Frischella perrara
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2270 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7RYZ4|A0A0A7RYZ4_FRIPE Histidine kinase OS=Frischella perrara OX=1267021 GN=FPB0191_00684 PE=4 SV=1
MM1 pKa = 7.6 TKK3 pKa = 9.75 TDD5 pKa = 3.52 DD6 pKa = 3.7 QLNNEE11 pKa = 4.17 IGQLLFKK18 pKa = 10.74 SSPNGAKK25 pKa = 9.96 KK26 pKa = 10.59 VIAQLEE32 pKa = 4.4 FTPEE36 pKa = 3.62 MDD38 pKa = 3.14 ACTCIFDD45 pKa = 4.51 YY46 pKa = 11.35 YY47 pKa = 10.33 NQNDD51 pKa = 3.69 EE52 pKa = 4.79 LNWYY56 pKa = 6.7 TLNSDD61 pKa = 3.16 ITSPLIKK68 pKa = 10.07 AVRR71 pKa = 11.84 KK72 pKa = 9.36 LRR74 pKa = 11.84 QYY76 pKa = 11.31 YY77 pKa = 9.86 IDD79 pKa = 4.13 NNLTNGLPAWIGCIITVDD97 pKa = 3.4 IEE99 pKa = 3.88 NAKK102 pKa = 9.9 IDD104 pKa = 3.83 FEE106 pKa = 4.54 FKK108 pKa = 10.52 YY109 pKa = 10.86 EE110 pKa = 3.96 PFIDD114 pKa = 4.18 VYY116 pKa = 11.06 SEE118 pKa = 3.97 YY119 pKa = 11.08 DD120 pKa = 3.4 GDD122 pKa = 5.66 DD123 pKa = 3.67 DD124 pKa = 6.35 DD125 pKa = 4.82 EE126 pKa = 4.53
Molecular weight: 14.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.948
IPC_protein 3.923
Toseland 3.706
ProMoST 4.05
Dawson 3.91
Bjellqvist 4.113
Wikipedia 3.846
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.253
Thurlkill 3.757
EMBOSS 3.859
Sillero 4.037
Patrickios 1.926
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|A0A0A7S8A4|A0A0A7S8A4_FRIPE Glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase OS=Frischella perrara OX=1267021 GN=DKK76_08010 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSS46 pKa = 4.14
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2270
0
2270
759465
41
3195
334.6
37.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.007 ± 0.057
1.087 ± 0.018
5.433 ± 0.038
5.627 ± 0.052
4.279 ± 0.035
6.016 ± 0.052
2.168 ± 0.027
8.605 ± 0.055
6.388 ± 0.05
10.341 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.024
5.671 ± 0.052
3.676 ± 0.025
4.79 ± 0.045
4.046 ± 0.037
6.372 ± 0.041
5.338 ± 0.031
5.943 ± 0.042
1.134 ± 0.019
3.771 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here