Sporosarcina luteola
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3572 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511Z532|A0A511Z532_9BACL Sulfur carrier protein FdhD OS=Sporosarcina luteola OX=582850 GN=fdhD PE=3 SV=1
MM1 pKa = 7.64 SNTTRR6 pKa = 11.84 PGRR9 pKa = 11.84 NDD11 pKa = 3.31 APICEE16 pKa = 4.85 LLMNLPPLTALTEE29 pKa = 4.27 VVVDD33 pKa = 5.26 GIPFAVTSFININSDD48 pKa = 3.2 TNLAYY53 pKa = 10.35 FLSTVGTTLIVDD65 pKa = 4.54 CDD67 pKa = 3.89 SLSQVSFF74 pKa = 3.73
Molecular weight: 7.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.821
IPC_protein 3.643
Toseland 3.452
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.617
Lehninger 3.579
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.783
Patrickios 1.888
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A511ZCC9|A0A511ZCC9_9BACL 33 kDa chaperonin OS=Sporosarcina luteola OX=582850 GN=hslO PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPNKK9 pKa = 8.24 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 10.14 SGRR28 pKa = 11.84 NILAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.84 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3572
0
3572
1034920
39
2038
289.7
32.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.5 ± 0.043
0.612 ± 0.011
5.41 ± 0.037
7.726 ± 0.052
4.584 ± 0.031
7.08 ± 0.044
2.007 ± 0.02
7.681 ± 0.047
6.405 ± 0.036
9.589 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.049 ± 0.018
4.043 ± 0.028
3.619 ± 0.024
3.403 ± 0.024
4.297 ± 0.032
5.955 ± 0.026
5.593 ± 0.036
7.174 ± 0.03
0.981 ± 0.014
3.293 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here