Amino acid dipepetide frequency for Streptococcus phage Javan363

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.276AlaAla: 4.276 ± 0.977
0.231AlaCys: 0.231 ± 0.152
4.623AlaAsp: 4.623 ± 0.659
4.507AlaGlu: 4.507 ± 0.598
2.427AlaPhe: 2.427 ± 0.431
3.698AlaGly: 3.698 ± 0.637
0.578AlaHis: 0.578 ± 0.203
6.472AlaIle: 6.472 ± 1.184
4.276AlaLys: 4.276 ± 0.668
7.859AlaLeu: 7.859 ± 1.202
2.311AlaMet: 2.311 ± 0.456
3.583AlaAsn: 3.583 ± 0.923
1.271AlaPro: 1.271 ± 0.282
3.236AlaGln: 3.236 ± 1.012
2.889AlaArg: 2.889 ± 0.705
4.045AlaSer: 4.045 ± 0.77
2.658AlaThr: 2.658 ± 0.571
3.698AlaVal: 3.698 ± 0.53
0.925AlaTrp: 0.925 ± 0.369
2.658AlaTyr: 2.658 ± 0.58
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.347CysAsp: 0.347 ± 0.219
0.462CysGlu: 0.462 ± 0.269
0.0CysPhe: 0.0 ± 0.0
0.116CysGly: 0.116 ± 0.121
0.116CysHis: 0.116 ± 0.128
0.693CysIle: 0.693 ± 0.42
0.231CysLys: 0.231 ± 0.174
0.231CysLeu: 0.231 ± 0.156
0.0CysMet: 0.0 ± 0.0
0.347CysAsn: 0.347 ± 0.229
0.116CysPro: 0.116 ± 0.145
0.231CysGln: 0.231 ± 0.135
0.116CysArg: 0.116 ± 0.102
0.462CysSer: 0.462 ± 0.196
0.347CysThr: 0.347 ± 0.241
0.116CysVal: 0.116 ± 0.125
0.116CysTrp: 0.116 ± 0.121
0.347CysTyr: 0.347 ± 0.22
0.0CysXaa: 0.0 ± 0.0
Asp
2.889AspAla: 2.889 ± 0.561
0.231AspCys: 0.231 ± 0.209
2.774AspAsp: 2.774 ± 0.798
4.738AspGlu: 4.738 ± 1.055
3.005AspPhe: 3.005 ± 0.789
5.201AspGly: 5.201 ± 1.131
0.693AspHis: 0.693 ± 0.279
6.356AspIle: 6.356 ± 0.732
3.929AspLys: 3.929 ± 0.496
4.392AspLeu: 4.392 ± 0.637
1.849AspMet: 1.849 ± 0.409
3.236AspAsn: 3.236 ± 0.685
1.965AspPro: 1.965 ± 0.614
1.502AspGln: 1.502 ± 0.427
2.196AspArg: 2.196 ± 0.56
3.351AspSer: 3.351 ± 0.567
3.351AspThr: 3.351 ± 0.534
3.583AspVal: 3.583 ± 0.554
0.809AspTrp: 0.809 ± 0.228
3.005AspTyr: 3.005 ± 0.545
0.0AspXaa: 0.0 ± 0.0
Glu
5.085GluAla: 5.085 ± 0.844
0.116GluCys: 0.116 ± 0.145
3.698GluAsp: 3.698 ± 0.906
8.321GluGlu: 8.321 ± 1.542
2.889GluPhe: 2.889 ± 0.739
3.467GluGly: 3.467 ± 0.58
0.809GluHis: 0.809 ± 0.295
6.241GluIle: 6.241 ± 0.948
6.818GluLys: 6.818 ± 0.934
9.592GluLeu: 9.592 ± 1.406
3.236GluMet: 3.236 ± 0.771
5.085GluAsn: 5.085 ± 0.7
1.156GluPro: 1.156 ± 0.461
4.16GluGln: 4.16 ± 0.728
4.392GluArg: 4.392 ± 0.774
3.698GluSer: 3.698 ± 0.674
4.16GluThr: 4.16 ± 0.781
5.316GluVal: 5.316 ± 0.933
1.271GluTrp: 1.271 ± 0.402
2.658GluTyr: 2.658 ± 0.613
0.0GluXaa: 0.0 ± 0.0
Phe
2.311PheAla: 2.311 ± 0.51
0.0PheCys: 0.0 ± 0.0
2.658PheAsp: 2.658 ± 0.705
2.774PheGlu: 2.774 ± 0.699
1.618PhePhe: 1.618 ± 0.55
3.814PheGly: 3.814 ± 0.632
0.231PheHis: 0.231 ± 0.16
4.045PheIle: 4.045 ± 0.79
4.738PheLys: 4.738 ± 0.602
2.889PheLeu: 2.889 ± 0.604
1.271PheMet: 1.271 ± 0.415
2.774PheAsn: 2.774 ± 0.562
0.925PhePro: 0.925 ± 0.382
1.271PheGln: 1.271 ± 0.367
1.502PheArg: 1.502 ± 0.458
2.658PheSer: 2.658 ± 0.488
1.734PheThr: 1.734 ± 0.371
2.08PheVal: 2.08 ± 0.581
0.462PheTrp: 0.462 ± 0.218
1.502PheTyr: 1.502 ± 0.485
0.0PheXaa: 0.0 ± 0.0
Gly
4.507GlyAla: 4.507 ± 0.922
0.231GlyCys: 0.231 ± 0.148
5.085GlyAsp: 5.085 ± 0.773
3.698GlyGlu: 3.698 ± 0.642
1.849GlyPhe: 1.849 ± 0.637
3.467GlyGly: 3.467 ± 0.604
0.809GlyHis: 0.809 ± 0.365
4.854GlyIle: 4.854 ± 0.88
4.854GlyLys: 4.854 ± 0.632
5.547GlyLeu: 5.547 ± 0.853
1.618GlyMet: 1.618 ± 0.453
4.045GlyAsn: 4.045 ± 0.816
0.578GlyPro: 0.578 ± 0.219
2.08GlyGln: 2.08 ± 0.467
2.658GlyArg: 2.658 ± 0.481
3.698GlySer: 3.698 ± 0.666
4.045GlyThr: 4.045 ± 0.458
3.467GlyVal: 3.467 ± 0.493
1.387GlyTrp: 1.387 ± 0.704
3.005GlyTyr: 3.005 ± 0.65
0.0GlyXaa: 0.0 ± 0.0
His
0.462HisAla: 0.462 ± 0.179
0.116HisCys: 0.116 ± 0.125
0.809HisAsp: 0.809 ± 0.334
0.809HisGlu: 0.809 ± 0.32
1.271HisPhe: 1.271 ± 0.33
0.347HisGly: 0.347 ± 0.231
0.116HisHis: 0.116 ± 0.126
1.618HisIle: 1.618 ± 0.476
0.693HisLys: 0.693 ± 0.349
0.809HisLeu: 0.809 ± 0.252
0.462HisMet: 0.462 ± 0.2
0.693HisAsn: 0.693 ± 0.328
0.347HisPro: 0.347 ± 0.229
0.925HisGln: 0.925 ± 0.324
0.693HisArg: 0.693 ± 0.249
1.387HisSer: 1.387 ± 0.388
1.271HisThr: 1.271 ± 0.286
1.04HisVal: 1.04 ± 0.353
0.0HisTrp: 0.0 ± 0.0
0.462HisTyr: 0.462 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
4.623IleAla: 4.623 ± 0.736
0.231IleCys: 0.231 ± 0.249
3.929IleAsp: 3.929 ± 0.638
6.125IleGlu: 6.125 ± 0.907
2.658IlePhe: 2.658 ± 0.584
4.276IleGly: 4.276 ± 0.919
1.387IleHis: 1.387 ± 0.359
5.201IleIle: 5.201 ± 0.904
7.627IleLys: 7.627 ± 0.908
4.854IleLeu: 4.854 ± 0.766
2.08IleMet: 2.08 ± 0.472
4.276IleAsn: 4.276 ± 0.817
2.196IlePro: 2.196 ± 0.371
3.929IleGln: 3.929 ± 0.548
3.467IleArg: 3.467 ± 0.597
6.356IleSer: 6.356 ± 1.332
5.201IleThr: 5.201 ± 0.804
4.16IleVal: 4.16 ± 0.816
0.347IleTrp: 0.347 ± 0.255
2.542IleTyr: 2.542 ± 0.495
0.0IleXaa: 0.0 ± 0.0
Lys
4.738LysAla: 4.738 ± 0.723
0.116LysCys: 0.116 ± 0.125
5.778LysAsp: 5.778 ± 0.839
8.09LysGlu: 8.09 ± 1.1
2.542LysPhe: 2.542 ± 0.509
3.814LysGly: 3.814 ± 0.74
1.271LysHis: 1.271 ± 0.476
5.316LysIle: 5.316 ± 0.584
7.859LysLys: 7.859 ± 0.985
5.316LysLeu: 5.316 ± 0.653
1.618LysMet: 1.618 ± 0.472
6.125LysAsn: 6.125 ± 0.738
2.889LysPro: 2.889 ± 0.534
3.583LysGln: 3.583 ± 0.667
4.16LysArg: 4.16 ± 1.012
5.085LysSer: 5.085 ± 0.791
5.778LysThr: 5.778 ± 0.875
4.276LysVal: 4.276 ± 0.707
1.156LysTrp: 1.156 ± 0.367
2.08LysTyr: 2.08 ± 0.435
0.0LysXaa: 0.0 ± 0.0
Leu
6.241LeuAla: 6.241 ± 0.935
0.347LeuCys: 0.347 ± 0.194
5.316LeuAsp: 5.316 ± 0.624
8.321LeuGlu: 8.321 ± 1.42
3.814LeuPhe: 3.814 ± 0.745
5.547LeuGly: 5.547 ± 0.742
1.387LeuHis: 1.387 ± 0.376
4.623LeuIle: 4.623 ± 0.832
7.974LeuLys: 7.974 ± 0.851
6.241LeuLeu: 6.241 ± 1.117
1.965LeuMet: 1.965 ± 0.412
4.392LeuAsn: 4.392 ± 0.748
2.542LeuPro: 2.542 ± 0.459
3.005LeuGln: 3.005 ± 0.773
4.16LeuArg: 4.16 ± 0.872
4.854LeuSer: 4.854 ± 0.763
5.778LeuThr: 5.778 ± 0.662
4.045LeuVal: 4.045 ± 0.686
0.925LeuTrp: 0.925 ± 0.293
2.658LeuTyr: 2.658 ± 0.474
0.0LeuXaa: 0.0 ± 0.0
Met
1.734MetAla: 1.734 ± 0.337
0.0MetCys: 0.0 ± 0.0
1.502MetAsp: 1.502 ± 0.44
2.427MetGlu: 2.427 ± 0.657
1.387MetPhe: 1.387 ± 0.394
0.925MetGly: 0.925 ± 0.299
0.116MetHis: 0.116 ± 0.112
1.734MetIle: 1.734 ± 0.476
1.849MetLys: 1.849 ± 0.541
1.618MetLeu: 1.618 ± 0.514
0.693MetMet: 0.693 ± 0.275
1.849MetAsn: 1.849 ± 0.501
0.693MetPro: 0.693 ± 0.318
1.04MetGln: 1.04 ± 0.284
1.502MetArg: 1.502 ± 0.423
2.889MetSer: 2.889 ± 0.662
2.196MetThr: 2.196 ± 0.519
1.734MetVal: 1.734 ± 0.392
0.347MetTrp: 0.347 ± 0.172
0.116MetTyr: 0.116 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
4.276AsnAla: 4.276 ± 0.786
0.462AsnCys: 0.462 ± 0.34
2.889AsnAsp: 2.889 ± 0.602
4.507AsnGlu: 4.507 ± 0.492
2.196AsnPhe: 2.196 ± 0.402
5.085AsnGly: 5.085 ± 0.826
1.502AsnHis: 1.502 ± 0.452
2.774AsnIle: 2.774 ± 0.639
4.16AsnLys: 4.16 ± 0.641
5.316AsnLeu: 5.316 ± 0.827
0.462AsnMet: 0.462 ± 0.308
2.427AsnAsn: 2.427 ± 0.549
3.236AsnPro: 3.236 ± 0.672
4.045AsnGln: 4.045 ± 0.783
2.08AsnArg: 2.08 ± 0.485
3.005AsnSer: 3.005 ± 0.577
3.236AsnThr: 3.236 ± 0.43
4.507AsnVal: 4.507 ± 0.828
0.693AsnTrp: 0.693 ± 0.277
1.618AsnTyr: 1.618 ± 0.563
0.0AsnXaa: 0.0 ± 0.0
Pro
1.618ProAla: 1.618 ± 0.512
0.347ProCys: 0.347 ± 0.19
1.734ProAsp: 1.734 ± 0.786
2.427ProGlu: 2.427 ± 0.514
0.925ProPhe: 0.925 ± 0.382
1.271ProGly: 1.271 ± 0.369
0.578ProHis: 0.578 ± 0.247
2.658ProIle: 2.658 ± 0.655
1.965ProLys: 1.965 ± 0.562
1.965ProLeu: 1.965 ± 0.373
0.462ProMet: 0.462 ± 0.244
0.578ProAsn: 0.578 ± 0.26
0.347ProPro: 0.347 ± 0.208
1.618ProGln: 1.618 ± 0.426
0.925ProArg: 0.925 ± 0.281
2.196ProSer: 2.196 ± 0.545
2.196ProThr: 2.196 ± 0.491
1.965ProVal: 1.965 ± 0.427
0.231ProTrp: 0.231 ± 0.16
1.502ProTyr: 1.502 ± 0.413
0.0ProXaa: 0.0 ± 0.0
Gln
5.547GlnAla: 5.547 ± 1.039
0.116GlnCys: 0.116 ± 0.108
1.965GlnAsp: 1.965 ± 0.49
3.583GlnGlu: 3.583 ± 0.702
1.734GlnPhe: 1.734 ± 0.444
1.271GlnGly: 1.271 ± 0.467
0.231GlnHis: 0.231 ± 0.135
2.658GlnIle: 2.658 ± 0.524
3.351GlnLys: 3.351 ± 0.749
3.583GlnLeu: 3.583 ± 0.626
1.618GlnMet: 1.618 ± 0.422
2.889GlnAsn: 2.889 ± 0.527
1.271GlnPro: 1.271 ± 0.413
1.387GlnGln: 1.387 ± 0.421
1.965GlnArg: 1.965 ± 0.534
2.774GlnSer: 2.774 ± 0.425
2.658GlnThr: 2.658 ± 0.531
2.542GlnVal: 2.542 ± 0.533
0.347GlnTrp: 0.347 ± 0.216
1.04GlnTyr: 1.04 ± 0.273
0.0GlnXaa: 0.0 ± 0.0
Arg
3.236ArgAla: 3.236 ± 0.549
0.347ArgCys: 0.347 ± 0.243
1.965ArgAsp: 1.965 ± 0.733
4.045ArgGlu: 4.045 ± 0.79
1.618ArgPhe: 1.618 ± 0.479
2.427ArgGly: 2.427 ± 0.7
0.693ArgHis: 0.693 ± 0.317
3.698ArgIle: 3.698 ± 0.782
3.351ArgLys: 3.351 ± 0.841
4.276ArgLeu: 4.276 ± 0.859
1.04ArgMet: 1.04 ± 0.355
3.351ArgAsn: 3.351 ± 0.672
1.618ArgPro: 1.618 ± 0.367
1.618ArgGln: 1.618 ± 0.448
2.542ArgArg: 2.542 ± 0.686
2.311ArgSer: 2.311 ± 0.705
1.965ArgThr: 1.965 ± 0.556
1.965ArgVal: 1.965 ± 0.428
1.04ArgTrp: 1.04 ± 0.361
2.196ArgTyr: 2.196 ± 0.656
0.0ArgXaa: 0.0 ± 0.0
Ser
3.929SerAla: 3.929 ± 0.729
0.231SerCys: 0.231 ± 0.151
4.623SerAsp: 4.623 ± 1.015
3.351SerGlu: 3.351 ± 0.637
3.929SerPhe: 3.929 ± 0.59
5.432SerGly: 5.432 ± 0.778
0.693SerHis: 0.693 ± 0.301
3.929SerIle: 3.929 ± 0.864
4.045SerLys: 4.045 ± 0.625
4.854SerLeu: 4.854 ± 0.691
1.965SerMet: 1.965 ± 0.568
3.814SerAsn: 3.814 ± 0.642
1.502SerPro: 1.502 ± 0.462
2.196SerGln: 2.196 ± 0.664
2.658SerArg: 2.658 ± 0.45
2.889SerSer: 2.889 ± 0.605
4.045SerThr: 4.045 ± 0.99
3.583SerVal: 3.583 ± 0.508
0.925SerTrp: 0.925 ± 0.294
2.658SerTyr: 2.658 ± 0.472
0.0SerXaa: 0.0 ± 0.0
Thr
4.969ThrAla: 4.969 ± 0.985
0.347ThrCys: 0.347 ± 0.215
3.005ThrAsp: 3.005 ± 0.865
