Streptococcus phage Javan363

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A805|A0A4D6A805_9CAUD Terminase small subunit OS=Streptococcus phage Javan363 OX=2548126 GN=Javan363_0020 PE=4 SV=1
MM1 pKa = 7.35IPKK4 pKa = 9.71FRR6 pKa = 11.84AWDD9 pKa = 3.48KK10 pKa = 11.3ARR12 pKa = 11.84NEE14 pKa = 3.98MNYY17 pKa = 10.54KK18 pKa = 10.52VMVGNCDD25 pKa = 3.32TDD27 pKa = 3.87DD28 pKa = 4.79KK29 pKa = 11.7NWTCPIVWIEE39 pKa = 3.96EE40 pKa = 4.19KK41 pKa = 10.6KK42 pKa = 10.73DD43 pKa = 3.08WLHH46 pKa = 6.79FDD48 pKa = 4.07DD49 pKa = 5.62YY50 pKa = 11.11EE51 pKa = 6.52CIMQSTGLEE60 pKa = 3.85DD61 pKa = 4.97DD62 pKa = 3.92FGKK65 pKa = 10.75EE66 pKa = 3.5IFEE69 pKa = 4.52GDD71 pKa = 3.93VILWTYY77 pKa = 9.1WDD79 pKa = 3.75EE80 pKa = 4.71FEE82 pKa = 5.6DD83 pKa = 3.61SGRR86 pKa = 11.84AKK88 pKa = 10.76VIFDD92 pKa = 3.24NGMFRR97 pKa = 11.84LLDD100 pKa = 3.17IRR102 pKa = 11.84TEE104 pKa = 4.06KK105 pKa = 10.65EE106 pKa = 3.64VWDD109 pKa = 4.0NLFDD113 pKa = 5.56CIEE116 pKa = 4.12NCNVYY121 pKa = 10.51LQGNVYY127 pKa = 10.07EE128 pKa = 4.35NPEE131 pKa = 4.02LLEE134 pKa = 4.19VKK136 pKa = 10.04EE137 pKa = 4.2

Molecular weight: 16.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A960|A0A4D6A960_9CAUD DNA-binding protein OS=Streptococcus phage Javan363 OX=2548126 GN=Javan363_0033 PE=4 SV=1
MM1 pKa = 8.04AMRR4 pKa = 11.84PKK6 pKa = 10.78KK7 pKa = 10.21NIRR10 pKa = 11.84FQAPQIEE17 pKa = 4.54KK18 pKa = 10.85GFIDD22 pKa = 5.0FKK24 pKa = 9.71TGRR27 pKa = 11.84RR28 pKa = 11.84VDD30 pKa = 3.52INPVTRR36 pKa = 11.84KK37 pKa = 7.79EE38 pKa = 4.44TFVDD42 pKa = 3.56

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

8654

38

1138

196.7

22.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.621 ± 0.536

0.439 ± 0.117

5.905 ± 0.454

8.216 ± 0.542

4.125 ± 0.334

6.147 ± 0.295

1.433 ± 0.185

7.546 ± 0.416

8.158 ± 0.41

8.412 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.294

5.859 ± 0.302

2.75 ± 0.258

4.252 ± 0.374

4.53 ± 0.388

6.124 ± 0.385

5.939 ± 0.443

5.847 ± 0.276

1.421 ± 0.221

3.64 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski