Plautia stali symbiont
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5007 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U3TMA8|U3TMA8_9ENTR Regulator of RpoD Rsd/AlgQ OS=Plautia stali symbiont OX=891974 GN=E05_00890 PE=4 SV=1
MM1 pKa = 7.67 NIGLFYY7 pKa = 10.91 GSSTCYY13 pKa = 10.55 TEE15 pKa = 3.77 MTAEE19 pKa = 4.93 KK20 pKa = 9.94 IRR22 pKa = 11.84 DD23 pKa = 4.19 FIGEE27 pKa = 4.16 EE28 pKa = 4.25 LVTLHH33 pKa = 6.38 NLKK36 pKa = 10.75 DD37 pKa = 4.08 DD38 pKa = 4.84 DD39 pKa = 4.63 PSLMEE44 pKa = 4.91 QYY46 pKa = 11.23 DD47 pKa = 4.27 LLILGIPTWDD57 pKa = 3.34 FGEE60 pKa = 4.35 LQEE63 pKa = 4.88 DD64 pKa = 4.36 WEE66 pKa = 4.75 AVWQQLPALNLQGKK80 pKa = 8.8 IVALYY85 pKa = 10.92 GMGDD89 pKa = 3.29 QVEE92 pKa = 4.19 YY93 pKa = 11.26 SEE95 pKa = 4.71 WFLDD99 pKa = 3.7 ALGMLHH105 pKa = 7.11 EE106 pKa = 5.47 LLLPMGVQFVGYY118 pKa = 8.83 WPLDD122 pKa = 3.59 GYY124 pKa = 11.21 QFTSKK129 pKa = 10.7 KK130 pKa = 9.95 PLTADD135 pKa = 3.48 GQQFVGLALDD145 pKa = 4.36 DD146 pKa = 4.13 VNQFEE151 pKa = 4.43 LSEE154 pKa = 4.09 EE155 pKa = 4.47 RR156 pKa = 11.84 VEE158 pKa = 4.38 QWCEE162 pKa = 3.67 QILTEE167 pKa = 4.42 TAALLL172 pKa = 3.98
Molecular weight: 19.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.821
IPC_protein 3.783
Toseland 3.592
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.037
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.897
Patrickios 0.985
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|U3TSC9|U3TSC9_9ENTR Bacteriophage transcriptional regulator OS=Plautia stali symbiont OX=891974 GN=E05_05880 PE=4 SV=1
MM1 pKa = 7.86 RR2 pKa = 11.84 LTVRR6 pKa = 11.84 NQQRR10 pKa = 11.84 IARR13 pKa = 11.84 LQRR16 pKa = 11.84 IDD18 pKa = 3.57 GAGSGANQQRR28 pKa = 11.84 AAANKK33 pKa = 8.5 VQLRR37 pKa = 11.84 LARR40 pKa = 11.84 RR41 pKa = 11.84 VIILQAKK48 pKa = 8.6 RR49 pKa = 11.84 RR50 pKa = 11.84 MPISSSLVKK59 pKa = 10.73 SSS61 pKa = 3.02
Molecular weight: 6.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.42
IPC2_protein 10.891
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.281
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.018
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.113
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5007
0
5007
1118627
39
1843
223.4
24.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.551 ± 0.05
1.071 ± 0.015
5.239 ± 0.028
5.27 ± 0.036
3.685 ± 0.025
7.355 ± 0.036
2.38 ± 0.017
5.392 ± 0.029
3.798 ± 0.033
11.121 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.762 ± 0.018
3.487 ± 0.028
4.543 ± 0.025
5.101 ± 0.036
6.137 ± 0.032
5.928 ± 0.027
5.105 ± 0.024
6.956 ± 0.027
1.505 ± 0.016
2.612 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here