4.392ThrGlu: 4.392 ± 0.598
2.542ThrPhe: 2.542 ± 0.669
4.045ThrGly: 4.045 ± 0.643
1.387ThrHis: 1.387 ± 0.354
4.969ThrIle: 4.969 ± 0.88
4.392ThrLys: 4.392 ± 0.61
5.663ThrLeu: 5.663 ± 0.662
0.925ThrMet: 0.925 ± 0.404
2.658ThrAsn: 2.658 ± 0.413
1.04ThrPro: 1.04 ± 0.291
2.196ThrGln: 2.196 ± 0.575
2.774ThrArg: 2.774 ± 0.516
3.467ThrSer: 3.467 ± 0.583
3.12ThrThr: 3.12 ± 0.706
4.507ThrVal: 4.507 ± 1.085
0.925ThrTrp: 0.925 ± 0.436
2.08ThrTyr: 2.08 ± 0.601
0.0ThrXaa: 0.0 ± 0.0
Val
3.929ValAla: 3.929 ± 0.742
0.116ValCys: 0.116 ± 0.119
3.698ValAsp: 3.698 ± 0.781
4.623ValGlu: 4.623 ± 0.798
1.502ValPhe: 1.502 ± 0.537
4.623ValGly: 4.623 ± 0.62
1.04ValHis: 1.04 ± 0.302
4.16ValIle: 4.16 ± 0.874
4.623ValLys: 4.623 ± 1.018
4.738ValLeu: 4.738 ± 0.671
1.502ValMet: 1.502 ± 0.352
3.698ValAsn: 3.698 ± 0.646
1.387ValPro: 1.387 ± 0.412
2.658ValGln: 2.658 ± 0.637
1.502ValArg: 1.502 ± 0.461
3.929ValSer: 3.929 ± 0.658
4.045ValThr: 4.045 ± 0.672
3.929ValVal: 3.929 ± 0.728
1.387ValTrp: 1.387 ± 0.734
1.387ValTyr: 1.387 ± 0.567
0.0ValXaa: 0.0 ± 0.0
Trp
0.462TrpAla: 0.462 ± 0.216
0.231TrpCys: 0.231 ± 0.17
0.462TrpAsp: 0.462 ± 0.463
1.387TrpGlu: 1.387 ± 0.584
0.809TrpPhe: 0.809 ± 0.386
0.693TrpGly: 0.693 ± 0.251
0.116TrpHis: 0.116 ± 0.126
1.271TrpIle: 1.271 ± 0.433
1.618TrpLys: 1.618 ± 0.414
0.809TrpLeu: 0.809 ± 0.349
0.347TrpMet: 0.347 ± 0.161
1.271TrpAsn: 1.271 ± 0.349
0.347TrpPro: 0.347 ± 0.187
0.578TrpGln: 0.578 ± 0.314
0.693TrpArg: 0.693 ± 0.258
0.809TrpSer: 0.809 ± 0.253
0.693TrpThr: 0.693 ± 0.386
0.462TrpVal: 0.462 ± 0.207
0.462TrpTrp: 0.462 ± 0.286
0.693TrpTyr: 0.693 ± 0.464
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.387TyrAla: 1.387 ± 0.358
0.578TyrCys: 0.578 ± 0.32
2.196TyrAsp: 2.196 ± 0.468
3.583TyrGlu: 3.583 ± 0.749
2.542TyrPhe: 2.542 ± 0.492
1.965TyrGly: 1.965 ± 0.502
0.578TyrHis: 0.578 ± 0.205
2.196TyrIle: 2.196 ± 0.647
3.236TyrLys: 3.236 ± 0.634
3.583TyrLeu: 3.583 ± 0.605
0.809TyrMet: 0.809 ± 0.334
1.502TyrAsn: 1.502 ± 0.403
2.08TyrPro: 2.08 ± 0.544
1.271TyrGln: 1.271 ± 0.389
2.427TyrArg: 2.427 ± 0.461
1.387TyrSer: 1.387 ± 0.416
0.925TyrThr: 0.925 ± 0.329
1.502TyrVal: 1.502 ± 0.342
0.462TyrTrp: 0.462 ± 0.232
2.196TyrTyr: 2.196 ± 0.653
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (8654 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski