Synechococcus sp. GFB01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6B784|A0A0J6B784_9SYNE Uncharacterized protein OS=Synechococcus sp. GFB01 OX=1662190 GN=SYNGFB01_03965 PE=4 SV=1
MM1 pKa = 7.83PFFEE5 pKa = 4.67VLWHH9 pKa = 6.57GEE11 pKa = 4.08AIGDD15 pKa = 3.94GGDD18 pKa = 3.43LAEE21 pKa = 5.05ALQAYY26 pKa = 7.2ATVKK30 pKa = 10.44PDD32 pKa = 4.22DD33 pKa = 5.04GDD35 pKa = 3.49WQAACSSPGAEE46 pKa = 3.81PCIRR50 pKa = 11.84RR51 pKa = 11.84YY52 pKa = 10.76ASFDD56 pKa = 3.46AFLDD60 pKa = 3.83NADD63 pKa = 4.01EE64 pKa = 4.93LEE66 pKa = 4.64TIPVTAAMIAEE77 pKa = 4.7ALPPQGG83 pKa = 4.0

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6BI89|A0A0J6BI89_9SYNE S-adenosylmethionine tRNA ribosyltransferase (Fragment) OS=Synechococcus sp. GFB01 OX=1662190 GN=SYNGFB01_11225 PE=4 SV=1
MM1 pKa = 7.8ASRR4 pKa = 11.84PTRR7 pKa = 11.84ATRR10 pKa = 11.84ATSGQPGSSPSASIRR25 pKa = 11.84AAIRR29 pKa = 11.84ALPIRR34 pKa = 11.84LAAVPSRR41 pKa = 11.84LMAPSVPGGTGLSVVISTQRR61 pKa = 11.84RR62 pKa = 11.84PQRR65 pKa = 11.84LPTSLPKK72 pKa = 10.4VSPSLAAKK80 pKa = 10.0LAAKK84 pKa = 7.25PTSSRR89 pKa = 11.84GVSAAGSRR97 pKa = 11.84GTARR101 pKa = 11.84ASRR104 pKa = 11.84APVAADD110 pKa = 3.31PQTFSGPRR118 pKa = 11.84RR119 pKa = 11.84PPRR122 pKa = 11.84PSARR126 pKa = 11.84PRR128 pKa = 11.84ACLRR132 pKa = 11.84RR133 pKa = 11.84KK134 pKa = 8.99PRR136 pKa = 11.84RR137 pKa = 11.84VTSDD141 pKa = 2.98PSARR145 pKa = 11.84YY146 pKa = 7.88STTASQARR154 pKa = 11.84GPRR157 pKa = 11.84QPQRR161 pKa = 11.84IVPRR165 pKa = 11.84APACTQPAVVSSGRR179 pKa = 11.84RR180 pKa = 11.84WARR183 pKa = 11.84LISSRR188 pKa = 11.84FTSQAA193 pKa = 2.87

Molecular weight: 20.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1749

0

1749

432581

29

1368

247.3

26.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.115 ± 0.084

1.202 ± 0.025

4.819 ± 0.046

5.685 ± 0.058

2.951 ± 0.042

8.815 ± 0.057

2.079 ± 0.031

3.955 ± 0.051

1.966 ± 0.05

13.077 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.838 ± 0.027

2.065 ± 0.036

6.175 ± 0.057

4.352 ± 0.042

8.099 ± 0.067

5.652 ± 0.042

4.425 ± 0.042

7.029 ± 0.056

1.728 ± 0.033

1.813 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski