Amino acid dipepetide frequency for Alteromonadaceae bacterium Bs31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.457AlaAla: 8.457 ± 0.109	1.165AlaCys: 1.165 ± 0.032	4.758AlaAsp: 4.758 ± 0.058	6.135AlaGlu: 6.135 ± 0.079	3.492AlaPhe: 3.492 ± 0.055	6.61AlaGly: 6.61 ± 0.097	1.731AlaHis: 1.731 ± 0.039	5.496AlaIle: 5.496 ± 0.069	4.55AlaLys: 4.55 ± 0.077	9.946AlaLeu: 9.946 ± 0.103	2.221AlaMet: 2.221 ± 0.048	3.615AlaAsn: 3.615 ± 0.061	3.16AlaPro: 3.16 ± 0.054	3.579AlaGln: 3.579 ± 0.059	4.094AlaArg: 4.094 ± 0.057	6.275AlaSer: 6.275 ± 0.083	4.038AlaThr: 4.038 ± 0.062	5.927AlaVal: 5.927 ± 0.079	1.096AlaTrp: 1.096 ± 0.03	2.612AlaTyr: 2.612 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.888CysAla: 0.888 ± 0.029	0.178CysCys: 0.178 ± 0.011	0.608CysAsp: 0.608 ± 0.022	0.705CysGlu: 0.705 ± 0.025	0.47CysPhe: 0.47 ± 0.018	0.989CysGly: 0.989 ± 0.028	0.323CysHis: 0.323 ± 0.02	0.59CysIle: 0.59 ± 0.023	0.502CysLys: 0.502 ± 0.023	1.068CysLeu: 1.068 ± 0.027	0.228CysMet: 0.228 ± 0.012	0.413CysAsn: 0.413 ± 0.017	0.462CysPro: 0.462 ± 0.019	0.361CysGln: 0.361 ± 0.016	0.485CysArg: 0.485 ± 0.019	0.887CysSer: 0.887 ± 0.024	0.491CysThr: 0.491 ± 0.019	0.743CysVal: 0.743 ± 0.027	0.166CysTrp: 0.166 ± 0.01	0.336CysTyr: 0.336 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.57AspAla: 4.57 ± 0.069	0.559AspCys: 0.559 ± 0.026	3.041AspAsp: 3.041 ± 0.067	3.865AspGlu: 3.865 ± 0.067	2.485AspPhe: 2.485 ± 0.04	4.199AspGly: 4.199 ± 0.099	1.029AspHis: 1.029 ± 0.029	3.864AspIle: 3.864 ± 0.061	3.051AspLys: 3.051 ± 0.046	5.125AspLeu: 5.125 ± 0.076	1.244AspMet: 1.244 ± 0.031	2.408AspAsn: 2.408 ± 0.046	2.221AspPro: 2.221 ± 0.044	1.909AspGln: 1.909 ± 0.044	2.379AspArg: 2.379 ± 0.046	3.811AspSer: 3.811 ± 0.084	2.927AspThr: 2.927 ± 0.071	3.631AspVal: 3.631 ± 0.059	0.902AspTrp: 0.902 ± 0.027	2.129AspTyr: 2.129 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.599GluAla: 5.599 ± 0.072	0.519GluCys: 0.519 ± 0.02	3.242GluAsp: 3.242 ± 0.055	4.145GluGlu: 4.145 ± 0.078	2.516GluPhe: 2.516 ± 0.047	4.124GluGly: 4.124 ± 0.056	1.612GluHis: 1.612 ± 0.037	3.831GluIle: 3.831 ± 0.048	4.0GluLys: 4.0 ± 0.067	7.261GluLeu: 7.261 ± 0.097	1.489GluMet: 1.489 ± 0.035	3.19GluAsn: 3.19 ± 0.048	2.391GluPro: 2.391 ± 0.05	3.792GluGln: 3.792 ± 0.071	3.376GluArg: 3.376 ± 0.063	3.962GluSer: 3.962 ± 0.065	3.069GluThr: 3.069 ± 0.051	4.048GluVal: 4.048 ± 0.063	0.798GluTrp: 0.798 ± 0.024	1.914GluTyr: 1.914 ± 0.041	0.001GluXaa: 0.001 ± 0.001
Phe	3.436PheAla: 3.436 ± 0.053	0.554PheCys: 0.554 ± 0.021	2.805PheAsp: 2.805 ± 0.044	2.533PheGlu: 2.533 ± 0.047	1.812PhePhe: 1.812 ± 0.044	3.071PheGly: 3.071 ± 0.057	0.8PheHis: 0.8 ± 0.024	2.514PheIle: 2.514 ± 0.052	1.938PheLys: 1.938 ± 0.041	3.525PheLeu: 3.525 ± 0.066	0.88PheMet: 0.88 ± 0.026	1.917PheAsn: 1.917 ± 0.04	1.536PhePro: 1.536 ± 0.034	1.229PheGln: 1.229 ± 0.03	1.727PheArg: 1.727 ± 0.038	3.625PheSer: 3.625 ± 0.054	2.353PheThr: 2.353 ± 0.047	2.808PheVal: 2.808 ± 0.049	0.552PheTrp: 0.552 ± 0.021	1.439PheTyr: 1.439 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	5.727GlyAla: 5.727 ± 0.089	0.855GlyCys: 0.855 ± 0.026	4.185GlyAsp: 4.185 ± 0.078	4.915GlyGlu: 4.915 ± 0.07	3.262GlyPhe: 3.262 ± 0.047	5.693GlyGly: 5.693 ± 0.129	1.482GlyHis: 1.482 ± 0.031	4.293GlyIle: 4.293 ± 0.059	3.874GlyLys: 3.874 ± 0.057	7.015GlyLeu: 7.015 ± 0.086	1.808GlyMet: 1.808 ± 0.04	2.971GlyAsn: 2.971 ± 0.08	1.93GlyPro: 1.93 ± 0.042	2.372GlyGln: 2.372 ± 0.042	3.307GlyArg: 3.307 ± 0.059	5.333GlySer: 5.333 ± 0.116	3.63GlyThr: 3.63 ± 0.092	5.127GlyVal: 5.127 ± 0.071	1.062GlyTrp: 1.062 ± 0.032	2.448GlyTyr: 2.448 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.633HisAla: 1.633 ± 0.033	0.331HisCys: 0.331 ± 0.016	0.975HisAsp: 0.975 ± 0.028	1.133HisGlu: 1.133 ± 0.031	1.077HisPhe: 1.077 ± 0.028	1.514HisGly: 1.514 ± 0.037	0.611HisHis: 0.611 ± 0.027	1.333HisIle: 1.333 ± 0.025	1.062HisLys: 1.062 ± 0.03	2.101HisLeu: 2.101 ± 0.038	0.504HisMet: 0.504 ± 0.02	0.912HisAsn: 0.912 ± 0.025	1.07HisPro: 1.07 ± 0.026	0.922HisGln: 0.922 ± 0.028	1.083HisArg: 1.083 ± 0.027	1.575HisSer: 1.575 ± 0.031	1.094HisThr: 1.094 ± 0.029	1.152HisVal: 1.152 ± 0.028	0.37HisTrp: 0.37 ± 0.018	0.906HisTyr: 0.906 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.712IleAla: 5.712 ± 0.077	0.655IleCys: 0.655 ± 0.021	3.989IleAsp: 3.989 ± 0.059	4.311IleGlu: 4.311 ± 0.058	2.224IlePhe: 2.224 ± 0.048	4.035IleGly: 4.035 ± 0.059	1.289IleHis: 1.289 ± 0.034	3.114IleIle: 3.114 ± 0.054	2.981IleLys: 2.981 ± 0.052	4.827IleLeu: 4.827 ± 0.074	1.053IleMet: 1.053 ± 0.031	2.872IleAsn: 2.872 ± 0.057	2.516IlePro: 2.516 ± 0.041	2.005IleGln: 2.005 ± 0.036	2.848IleArg: 2.848 ± 0.049	4.619IleSer: 4.619 ± 0.062	3.353IleThr: 3.353 ± 0.077	3.995IleVal: 3.995 ± 0.058	0.645IleTrp: 0.645 ± 0.021	1.799IleTyr: 1.799 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.697LysAla: 4.697 ± 0.086	0.367LysCys: 0.367 ± 0.018	2.579LysAsp: 2.579 ± 0.054	3.051LysGlu: 3.051 ± 0.063	1.532LysPhe: 1.532 ± 0.032	3.299LysGly: 3.299 ± 0.051	1.282LysHis: 1.282 ± 0.032	3.184LysIle: 3.184 ± 0.057	3.268LysLys: 3.268 ± 0.07	5.214LysLeu: 5.214 ± 0.079	1.185LysMet: 1.185 ± 0.029	2.621LysAsn: 2.621 ± 0.053	2.457LysPro: 2.457 ± 0.046	2.48LysGln: 2.48 ± 0.05	2.684LysArg: 2.684 ± 0.053	3.159LysSer: 3.159 ± 0.051	2.788LysThr: 2.788 ± 0.053	3.303LysVal: 3.303 ± 0.065	0.537LysTrp: 0.537 ± 0.021	1.429LysTyr: 1.429 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	10.053LeuAla: 10.053 ± 0.107	1.175LeuCys: 1.175 ± 0.032	5.761LeuAsp: 5.761 ± 0.081	6.522LeuGlu: 6.522 ± 0.088	4.066LeuPhe: 4.066 ± 0.075	6.862LeuGly: 6.862 ± 0.074	2.061LeuHis: 2.061 ± 0.045	5.628LeuIle: 5.628 ± 0.078	5.22LeuLys: 5.22 ± 0.08	10.81LeuLeu: 10.81 ± 0.157	2.255LeuMet: 2.255 ± 0.044	4.351LeuAsn: 4.351 ± 0.062	4.566LeuPro: 4.566 ± 0.064	4.17LeuGln: 4.17 ± 0.058	4.938LeuArg: 4.938 ± 0.065	8.354LeuSer: 8.354 ± 0.086	4.64LeuThr: 4.64 ± 0.057	6.974LeuVal: 6.974 ± 0.097	1.173LeuTrp: 1.173 ± 0.033	2.768LeuTyr: 2.768 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.215MetAla: 2.215 ± 0.04	0.186MetCys: 0.186 ± 0.011	1.189MetAsp: 1.189 ± 0.027	1.401MetGlu: 1.401 ± 0.035	0.77MetPhe: 0.77 ± 0.023	1.581MetGly: 1.581 ± 0.034	0.475MetHis: 0.475 ± 0.018	1.127MetIle: 1.127 ± 0.029	1.242MetLys: 1.242 ± 0.032	2.41MetLeu: 2.41 ± 0.044	0.536MetMet: 0.536 ± 0.021	0.99MetAsn: 0.99 ± 0.027	1.116MetPro: 1.116 ± 0.031	0.947MetGln: 0.947 ± 0.028	1.188MetArg: 1.188 ± 0.03	1.735MetSer: 1.735 ± 0.037	1.08MetThr: 1.08 ± 0.031	1.47MetVal: 1.47 ± 0.033	0.216MetTrp: 0.216 ± 0.014	0.505MetTyr: 0.505 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.631AsnAla: 3.631 ± 0.066	0.506AsnCys: 0.506 ± 0.018	2.288AsnAsp: 2.288 ± 0.054	2.445AsnGlu: 2.445 ± 0.04	1.74AsnPhe: 1.74 ± 0.042	3.213AsnGly: 3.213 ± 0.082	0.813AsnHis: 0.813 ± 0.026	2.962AsnIle: 2.962 ± 0.055	2.298AsnLys: 2.298 ± 0.04	3.923AsnLeu: 3.923 ± 0.061	0.947AsnMet: 0.947 ± 0.024	2.205AsnAsn: 2.205 ± 0.049	2.164AsnPro: 2.164 ± 0.042	1.566AsnGln: 1.566 ± 0.034	2.037AsnArg: 2.037 ± 0.041	3.217AsnSer: 3.217 ± 0.065	2.703AsnThr: 2.703 ± 0.041	2.513AsnVal: 2.513 ± 0.054	0.753AsnTrp: 0.753 ± 0.029	1.528AsnTyr: 1.528 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	3.644ProAla: 3.644 ± 0.063	0.352ProCys: 0.352 ± 0.015	2.438ProAsp: 2.438 ± 0.044	3.433ProGlu: 3.433 ± 0.062	1.662ProPhe: 1.662 ± 0.032	2.962ProGly: 2.962 ± 0.051	0.789ProHis: 0.789 ± 0.023	2.115ProIle: 2.115 ± 0.037	1.82ProLys: 1.82 ± 0.035	4.118ProLeu: 4.118 ± 0.055	0.873ProMet: 0.873 ± 0.025	1.592ProAsn: 1.592 ± 0.04	1.503ProPro: 1.503 ± 0.042	1.641ProGln: 1.641 ± 0.036	1.587ProArg: 1.587 ± 0.038	2.827ProSer: 2.827 ± 0.047	2.021ProThr: 2.021 ± 0.073	3.01ProVal: 3.01 ± 0.05	0.565ProTrp: 0.565 ± 0.023	1.262ProTyr: 1.262 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.284GlnAla: 4.284 ± 0.069	0.405GlnCys: 0.405 ± 0.018	1.737GlnAsp: 1.737 ± 0.036	2.339GlnGlu: 2.339 ± 0.042	1.57GlnPhe: 1.57 ± 0.034	2.651GlnGly: 2.651 ± 0.042	1.088GlnHis: 1.088 ± 0.03	2.141GlnIle: 2.141 ± 0.039	2.114GlnLys: 2.114 ± 0.043	4.649GlnLeu: 4.649 ± 0.072	0.965GlnMet: 0.965 ± 0.026	1.632GlnAsn: 1.632 ± 0.033	1.486GlnPro: 1.486 ± 0.033	2.776GlnGln: 2.776 ± 0.059	2.244GlnArg: 2.244 ± 0.043	2.642GlnSer: 2.642 ± 0.048	1.824GlnThr: 1.824 ± 0.035	2.66GlnVal: 2.66 ± 0.048	0.68GlnTrp: 0.68 ± 0.019	1.161GlnTyr: 1.161 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	3.924ArgAla: 3.924 ± 0.058	0.478ArgCys: 0.478 ± 0.02	2.557ArgAsp: 2.557 ± 0.045	3.368ArgGlu: 3.368 ± 0.057	2.268ArgPhe: 2.268 ± 0.038	2.925ArgGly: 2.925 ± 0.048	1.069ArgHis: 1.069 ± 0.03	2.987ArgIle: 2.987 ± 0.05	2.532ArgLys: 2.532 ± 0.054	5.071ArgLeu: 5.071 ± 0.07	1.136ArgMet: 1.136 ± 0.026	1.945ArgAsn: 1.945 ± 0.039	1.715ArgPro: 1.715 ± 0.042	1.93ArgGln: 1.93 ± 0.041	2.438ArgArg: 2.438 ± 0.048	3.157ArgSer: 3.157 ± 0.05	2.077ArgThr: 2.077 ± 0.045	3.364ArgVal: 3.364 ± 0.053	0.799ArgTrp: 0.799 ± 0.027	1.941ArgTyr: 1.941 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.509SerAla: 6.509 ± 0.076	0.799SerCys: 0.799 ± 0.03	4.04SerAsp: 4.04 ± 0.053	4.658SerGlu: 4.658 ± 0.059	2.998SerPhe: 2.998 ± 0.049	6.066SerGly: 6.066 ± 0.114	1.5SerHis: 1.5 ± 0.032	4.276SerIle: 4.276 ± 0.071	3.349SerLys: 3.349 ± 0.048	7.371SerLeu: 7.371 ± 0.086	1.593SerMet: 1.593 ± 0.037	3.069SerAsn: 3.069 ± 0.056	2.898SerPro: 2.898 ± 0.047	2.6SerGln: 2.6 ± 0.047	3.214SerArg: 3.214 ± 0.053	9.348SerSer: 9.348 ± 0.507	3.88SerThr: 3.88 ± 0.075	4.893SerVal: 4.893 ± 0.07	1.105SerTrp: 1.105 ± 0.031	2.46SerTyr: 2.46 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.585ThrAla: 4.585 ± 0.071	0.448ThrCys: 0.448 ± 0.02	2.68ThrAsp: 2.68 ± 0.066	3.147ThrGlu: 3.147 ± 0.055	2.025ThrPhe: 2.025 ± 0.047	4.028ThrGly: 4.028 ± 0.086	1.007ThrHis: 1.007 ± 0.025	3.12ThrIle: 3.12 ± 0.075	1.951ThrLys: 1.951 ± 0.037	5.829ThrLeu: 5.829 ± 0.079	1.007ThrMet: 1.007 ± 0.023	1.907ThrAsn: 1.907 ± 0.043	2.573ThrPro: 2.573 ± 0.062	1.956ThrGln: 1.956 ± 0.038	2.293ThrArg: 2.293 ± 0.041	3.466ThrSer: 3.466 ± 0.064	2.609ThrThr: 2.609 ± 0.057	3.61ThrVal: 3.61 ± 0.069	0.599ThrTrp: 0.599 ± 0.026	1.448ThrTyr: 1.448 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	6.022ValAla: 6.022 ± 0.068	0.771ValCys: 0.771 ± 0.025	4.154ValAsp: 4.154 ± 0.066	4.397ValGlu: 4.397 ± 0.054	3.003ValPhe: 3.003 ± 0.051	4.314ValGly: 4.314 ± 0.072	1.322ValHis: 1.322 ± 0.029	3.883ValIle: 3.883 ± 0.055	3.321ValLys: 3.321 ± 0.057	6.979ValLeu: 6.979 ± 0.086	1.498ValMet: 1.498 ± 0.033	3.029ValAsn: 3.029 ± 0.053	2.626ValPro: 2.626 ± 0.049	2.221ValGln: 2.221 ± 0.041	3.038ValArg: 3.038 ± 0.048	5.201ValSer: 5.201 ± 0.073	3.364ValThr: 3.364 ± 0.068	5.061ValVal: 5.061 ± 0.071	0.77ValTrp: 0.77 ± 0.024	2.021ValTyr: 2.021 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.954TrpAla: 0.954 ± 0.028	0.152TrpCys: 0.152 ± 0.01	0.677TrpAsp: 0.677 ± 0.023	0.662TrpGlu: 0.662 ± 0.024	0.59TrpPhe: 0.59 ± 0.024	0.952TrpGly: 0.952 ± 0.029	0.356TrpHis: 0.356 ± 0.015	0.657TrpIle: 0.657 ± 0.024	0.531TrpLys: 0.531 ± 0.019	1.752TrpLeu: 1.752 ± 0.043	0.334TrpMet: 0.334 ± 0.015	0.591TrpAsn: 0.591 ± 0.026	0.543TrpPro: 0.543 ± 0.022	0.946TrpGln: 0.946 ± 0.025	0.815TrpArg: 0.815 ± 0.025	0.91TrpSer: 0.91 ± 0.027	0.57TrpThr: 0.57 ± 0.023	0.875TrpVal: 0.875 ± 0.027	0.218TrpTrp: 0.218 ± 0.014	0.453TrpTyr: 0.453 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.419TyrAla: 2.419 ± 0.04	0.436TyrCys: 0.436 ± 0.021	1.648TyrAsp: 1.648 ± 0.048	1.714TyrGlu: 1.714 ± 0.035	1.493TyrPhe: 1.493 ± 0.034	2.201TyrGly: 2.201 ± 0.039	0.72TyrHis: 0.72 ± 0.026	1.726TyrIle: 1.726 ± 0.037	1.513TyrLys: 1.513 ± 0.029	3.352TyrLeu: 3.352 ± 0.055	0.618TyrMet: 0.618 ± 0.02	1.221TyrAsn: 1.221 ± 0.034	1.28TyrPro: 1.28 ± 0.031	1.644TyrGln: 1.644 ± 0.035	1.903TyrArg: 1.903 ± 0.036	2.524TyrSer: 2.524 ± 0.043	1.774TyrThr: 1.774 ± 0.041	1.833TyrVal: 1.833 ± 0.034	0.535TyrTrp: 0.535 ± 0.021	1.141TyrTyr: 1.141 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.01

Statistics based on 4297 proteins (1444979 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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