5.109SerLeu: 5.109 ± 0.311
1.613SerMet: 1.613 ± 0.15
3.611SerAsn: 3.611 ± 0.351
3.015SerPro: 3.015 ± 0.235
2.362SerGln: 2.362 ± 0.206
2.574SerArg: 2.574 ± 0.212
5.493SerSer: 5.493 ± 0.733
5.032SerThr: 5.032 ± 0.517
4.245SerVal: 4.245 ± 0.293
0.653SerTrp: 0.653 ± 0.115
2.689SerTyr: 2.689 ± 0.213
0.0SerXaa: 0.0 ± 0.0
Thr
5.685ThrAla: 5.685 ± 0.441
0.461ThrCys: 0.461 ± 0.094
4.533ThrAsp: 4.533 ± 0.316
4.341ThrGlu: 4.341 ± 0.299
3.342ThrPhe: 3.342 ± 0.336
5.647ThrGly: 5.647 ± 0.394
1.114ThrHis: 1.114 ± 0.133
3.784ThrIle: 3.784 ± 0.339
3.515ThrLys: 3.515 ± 0.237
5.743ThrLeu: 5.743 ± 0.442
1.229ThrMet: 1.229 ± 0.141
3.534ThrAsn: 3.534 ± 0.376
3.899ThrPro: 3.899 ± 0.28
2.785ThrGln: 2.785 ± 0.227
2.458ThrArg: 2.458 ± 0.296
4.686ThrSer: 4.686 ± 0.469
5.781ThrThr: 5.781 ± 0.556
5.128ThrVal: 5.128 ± 0.403
1.076ThrTrp: 1.076 ± 0.166
2.785ThrTyr: 2.785 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
5.435ValAla: 5.435 ± 0.353
0.807ValCys: 0.807 ± 0.127
4.514ValAsp: 4.514 ± 0.33
4.898ValGlu: 4.898 ± 0.307
2.458ValPhe: 2.458 ± 0.23
5.013ValGly: 5.013 ± 0.391
1.076ValHis: 1.076 ± 0.175
3.649ValIle: 3.649 ± 0.269
3.207ValLys: 3.207 ± 0.266
4.494ValLeu: 4.494 ± 0.312
1.613ValMet: 1.613 ± 0.2
3.515ValAsn: 3.515 ± 0.321
2.574ValPro: 2.574 ± 0.225
2.804ValGln: 2.804 ± 0.256
2.958ValArg: 2.958 ± 0.234
4.821ValSer: 4.821 ± 0.285
5.474ValThr: 5.474 ± 0.461
4.571ValVal: 4.571 ± 0.355
0.96ValTrp: 0.96 ± 0.133
2.554ValTyr: 2.554 ± 0.23
0.0ValXaa: 0.0 ± 0.0
Trp
1.018TrpAla: 1.018 ± 0.146
0.096TrpCys: 0.096 ± 0.04
1.21TrpAsp: 1.21 ± 0.182
0.845TrpGlu: 0.845 ± 0.144
0.327TrpPhe: 0.327 ± 0.067
0.807TrpGly: 0.807 ± 0.132
0.154TrpHis: 0.154 ± 0.061
0.557TrpIle: 0.557 ± 0.09
0.615TrpLys: 0.615 ± 0.113
0.98TrpLeu: 0.98 ± 0.14
0.269TrpMet: 0.269 ± 0.084
0.787TrpAsn: 0.787 ± 0.128
0.23TrpPro: 0.23 ± 0.068
0.538TrpGln: 0.538 ± 0.105
0.538TrpArg: 0.538 ± 0.1
0.96TrpSer: 0.96 ± 0.155
1.229TrpThr: 1.229 ± 0.172
1.018TrpVal: 1.018 ± 0.155
0.384TrpTrp: 0.384 ± 0.111
0.672TrpTyr: 0.672 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.747TyrAla: 2.747 ± 0.183
0.442TyrCys: 0.442 ± 0.099
3.073TyrAsp: 3.073 ± 0.224
2.228TyrGlu: 2.228 ± 0.23
1.729TyrPhe: 1.729 ± 0.192
2.785TyrGly: 2.785 ± 0.256
0.672TyrHis: 0.672 ± 0.102
2.439TyrIle: 2.439 ± 0.207
2.19TyrLys: 2.19 ± 0.251
2.958TyrLeu: 2.958 ± 0.256
0.826TyrMet: 0.826 ± 0.138
2.708TyrAsn: 2.708 ± 0.196
1.056TyrPro: 1.056 ± 0.143
2.017TyrGln: 2.017 ± 0.202
1.901TyrArg: 1.901 ± 0.198
2.631TyrSer: 2.631 ± 0.224
3.111TyrThr: 3.111 ± 0.252
2.766TyrVal: 2.766 ± 0.228
0.653TyrTrp: 0.653 ± 0.106
2.074TyrTyr: 2.074 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 246 proteins (52067 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski