Pseudomonas saudiphocaensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078LU91|A0A078LU91_9PSED Uncharacterized protein OS=Pseudomonas saudiphocaensis OX=1499686 GN=BN1079_03338 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 3.01IPSDD7 pKa = 3.47LRR9 pKa = 11.84YY10 pKa = 9.66AASHH14 pKa = 5.24EE15 pKa = 4.31WARR18 pKa = 11.84QEE20 pKa = 4.0ADD22 pKa = 3.0GSVSVGISDD31 pKa = 4.17HH32 pKa = 6.12AQEE35 pKa = 4.34ALGDD39 pKa = 3.84VVYY42 pKa = 11.28VEE44 pKa = 5.05LPQVGQQFQAGQQVGVVEE62 pKa = 4.47SVKK65 pKa = 10.57AASDD69 pKa = 3.41IYY71 pKa = 11.12APVSGVVTAVNEE83 pKa = 4.15QLVDD87 pKa = 3.45APEE90 pKa = 4.11QVNSDD95 pKa = 3.96PYY97 pKa = 11.08GSWFYY102 pKa = 11.17RR103 pKa = 11.84LQPDD107 pKa = 4.1TPAEE111 pKa = 4.04LEE113 pKa = 4.16ALLSADD119 pKa = 4.31AYY121 pKa = 10.05KK122 pKa = 10.75ASCDD126 pKa = 3.34AA127 pKa = 4.84

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078M0D2|A0A078M0D2_9PSED Uncharacterized protein OS=Pseudomonas saudiphocaensis OX=1499686 GN=BN1079_03477 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.48RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.33GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3411

0

3411

1093476

30

2385

320.6

35.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.882 ± 0.044

1.002 ± 0.016

5.286 ± 0.028

6.377 ± 0.043

3.593 ± 0.028

7.902 ± 0.037

2.236 ± 0.02

4.901 ± 0.032

3.258 ± 0.033

12.007 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.023

2.918 ± 0.022

4.787 ± 0.029

4.571 ± 0.035

6.829 ± 0.046

5.635 ± 0.03

4.636 ± 0.026

6.934 ± 0.038

1.386 ± 0.018

2.458 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski