Betaproteobacteria bacterium SCN1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H9Y4A2|A0A6H9Y4A2_9PROT UPF0391 membrane protein F8A86_07025 OS=Betaproteobacteria bacterium SCN1 OX=2651627 GN=F8A86_07025 PE=3 SV=1
MM1 pKa = 7.09QSKK4 pKa = 10.6SLILAVALACAGTAAQAAVPDD25 pKa = 4.01YY26 pKa = 10.02MPLGSGTYY34 pKa = 9.47LGSVGADD41 pKa = 3.12THH43 pKa = 5.73GTPANWSFWRR53 pKa = 11.84FNVDD57 pKa = 4.14FLDD60 pKa = 4.04TATLTVTPGPTDD72 pKa = 3.63FDD74 pKa = 4.13VVFGVWYY81 pKa = 10.29GLEE84 pKa = 4.25SDD86 pKa = 3.63TANYY90 pKa = 10.0FDD92 pKa = 5.29LFSGAAASSVFVGLGDD108 pKa = 3.9GTDD111 pKa = 3.32PWTVGPGVPATFSFTNTYY129 pKa = 11.01GNDD132 pKa = 3.62DD133 pKa = 4.18FVLAIADD140 pKa = 3.85YY141 pKa = 10.08TDD143 pKa = 3.69EE144 pKa = 5.11LGSGMMTYY152 pKa = 9.8TLTATVPEE160 pKa = 4.45AEE162 pKa = 4.31TWGLMLAGLGLVGLAARR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84AA183 pKa = 2.98

Molecular weight: 19.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H9YF17|A0A6H9YF17_9PROT Uncharacterized protein OS=Betaproteobacteria bacterium SCN1 OX=2651627 GN=F8A86_00115 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.29QPSTVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.38RR14 pKa = 11.84THH16 pKa = 5.85GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.62TKK25 pKa = 10.49AGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.07GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3025

0

3025

953606

37

3408

315.2

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.958 ± 0.067

0.928 ± 0.014

5.736 ± 0.033

5.371 ± 0.046

3.53 ± 0.028

8.192 ± 0.058

2.325 ± 0.024

4.489 ± 0.032

3.186 ± 0.043

11.019 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.025

2.62 ± 0.029

5.291 ± 0.037

3.544 ± 0.026

7.322 ± 0.046

4.763 ± 0.035

5.106 ± 0.031

7.299 ± 0.042

1.464 ± 0.023

2.459 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski