Frederiksenia canicola
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1971 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4WDU5|A0A3N4WDU5_9PAST Uncharacterized protein OS=Frederiksenia canicola OX=123824 GN=EDC49_1336 PE=4 SV=1
MM1 pKa = 7.81 SDD3 pKa = 2.79 IQVPLTFTDD12 pKa = 3.72 AAAKK16 pKa = 9.6 KK17 pKa = 9.6 VKK19 pKa = 10.5 SLIEE23 pKa = 4.49 GEE25 pKa = 4.46 DD26 pKa = 3.6 NPNLRR31 pKa = 11.84 LRR33 pKa = 11.84 VYY35 pKa = 9.12 ITGGGCSGFQYY46 pKa = 11.03 GFTFDD51 pKa = 4.83 DD52 pKa = 3.68 QVNDD56 pKa = 3.5 GDD58 pKa = 4.74 LTIEE62 pKa = 4.06 NQNVGLVVDD71 pKa = 4.64 PMSLQYY77 pKa = 11.06 LIGGTVDD84 pKa = 3.37 YY85 pKa = 11.67 VEE87 pKa = 4.5 GLEE90 pKa = 4.06 GSRR93 pKa = 11.84 FVVSNPNASSTCGCGSSFSII113 pKa = 5.02
Molecular weight: 12.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.732
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.062
DTASelect 4.279
Thurlkill 3.795
EMBOSS 3.884
Sillero 4.062
Patrickios 1.939
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|A0A3N4WTB1|A0A3N4WTB1_9PAST Uncharacterized protein (TIGR00156 family) OS=Frederiksenia canicola OX=123824 GN=EDC49_1315 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1971
0
1971
636187
29
3668
322.8
36.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.688 ± 0.064
0.99 ± 0.021
5.014 ± 0.042
6.347 ± 0.058
4.468 ± 0.051
6.71 ± 0.048
2.11 ± 0.027
6.704 ± 0.042
6.117 ± 0.048
10.516 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.382 ± 0.032
4.637 ± 0.06
3.874 ± 0.038
4.888 ± 0.054
4.451 ± 0.043
5.809 ± 0.039
5.181 ± 0.043
6.819 ± 0.045
1.18 ± 0.02
3.113 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here