Rhodococcus phage Weasels2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Weaselvirus; Rhodococcus virus Weasel

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9SA09|A0A1I9SA09_9CAUD Uncharacterized protein OS=Rhodococcus phage Weasels2 OX=1897437 GN=SEA_WEASELS2_25 PE=4 SV=1
MM1 pKa = 7.83NDD3 pKa = 3.5RR4 pKa = 11.84QTKK7 pKa = 10.13LYY9 pKa = 10.21YY10 pKa = 10.6DD11 pKa = 4.2LDD13 pKa = 4.0EE14 pKa = 4.91VPPHH18 pKa = 5.72VAFSCFINGNKK29 pKa = 9.56EE30 pKa = 3.96DD31 pKa = 4.73LKK33 pKa = 11.2LSYY36 pKa = 10.72NEE38 pKa = 3.96PDD40 pKa = 3.13VYY42 pKa = 10.95EE43 pKa = 4.56YY44 pKa = 11.25LDD46 pKa = 5.47EE47 pKa = 5.86DD48 pKa = 4.31DD49 pKa = 3.57TWIAGDD55 pKa = 4.06FLAEE59 pKa = 3.99YY60 pKa = 10.94GPFLVTLDD68 pKa = 3.83NDD70 pKa = 3.91STLDD74 pKa = 3.33

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9S9Z2|A0A1I9S9Z2_9CAUD Uncharacterized protein OS=Rhodococcus phage Weasels2 OX=1897437 GN=SEA_WEASELS2_8 PE=4 SV=1
MM1 pKa = 7.55QSIKK5 pKa = 10.15RR6 pKa = 11.84QKK8 pKa = 8.91TAFTDD13 pKa = 3.68IYY15 pKa = 10.62TSNQAINRR23 pKa = 11.84KK24 pKa = 7.3EE25 pKa = 3.6HH26 pKa = 5.79TFNVFQPINCLSQRR40 pKa = 11.84RR41 pKa = 11.84FTTHH45 pKa = 6.13YY46 pKa = 9.98SRR48 pKa = 11.84CSLLHH53 pKa = 6.28CFHH56 pKa = 7.34RR57 pKa = 11.84PQIQVCSLLCRR68 pKa = 11.84CRR70 pKa = 11.84SLSFPFTRR78 pKa = 11.84FNNSEE83 pKa = 4.01TMVRR87 pKa = 3.76

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

40818

39

2472

147.4

16.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.972 ± 0.236

1.117 ± 0.102

7.056 ± 0.156

7.391 ± 0.258

3.984 ± 0.123

6.269 ± 0.229

2.07 ± 0.103

6.272 ± 0.151

6.61 ± 0.165

8.117 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.083

4.875 ± 0.135

3.802 ± 0.13

3.325 ± 0.113

4.64 ± 0.129

6.419 ± 0.149

5.968 ± 0.223

6.649 ± 0.167

1.757 ± 0.1

4.202 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski