Apis mellifera associated microvirus 7
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5A1|A0A3Q8U5A1_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 7 OX=2494794 PE=4 SV=1
MM1 pKa = 6.79 YY2 pKa = 10.31 ACAVFDD8 pKa = 4.22 KK9 pKa = 10.86 KK10 pKa = 11.12 VNAYY14 pKa = 7.72 MYY16 pKa = 9.58 PQFFRR21 pKa = 11.84 TKK23 pKa = 10.56 GEE25 pKa = 3.94 ATRR28 pKa = 11.84 SFMDD32 pKa = 3.34 AVGAEE37 pKa = 3.71 AAPFRR42 pKa = 11.84 KK43 pKa = 9.42 HH44 pKa = 6.84 AEE46 pKa = 4.17 DD47 pKa = 4.54 YY48 pKa = 9.74 VFCYY52 pKa = 10.27 LGQYY56 pKa = 10.49 DD57 pKa = 4.9 DD58 pKa = 3.81 NTGQFEE64 pKa = 4.33 NAPGNLPEE72 pKa = 4.84 ILMTAMDD79 pKa = 5.11 CLTIDD84 pKa = 3.76 GAVNN88 pKa = 3.44
Molecular weight: 9.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.3
IPC2_protein 4.584
IPC_protein 4.418
Toseland 4.24
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.304
Rodwell 4.253
Grimsley 4.151
Solomon 4.38
Lehninger 4.329
Nozaki 4.507
DTASelect 4.698
Thurlkill 4.279
EMBOSS 4.317
Sillero 4.533
Patrickios 0.947
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.454
Protein with the highest isoelectric point:
>tr|A0A3S8UTL2|A0A3S8UTL2_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 7 OX=2494794 PE=4 SV=1
MM1 pKa = 7.37 SCYY4 pKa = 10.61 GPIAAYY10 pKa = 9.87 RR11 pKa = 11.84 PPRR14 pKa = 11.84 KK15 pKa = 9.81 EE16 pKa = 4.12 GGDD19 pKa = 3.28 KK20 pKa = 10.73 RR21 pKa = 11.84 LVFDD25 pKa = 4.19 PRR27 pKa = 11.84 KK28 pKa = 9.83 SEE30 pKa = 3.72 TGVRR34 pKa = 11.84 MLIPCGRR41 pKa = 11.84 CTGCRR46 pKa = 11.84 LEE48 pKa = 5.96 RR49 pKa = 11.84 SRR51 pKa = 11.84 QWAMRR56 pKa = 11.84 CMHH59 pKa = 7.32 EE60 pKa = 4.29 KK61 pKa = 10.47 QMHH64 pKa = 5.76 NASSFLTLTYY74 pKa = 11.13 DD75 pKa = 4.63 DD76 pKa = 4.31 VNLPEE81 pKa = 5.1 GGSLCMDD88 pKa = 4.49 HH89 pKa = 6.14 YY90 pKa = 11.19 QKK92 pKa = 10.88 FMKK95 pKa = 10.34 RR96 pKa = 11.84 LRR98 pKa = 11.84 HH99 pKa = 5.68 RR100 pKa = 11.84 FGDD103 pKa = 4.4 GIRR106 pKa = 11.84 FCGCGEE112 pKa = 4.12 YY113 pKa = 11.03 GDD115 pKa = 4.1 VTSRR119 pKa = 11.84 PHH121 pKa = 5.02 YY122 pKa = 10.47 HH123 pKa = 6.95 ILLLNVDD130 pKa = 4.22 FGDD133 pKa = 3.34 RR134 pKa = 11.84 RR135 pKa = 11.84 FYY137 pKa = 10.9 KK138 pKa = 10.41 SSEE141 pKa = 3.95 GSGLPLYY148 pKa = 10.34 VSKK151 pKa = 10.34 EE152 pKa = 3.99 LSSLWPYY159 pKa = 10.87 GFSNLGDD166 pKa = 3.4 VDD168 pKa = 4.85 FDD170 pKa = 3.62 SCAYY174 pKa = 8.0 VARR177 pKa = 11.84 YY178 pKa = 9.09 VMKK181 pKa = 10.5 KK182 pKa = 10.6 VIGNDD187 pKa = 3.26 TFDD190 pKa = 3.26 SSLYY194 pKa = 10.14 DD195 pKa = 3.34 WEE197 pKa = 4.31 TGIVKK202 pKa = 8.61 EE203 pKa = 4.26 APFMTMSRR211 pKa = 11.84 RR212 pKa = 11.84 PGLGSSWYY220 pKa = 10.24 KK221 pKa = 10.76 KK222 pKa = 10.35 FGAHH226 pKa = 6.02 AYY228 pKa = 8.65 EE229 pKa = 4.09 WDD231 pKa = 3.61 SVVFKK236 pKa = 10.7 GVEE239 pKa = 3.91 VPPPRR244 pKa = 11.84 FYY246 pKa = 11.32 DD247 pKa = 3.49 SLRR250 pKa = 11.84 IGPAINDD257 pKa = 3.39 NLKK260 pKa = 10.14 KK261 pKa = 10.05 KK262 pKa = 9.88 RR263 pKa = 11.84 RR264 pKa = 11.84 RR265 pKa = 11.84 KK266 pKa = 9.5 AALKK270 pKa = 10.52 RR271 pKa = 11.84 ADD273 pKa = 3.44 NTLARR278 pKa = 11.84 LRR280 pKa = 11.84 VKK282 pKa = 10.43 EE283 pKa = 4.19 VVALARR289 pKa = 11.84 LNLKK293 pKa = 9.87 GRR295 pKa = 11.84 SLL297 pKa = 3.67
Molecular weight: 34.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.916
IPC2_protein 9.019
IPC_protein 8.975
Toseland 9.531
ProMoST 9.385
Dawson 9.838
Bjellqvist 9.619
Wikipedia 10.028
Rodwell 10.058
Grimsley 9.926
Solomon 9.867
Lehninger 9.823
Nozaki 9.721
DTASelect 9.56
Thurlkill 9.677
EMBOSS 9.984
Sillero 9.809
Patrickios 4.952
IPC_peptide 9.867
IPC2_peptide 8.521
IPC2.peptide.svr19 7.896
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1425
88
533
237.5
26.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.895 ± 1.926
1.684 ± 0.668
5.474 ± 0.442
4.491 ± 0.895
4.842 ± 0.504
7.579 ± 0.421
1.965 ± 0.265
3.368 ± 0.44
3.789 ± 0.893
8.281 ± 0.5
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.368 ± 0.447
4.842 ± 0.554
5.544 ± 1.011
3.719 ± 0.724
6.947 ± 0.956
7.93 ± 0.818
4.632 ± 0.697
7.088 ± 0.533
0.982 ± 0.367
3.579 ± 0.788
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here