Staphylococcus phage vB_SauS_IMEP5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D6Z282|A0A1D6Z282_9CAUD Tail fiber OS=Staphylococcus phage vB_SauS_IMEP5 OX=1852565 PE=4 SV=1
MM1 pKa = 7.51AMSNDD6 pKa = 2.92IFPPFSTIKK15 pKa = 10.65YY16 pKa = 9.53LYY18 pKa = 10.41DD19 pKa = 3.28NNAYY23 pKa = 8.84TDD25 pKa = 3.91LDD27 pKa = 3.36IYY29 pKa = 10.65TFVEE33 pKa = 4.59LEE35 pKa = 4.33CLTKK39 pKa = 10.38EE40 pKa = 4.05QYY42 pKa = 10.12TEE44 pKa = 3.63ITGDD48 pKa = 3.47NFPQEE53 pKa = 4.12TEE55 pKa = 3.99EE56 pKa = 4.56NDD58 pKa = 3.45FNEE61 pKa = 4.19QPKK64 pKa = 9.78PYY66 pKa = 10.24QPISDD71 pKa = 3.96TEE73 pKa = 3.91

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D6Z2A0|A0A1D6Z2A0_9CAUD Capsid protein OS=Staphylococcus phage vB_SauS_IMEP5 OX=1852565 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.26DD3 pKa = 2.7AFYY6 pKa = 9.84PYY8 pKa = 9.9RR9 pKa = 11.84DD10 pKa = 3.06TGYY13 pKa = 8.61TVTDD17 pKa = 3.51MNLYY21 pKa = 10.09GPNKK25 pKa = 8.75KK26 pKa = 8.6TGSNRR31 pKa = 11.84AKK33 pKa = 10.76LNWQGPHH40 pKa = 5.9KK41 pKa = 10.09RR42 pKa = 11.84YY43 pKa = 10.77KK44 pKa = 10.65LMHH47 pKa = 6.53LNEE50 pKa = 5.02FGHH53 pKa = 6.44FMRR56 pKa = 11.84NGQFLSPRR64 pKa = 11.84QTGLIEE70 pKa = 3.9NTTRR74 pKa = 11.84ASRR77 pKa = 11.84DD78 pKa = 3.31SYY80 pKa = 11.11RR81 pKa = 11.84KK82 pKa = 9.58AIKK85 pKa = 10.21EE86 pKa = 3.95EE87 pKa = 4.06LEE89 pKa = 4.01KK90 pKa = 10.79RR91 pKa = 11.84VV92 pKa = 3.31

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13426

41

1605

200.4

22.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.638 ± 0.329

0.536 ± 0.085

6.428 ± 0.412

7.612 ± 0.684

3.925 ± 0.223

6.599 ± 0.7

1.586 ± 0.129

6.905 ± 0.252

9.467 ± 0.424

7.515 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.19

6.852 ± 0.25

2.957 ± 0.154

3.724 ± 0.193

3.888 ± 0.259

5.765 ± 0.25

6.346 ± 0.259

6.257 ± 0.291

1.132 ± 0.187

4.216 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski