Sporolactobacillus sp. THM7-4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillus; unclassified Sporolactobacillus

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q4IRK1|A0A4Q4IRK1_9BACL Uncharacterized protein OS=Sporolactobacillus sp. THM7-4 OX=2511170 GN=EWI07_00595 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 10.48YY3 pKa = 10.71VIVDD7 pKa = 3.6KK8 pKa = 8.15EE9 pKa = 4.33TCIACGACGCAAPDD23 pKa = 3.41VFTYY27 pKa = 11.02DD28 pKa = 3.43DD29 pKa = 4.97DD30 pKa = 4.0GTSRR34 pKa = 11.84VTLDD38 pKa = 3.69DD39 pKa = 3.56NQGVVDD45 pKa = 5.1IPDD48 pKa = 4.4DD49 pKa = 4.21LLDD52 pKa = 4.1DD53 pKa = 4.88VIDD56 pKa = 4.32AFEE59 pKa = 4.47GCPTEE64 pKa = 4.36SIKK67 pKa = 10.79MSDD70 pKa = 3.41SPFNGNPLKK79 pKa = 11.02GEE81 pKa = 4.27SEE83 pKa = 4.28ASS85 pKa = 3.2

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1W7J4|A0A4V1W7J4_9BACL Carbon-nitrogen family hydrolase OS=Sporolactobacillus sp. THM7-4 OX=2511170 GN=EWI07_08915 PE=3 SV=1
MM1 pKa = 7.26SRR3 pKa = 11.84LVRR6 pKa = 11.84QLSRR10 pKa = 11.84LYY12 pKa = 9.68RR13 pKa = 11.84QFSRR17 pKa = 11.84FYY19 pKa = 8.98RR20 pKa = 11.84QLSRR24 pKa = 11.84LYY26 pKa = 9.23RR27 pKa = 11.84QLSRR31 pKa = 11.84LVRR34 pKa = 11.84QFSRR38 pKa = 11.84FVRR41 pKa = 11.84QLSRR45 pKa = 11.84SVRR48 pKa = 11.84QLSFFVRR55 pKa = 11.84QFNRR59 pKa = 11.84FVRR62 pKa = 11.84QFSRR66 pKa = 11.84FVRR69 pKa = 11.84QFNRR73 pKa = 11.84SVRR76 pKa = 11.84QFNRR80 pKa = 11.84FVRR83 pKa = 11.84QFSHH87 pKa = 7.13QIRR90 pKa = 11.84QLSRR94 pKa = 11.84SVRR97 pKa = 11.84QFKK100 pKa = 10.62RR101 pKa = 11.84SVRR104 pKa = 11.84QFNRR108 pKa = 11.84LVRR111 pKa = 11.84QLSRR115 pKa = 11.84YY116 pKa = 8.81

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2760

0

2760

852718

26

1515

309.0

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.404 ± 0.046

0.864 ± 0.014

5.217 ± 0.039

6.24 ± 0.049

4.427 ± 0.033

7.219 ± 0.045

2.253 ± 0.021

7.364 ± 0.049

6.439 ± 0.042

9.833 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.021

3.981 ± 0.031

3.961 ± 0.025

3.747 ± 0.031

5.354 ± 0.043

6.374 ± 0.048

5.234 ± 0.029

6.94 ± 0.04

1.006 ± 0.017

3.436 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski