Amino acid dipepetide frequency for Sporolactobacillus sp. THM7-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.745AlaAla: 6.745 ± 0.115
0.793AlaCys: 0.793 ± 0.029
4.054AlaAsp: 4.054 ± 0.075
4.772AlaGlu: 4.772 ± 0.096
3.346AlaPhe: 3.346 ± 0.068
6.376AlaGly: 6.376 ± 0.09
1.419AlaHis: 1.419 ± 0.04
5.669AlaIle: 5.669 ± 0.086
4.082AlaLys: 4.082 ± 0.082
7.541AlaLeu: 7.541 ± 0.096
2.072AlaMet: 2.072 ± 0.052
2.381AlaAsn: 2.381 ± 0.061
2.296AlaPro: 2.296 ± 0.061
2.295AlaGln: 2.295 ± 0.056
3.805AlaArg: 3.805 ± 0.073
4.393AlaSer: 4.393 ± 0.076
3.21AlaThr: 3.21 ± 0.078
5.898AlaVal: 5.898 ± 0.092
0.626AlaTrp: 0.626 ± 0.032
2.266AlaTyr: 2.266 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.026
0.115CysCys: 0.115 ± 0.01
0.473CysAsp: 0.473 ± 0.025
0.477CysGlu: 0.477 ± 0.022
0.462CysPhe: 0.462 ± 0.03
0.813CysGly: 0.813 ± 0.036
0.252CysHis: 0.252 ± 0.018
0.493CysIle: 0.493 ± 0.025
0.311CysLys: 0.311 ± 0.02
0.908CysLeu: 0.908 ± 0.034
0.216CysMet: 0.216 ± 0.014
0.253CysAsn: 0.253 ± 0.016
0.469CysPro: 0.469 ± 0.026
0.25CysGln: 0.25 ± 0.016
0.548CysArg: 0.548 ± 0.031
0.667CysSer: 0.667 ± 0.037
0.482CysThr: 0.482 ± 0.026
0.498CysVal: 0.498 ± 0.026
0.08CysTrp: 0.08 ± 0.008
0.291CysTyr: 0.291 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.313AspAla: 3.313 ± 0.062
0.428AspCys: 0.428 ± 0.022
2.544AspAsp: 2.544 ± 0.057
3.805AspGlu: 3.805 ± 0.078
2.306AspPhe: 2.306 ± 0.051
3.524AspGly: 3.524 ± 0.067
1.474AspHis: 1.474 ± 0.046
3.703AspIle: 3.703 ± 0.068
3.108AspLys: 3.108 ± 0.07
5.139AspLeu: 5.139 ± 0.085
1.282AspMet: 1.282 ± 0.035
1.645AspAsn: 1.645 ± 0.047
2.537AspPro: 2.537 ± 0.056
2.534AspGln: 2.534 ± 0.055
3.236AspArg: 3.236 ± 0.071
2.681AspSer: 2.681 ± 0.057
2.362AspThr: 2.362 ± 0.056
3.714AspVal: 3.714 ± 0.063
0.66AspTrp: 0.66 ± 0.029
2.18AspTyr: 2.18 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
5.054GluAla: 5.054 ± 0.089
0.425GluCys: 0.425 ± 0.022
2.996GluAsp: 2.996 ± 0.07
4.699GluGlu: 4.699 ± 0.104
1.862GluPhe: 1.862 ± 0.041
3.723GluGly: 3.723 ± 0.072
1.397GluHis: 1.397 ± 0.041
4.361GluIle: 4.361 ± 0.082
6.022GluLys: 6.022 ± 0.1
5.608GluLeu: 5.608 ± 0.09
1.905GluMet: 1.905 ± 0.055
3.082GluAsn: 3.082 ± 0.058
1.973GluPro: 1.973 ± 0.053
2.743GluGln: 2.743 ± 0.074
3.55GluArg: 3.55 ± 0.06
3.358GluSer: 3.358 ± 0.063
3.621GluThr: 3.621 ± 0.078
3.706GluVal: 3.706 ± 0.072
0.718GluTrp: 0.718 ± 0.028
1.594GluTyr: 1.594 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.029PheAla: 3.029 ± 0.07
0.43PheCys: 0.43 ± 0.02
2.386PheAsp: 2.386 ± 0.052
2.363PheGlu: 2.363 ± 0.056
2.226PhePhe: 2.226 ± 0.06
3.367PheGly: 3.367 ± 0.069
1.067PheHis: 1.067 ± 0.038
3.349PheIle: 3.349 ± 0.082
2.164PheLys: 2.164 ± 0.049
4.394PheLeu: 4.394 ± 0.088
1.036PheMet: 1.036 ± 0.034
1.875PheAsn: 1.875 ± 0.053
1.696PhePro: 1.696 ± 0.044
1.519PheGln: 1.519 ± 0.041
1.941PheArg: 1.941 ± 0.046
3.577PheSer: 3.577 ± 0.068
2.314PheThr: 2.314 ± 0.054
3.028PheVal: 3.028 ± 0.069
0.455PheTrp: 0.455 ± 0.028
2.052PheTyr: 2.052 ± 0.096
0.0PheXaa: 0.0 ± 0.0
Gly
5.25GlyAla: 5.25 ± 0.098
0.727GlyCys: 0.727 ± 0.032
3.156GlyAsp: 3.156 ± 0.07
4.066GlyGlu: 4.066 ± 0.077
3.464GlyPhe: 3.464 ± 0.06
5.312GlyGly: 5.312 ± 0.11
1.618GlyHis: 1.618 ± 0.041
5.844GlyIle: 5.844 ± 0.085
5.244GlyLys: 5.244 ± 0.084
7.098GlyLeu: 7.098 ± 0.112
2.228GlyMet: 2.228 ± 0.053
2.585GlyAsn: 2.585 ± 0.061
1.976GlyPro: 1.976 ± 0.055
2.415GlyGln: 2.415 ± 0.054
3.73GlyArg: 3.73 ± 0.068
4.578GlySer: 4.578 ± 0.09
4.212GlyThr: 4.212 ± 0.079
4.971GlyVal: 4.971 ± 0.092
0.905GlyTrp: 0.905 ± 0.034
2.804GlyTyr: 2.804 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.417HisAla: 1.417 ± 0.042
0.26HisCys: 0.26 ± 0.018
1.078HisAsp: 1.078 ± 0.036
1.32HisGlu: 1.32 ± 0.041
1.288HisPhe: 1.288 ± 0.036
1.578HisGly: 1.578 ± 0.043
0.724HisHis: 0.724 ± 0.033
1.465HisIle: 1.465 ± 0.04
0.944HisLys: 0.944 ± 0.033
2.533HisLeu: 2.533 ± 0.062
0.518HisMet: 0.518 ± 0.025
0.663HisAsn: 0.663 ± 0.03
1.441HisPro: 1.441 ± 0.042
0.956HisGln: 0.956 ± 0.035
1.142HisArg: 1.142 ± 0.039
1.302HisSer: 1.302 ± 0.04
1.033HisThr: 1.033 ± 0.035
1.643HisVal: 1.643 ± 0.043
0.29HisTrp: 0.29 ± 0.018
0.939HisTyr: 0.939 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
5.903IleAla: 5.903 ± 0.091
0.752IleCys: 0.752 ± 0.036
4.429IleAsp: 4.429 ± 0.079
4.554IleGlu: 4.554 ± 0.083
2.849IlePhe: 2.849 ± 0.076
6.01IleGly: 6.01 ± 0.104
1.767IleHis: 1.767 ± 0.047
5.247IleIle: 5.247 ± 0.1
4.161IleLys: 4.161 ± 0.076
6.652IleLeu: 6.652 ± 0.099
1.635IleMet: 1.635 ± 0.043
3.046IleAsn: 3.046 ± 0.059
3.397IlePro: 3.397 ± 0.066
2.587IleGln: 2.587 ± 0.058
4.142IleArg: 4.142 ± 0.072
4.685IleSer: 4.685 ± 0.082
3.737IleThr: 3.737 ± 0.068
5.124IleVal: 5.124 ± 0.085
0.618IleTrp: 0.618 ± 0.031
2.343IleTyr: 2.343 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.821LysAla: 4.821 ± 0.084
0.322LysCys: 0.322 ± 0.023
3.322LysAsp: 3.322 ± 0.068
4.972LysGlu: 4.972 ± 0.085
1.634LysPhe: 1.634 ± 0.046
4.099LysGly: 4.099 ± 0.064
1.183LysHis: 1.183 ± 0.041
4.613LysIle: 4.613 ± 0.087
5.987LysLys: 5.987 ± 0.094
5.365LysLeu: 5.365 ± 0.091
2.123LysMet: 2.123 ± 0.048
3.257LysAsn: 3.257 ± 0.067
2.309LysPro: 2.309 ± 0.062
2.589LysGln: 2.589 ± 0.058
3.472LysArg: 3.472 ± 0.061
3.481LysSer: 3.481 ± 0.064
3.702LysThr: 3.702 ± 0.07
4.398LysVal: 4.398 ± 0.067
0.704LysTrp: 0.704 ± 0.034
2.037LysTyr: 2.037 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
7.708LeuAla: 7.708 ± 0.088
0.746LeuCys: 0.746 ± 0.03
5.103LeuAsp: 5.103 ± 0.084
5.154LeuGlu: 5.154 ± 0.096
5.049LeuPhe: 5.049 ± 0.105
6.264LeuGly: 6.264 ± 0.101
1.933LeuHis: 1.933 ± 0.051
7.568LeuIle: 7.568 ± 0.119
6.559LeuLys: 6.559 ± 0.085
9.497LeuLeu: 9.497 ± 0.144
2.591LeuMet: 2.591 ± 0.06
4.441LeuAsn: 4.441 ± 0.074
4.198LeuPro: 4.198 ± 0.082
3.06LeuGln: 3.06 ± 0.059
4.37LeuArg: 4.37 ± 0.065
8.386LeuSer: 8.386 ± 0.203
5.592LeuThr: 5.592 ± 0.085
6.214LeuVal: 6.214 ± 0.098
0.813LeuTrp: 0.813 ± 0.033
3.088LeuTyr: 3.088 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.26MetAla: 2.26 ± 0.051
0.177MetCys: 0.177 ± 0.015
1.516MetAsp: 1.516 ± 0.044
1.559MetGlu: 1.559 ± 0.045
0.918MetPhe: 0.918 ± 0.032
1.805MetGly: 1.805 ± 0.045
0.5MetHis: 0.5 ± 0.026
2.487MetIle: 2.487 ± 0.06
2.116MetLys: 2.116 ± 0.051
2.265MetLeu: 2.265 ± 0.057
0.871MetMet: 0.871 ± 0.036
1.441MetAsn: 1.441 ± 0.042
1.118MetPro: 1.118 ± 0.043
0.806MetGln: 0.806 ± 0.035
1.222MetArg: 1.222 ± 0.038
1.686MetSer: 1.686 ± 0.044
1.788MetThr: 1.788 ± 0.046
1.757MetVal: 1.757 ± 0.045
0.163MetTrp: 0.163 ± 0.014
0.62MetTyr: 0.62 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.56AsnAla: 2.56 ± 0.059
0.32AsnCys: 0.32 ± 0.023
2.085AsnAsp: 2.085 ± 0.052
2.589AsnGlu: 2.589 ± 0.069
1.439AsnPhe: 1.439 ± 0.045
3.156AsnGly: 3.156 ± 0.075
1.249AsnHis: 1.249 ± 0.041
2.703AsnIle: 2.703 ± 0.053
2.535AsnLys: 2.535 ± 0.056
3.485AsnLeu: 3.485 ± 0.07
1.04AsnMet: 1.04 ± 0.037
1.583AsnAsn: 1.583 ± 0.052
2.157AsnPro: 2.157 ± 0.043
1.995AsnGln: 1.995 ± 0.053
2.471AsnArg: 2.471 ± 0.058
2.014AsnSer: 2.014 ± 0.063
1.744AsnThr: 1.744 ± 0.044
2.764AsnVal: 2.764 ± 0.057
0.461AsnTrp: 0.461 ± 0.024
1.46AsnTyr: 1.46 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
2.899ProAla: 2.899 ± 0.067
0.283ProCys: 0.283 ± 0.019
2.773ProAsp: 2.773 ± 0.06
3.403ProGlu: 3.403 ± 0.068
2.137ProPhe: 2.137 ± 0.058
3.253ProGly: 3.253 ± 0.092
0.794ProHis: 0.794 ± 0.03
2.614ProIle: 2.614 ± 0.057
2.073ProLys: 2.073 ± 0.061
3.786ProLeu: 3.786 ± 0.07
0.904ProMet: 0.904 ± 0.03
1.393ProAsn: 1.393 ± 0.046
1.146ProPro: 1.146 ± 0.047
1.159ProGln: 1.159 ± 0.039
1.372ProArg: 1.372 ± 0.047
2.391ProSer: 2.391 ± 0.053
1.718ProThr: 1.718 ± 0.052
3.596ProVal: 3.596 ± 0.073
0.407ProTrp: 0.407 ± 0.023
1.505ProTyr: 1.505 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
2.996GlnAla: 2.996 ± 0.059
0.197GlnCys: 0.197 ± 0.015
1.53GlnAsp: 1.53 ± 0.042
2.152GlnGlu: 2.152 ± 0.055
1.832GlnPhe: 1.832 ± 0.078
2.117GlnGly: 2.117 ± 0.058
0.681GlnHis: 0.681 ± 0.028
2.729GlnIle: 2.729 ± 0.059
2.782GlnLys: 2.782 ± 0.069
4.969GlnLeu: 4.969 ± 0.21
1.26GlnMet: 1.26 ± 0.035
1.571GlnAsn: 1.571 ± 0.048
1.352GlnPro: 1.352 ± 0.049
1.342GlnGln: 1.342 ± 0.049
1.63GlnArg: 1.63 ± 0.046
2.308GlnSer: 2.308 ± 0.054
2.085GlnThr: 2.085 ± 0.048
2.462GlnVal: 2.462 ± 0.058
0.392GlnTrp: 0.392 ± 0.026
1.082GlnTyr: 1.082 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.196ArgAla: 3.196 ± 0.061
0.383ArgCys: 0.383 ± 0.023
2.567ArgAsp: 2.567 ± 0.068
3.634ArgGlu: 3.634 ± 0.073
2.565ArgPhe: 2.565 ± 0.069
2.86ArgGly: 2.86 ± 0.072
1.207ArgHis: 1.207 ± 0.042
3.633ArgIle: 3.633 ± 0.064
3.854ArgLys: 3.854 ± 0.065
5.682ArgLeu: 5.682 ± 0.089
1.602ArgMet: 1.602 ± 0.04
2.048ArgAsn: 2.048 ± 0.05
1.917ArgPro: 1.917 ± 0.043
3.749ArgGln: 3.749 ± 0.267
3.08ArgArg: 3.08 ± 0.071
3.236ArgSer: 3.236 ± 0.087
2.689ArgThr: 2.689 ± 0.061
3.288ArgVal: 3.288 ± 0.065
0.51ArgTrp: 0.51 ± 0.025
1.842ArgTyr: 1.842 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.427SerAla: 4.427 ± 0.081
0.663SerCys: 0.663 ± 0.046
3.372SerAsp: 3.372 ± 0.072
3.783SerGlu: 3.783 ± 0.075
3.18SerPhe: 3.18 ± 0.071
5.613SerGly: 5.613 ± 0.095
1.386SerHis: 1.386 ± 0.041
4.531SerIle: 4.531 ± 0.073
3.25SerLys: 3.25 ± 0.073
6.483SerLeu: 6.483 ± 0.088
1.661SerMet: 1.661 ± 0.045
2.038SerAsn: 2.038 ± 0.052
2.439SerPro: 2.439 ± 0.057
1.881SerGln: 1.881 ± 0.046
3.881SerArg: 3.881 ± 0.123
4.47SerSer: 4.47 ± 0.125
2.891SerThr: 2.891 ± 0.055
4.595SerVal: 4.595 ± 0.076
0.654SerTrp: 0.654 ± 0.031
2.54SerTyr: 2.54 ± 0.125
0.0SerXaa: 0.0 ± 0.0
Thr
4.251ThrAla: 4.251 ± 0.073
0.478ThrCys: 0.478 ± 0.026
2.901ThrAsp: 2.901 ± 0.059
3.027ThrGlu: 3.027 ± 0.061
2.476ThrPhe: 2.476 ± 0.056
4.805ThrGly: 4.805 ± 0.094
1.099ThrHis: 1.099 ± 0.038
3.932ThrIle: 3.932 ± 0.068
2.628ThrLys: 2.628 ± 0.061
5.356ThrLeu: 5.356 ± 0.075
1.228ThrMet: 1.228 ± 0.039
1.916ThrAsn: 1.916 ± 0.054
2.299ThrPro: 2.299 ± 0.055
1.392ThrGln: 1.392 ± 0.039
2.354ThrArg: 2.354 ± 0.05
3.01ThrSer: 3.01 ± 0.061
2.601ThrThr: 2.601 ± 0.067
4.37ThrVal: 4.37 ± 0.073
0.451ThrTrp: 0.451 ± 0.024
1.763ThrTyr: 1.763 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
4.829ValAla: 4.829 ± 0.076
0.725ValCys: 0.725 ± 0.029
3.57ValAsp: 3.57 ± 0.067
3.701ValGlu: 3.701 ± 0.072
3.089ValPhe: 3.089 ± 0.06
4.387ValGly: 4.387 ± 0.077
1.587ValHis: 1.587 ± 0.043
5.728ValIle: 5.728 ± 0.099
4.161ValLys: 4.161 ± 0.061
6.785ValLeu: 6.785 ± 0.09
1.832ValMet: 1.832 ± 0.052
2.846ValAsn: 2.846 ± 0.06
3.088ValPro: 3.088 ± 0.059
2.337ValGln: 2.337 ± 0.053
4.008ValArg: 4.008 ± 0.094
4.883ValSer: 4.883 ± 0.072
4.271ValThr: 4.271 ± 0.077
4.671ValVal: 4.671 ± 0.089
0.632ValTrp: 0.632 ± 0.028
2.274ValTyr: 2.274 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.636TrpAla: 0.636 ± 0.03
0.075TrpCys: 0.075 ± 0.009
0.503TrpAsp: 0.503 ± 0.023
0.498TrpGlu: 0.498 ± 0.027
0.491TrpPhe: 0.491 ± 0.026
0.631TrpGly: 0.631 ± 0.028
0.242TrpHis: 0.242 ± 0.016
0.803TrpIle: 0.803 ± 0.04
0.651TrpLys: 0.651 ± 0.026
1.218TrpLeu: 1.218 ± 0.042
0.341TrpMet: 0.341 ± 0.02
0.483TrpAsn: 0.483 ± 0.021
0.31TrpPro: 0.31 ± 0.021
0.383TrpGln: 0.383 ± 0.022
0.491TrpArg: 0.491 ± 0.026
0.651TrpSer: 0.651 ± 0.037
0.551TrpThr: 0.551 ± 0.024
0.618TrpVal: 0.618 ± 0.03
0.124TrpTrp: 0.124 ± 0.01
0.358TrpTyr: 0.358 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.155TyrAla: 2.155 ± 0.05
0.345TyrCys: 0.345 ± 0.021
1.815TyrAsp: 1.815 ± 0.045
1.866TyrGlu: 1.866 ± 0.049
1.657TyrPhe: 1.657 ± 0.044
2.493TyrGly: 2.493 ± 0.06
0.956TyrHis: 0.956 ± 0.032
2.165TyrIle: 2.165 ± 0.052
1.76TyrLys: 1.76 ± 0.054
3.567TyrLeu: 3.567 ± 0.074
0.731TyrMet: 0.731 ± 0.029
1.263TyrAsn: 1.263 ± 0.045
1.488TyrPro: 1.488 ± 0.049
1.478TyrGln: 1.478 ± 0.05
3.097TyrArg: 3.097 ± 0.219
1.982TyrSer: 1.982 ± 0.058
1.732TyrThr: 1.732 ± 0.047
2.091TyrVal: 2.091 ± 0.058
0.396TyrTrp: 0.396 ± 0.02
1.325TyrTyr: 1.325 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2760 proteins (852718 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski