Amino acid dipepetide frequency for Rhodococcus phage Weasels2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.924AlaAla: 4.924 ± 0.625
0.612AlaCys: 0.612 ± 0.125
4.018AlaAsp: 4.018 ± 0.299
5.096AlaGlu: 5.096 ± 0.377
3.307AlaPhe: 3.307 ± 0.314
4.851AlaGly: 4.851 ± 0.551
1.323AlaHis: 1.323 ± 0.196
4.581AlaIle: 4.581 ± 0.369
5.316AlaLys: 5.316 ± 0.328
6.174AlaLeu: 6.174 ± 0.635
1.788AlaMet: 1.788 ± 0.215
3.16AlaAsn: 3.16 ± 0.291
2.131AlaPro: 2.131 ± 0.226
2.352AlaGln: 2.352 ± 0.281
3.062AlaArg: 3.062 ± 0.293
3.699AlaSer: 3.699 ± 0.37
4.581AlaThr: 4.581 ± 0.359
4.336AlaVal: 4.336 ± 0.402
1.739AlaTrp: 1.739 ± 0.206
2.67AlaTyr: 2.67 ± 0.268
0.0AlaXaa: 0.0 ± 0.0
Cys
0.637CysAla: 0.637 ± 0.124
0.22CysCys: 0.22 ± 0.085
0.882CysAsp: 0.882 ± 0.161
0.784CysGlu: 0.784 ± 0.138
0.318CysPhe: 0.318 ± 0.086
1.004CysGly: 1.004 ± 0.164
0.171CysHis: 0.171 ± 0.067
0.49CysIle: 0.49 ± 0.101
0.808CysLys: 0.808 ± 0.171
0.857CysLeu: 0.857 ± 0.155
0.171CysMet: 0.171 ± 0.065
0.588CysAsn: 0.588 ± 0.133
0.465CysPro: 0.465 ± 0.123
0.22CysGln: 0.22 ± 0.07
0.686CysArg: 0.686 ± 0.142
0.759CysSer: 0.759 ± 0.135
0.906CysThr: 0.906 ± 0.177
0.661CysVal: 0.661 ± 0.114
0.196CysTrp: 0.196 ± 0.071
0.343CysTyr: 0.343 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.924AspAla: 4.924 ± 0.379
0.784AspCys: 0.784 ± 0.158
4.9AspAsp: 4.9 ± 0.333
5.365AspGlu: 5.365 ± 0.504
3.65AspPhe: 3.65 ± 0.276
4.704AspGly: 4.704 ± 0.343
1.421AspHis: 1.421 ± 0.181
4.924AspIle: 4.924 ± 0.345
3.969AspLys: 3.969 ± 0.288
7.105AspLeu: 7.105 ± 0.378
1.617AspMet: 1.617 ± 0.177
2.352AspAsn: 2.352 ± 0.219
2.817AspPro: 2.817 ± 0.249
2.278AspGln: 2.278 ± 0.214
2.915AspArg: 2.915 ± 0.244
4.532AspSer: 4.532 ± 0.282
3.503AspThr: 3.503 ± 0.347
4.508AspVal: 4.508 ± 0.461
1.396AspTrp: 1.396 ± 0.159
2.891AspTyr: 2.891 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
4.949GluAla: 4.949 ± 0.386
0.931GluCys: 0.931 ± 0.151
5.537GluAsp: 5.537 ± 0.482
5.929GluGlu: 5.929 ± 0.521
3.479GluPhe: 3.479 ± 0.332
3.92GluGly: 3.92 ± 0.307
1.568GluHis: 1.568 ± 0.203
5.169GluIle: 5.169 ± 0.389
4.41GluLys: 4.41 ± 0.428
7.84GluLeu: 7.84 ± 0.391
1.813GluMet: 1.813 ± 0.209
2.989GluAsn: 2.989 ± 0.31
2.695GluPro: 2.695 ± 0.279
2.45GluGln: 2.45 ± 0.24
3.111GluArg: 3.111 ± 0.316
3.699GluSer: 3.699 ± 0.237
3.601GluThr: 3.601 ± 0.348
4.998GluVal: 4.998 ± 0.42
1.151GluTrp: 1.151 ± 0.189
3.675GluTyr: 3.675 ± 0.4
0.0GluXaa: 0.0 ± 0.0
Phe
2.082PheAla: 2.082 ± 0.214
0.343PheCys: 0.343 ± 0.1
3.479PheAsp: 3.479 ± 0.326
3.111PheGlu: 3.111 ± 0.278
1.372PhePhe: 1.372 ± 0.196
2.352PheGly: 2.352 ± 0.23
0.759PheHis: 0.759 ± 0.158
2.303PheIle: 2.303 ± 0.28
2.597PheLys: 2.597 ± 0.26
3.087PheLeu: 3.087 ± 0.228
1.053PheMet: 1.053 ± 0.165
2.621PheAsn: 2.621 ± 0.201
1.225PhePro: 1.225 ± 0.162
1.323PheGln: 1.323 ± 0.192
1.641PheArg: 1.641 ± 0.191
3.185PheSer: 3.185 ± 0.276
2.621PheThr: 2.621 ± 0.22
2.205PheVal: 2.205 ± 0.24
0.71PheTrp: 0.71 ± 0.143
1.764PheTyr: 1.764 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
3.871GlyAla: 3.871 ± 0.391
0.539GlyCys: 0.539 ± 0.12
4.263GlyAsp: 4.263 ± 0.361
3.503GlyGlu: 3.503 ± 0.255
2.793GlyPhe: 2.793 ± 0.247
3.65GlyGly: 3.65 ± 0.582
1.519GlyHis: 1.519 ± 0.232
3.92GlyIle: 3.92 ± 0.44
3.822GlyLys: 3.822 ± 0.329
5.39GlyLeu: 5.39 ± 0.404
1.592GlyMet: 1.592 ± 0.236
2.964GlyAsn: 2.964 ± 0.331
2.009GlyPro: 2.009 ± 0.283
2.156GlyGln: 2.156 ± 0.281
3.013GlyArg: 3.013 ± 0.303
4.777GlySer: 4.777 ± 0.396
4.165GlyThr: 4.165 ± 0.542
3.944GlyVal: 3.944 ± 0.319
1.666GlyTrp: 1.666 ± 0.179
3.136GlyTyr: 3.136 ± 0.337
0.0GlyXaa: 0.0 ± 0.0
His
1.372HisAla: 1.372 ± 0.212
0.318HisCys: 0.318 ± 0.092
1.641HisAsp: 1.641 ± 0.208
1.298HisGlu: 1.298 ± 0.198
1.2HisPhe: 1.2 ± 0.182
1.298HisGly: 1.298 ± 0.153
0.465HisHis: 0.465 ± 0.105
1.274HisIle: 1.274 ± 0.181
1.053HisLys: 1.053 ± 0.171
1.47HisLeu: 1.47 ± 0.185
0.49HisMet: 0.49 ± 0.103
1.029HisAsn: 1.029 ± 0.15
0.686HisPro: 0.686 ± 0.147
0.637HisGln: 0.637 ± 0.126
1.2HisArg: 1.2 ± 0.173
1.323HisSer: 1.323 ± 0.19
1.274HisThr: 1.274 ± 0.146
1.274HisVal: 1.274 ± 0.189
0.343HisTrp: 0.343 ± 0.087
1.053HisTyr: 1.053 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
5.169IleAla: 5.169 ± 0.399
0.686IleCys: 0.686 ± 0.137
5.365IleAsp: 5.365 ± 0.381
5.218IleGlu: 5.218 ± 0.354
1.47IlePhe: 1.47 ± 0.173
3.822IleGly: 3.822 ± 0.393
1.274IleHis: 1.274 ± 0.234
3.405IleIle: 3.405 ± 0.317
4.557IleLys: 4.557 ± 0.288
3.969IleLeu: 3.969 ± 0.315
1.225IleMet: 1.225 ± 0.172
3.822IleAsn: 3.822 ± 0.293
2.67IlePro: 2.67 ± 0.31
2.205IleGln: 2.205 ± 0.284
3.062IleArg: 3.062 ± 0.237
4.238IleSer: 4.238 ± 0.348
3.552IleThr: 3.552 ± 0.287
3.822IleVal: 3.822 ± 0.325
0.71IleTrp: 0.71 ± 0.16
2.45IleTyr: 2.45 ± 0.262
0.0IleXaa: 0.0 ± 0.0
Lys
5.267LysAla: 5.267 ± 0.407
0.686LysCys: 0.686 ± 0.141
4.851LysAsp: 4.851 ± 0.344
5.39LysGlu: 5.39 ± 0.479
2.499LysPhe: 2.499 ± 0.244
3.209LysGly: 3.209 ± 0.275
1.249LysHis: 1.249 ± 0.196
3.993LysIle: 3.993 ± 0.406
4.214LysLys: 4.214 ± 0.34
5.022LysLeu: 5.022 ± 0.287
2.229LysMet: 2.229 ± 0.249
3.209LysAsn: 3.209 ± 0.229
2.768LysPro: 2.768 ± 0.292
2.058LysGln: 2.058 ± 0.208
2.891LysArg: 2.891 ± 0.261
3.675LysSer: 3.675 ± 0.382
4.091LysThr: 4.091 ± 0.292
4.826LysVal: 4.826 ± 0.36
1.004LysTrp: 1.004 ± 0.178
2.964LysTyr: 2.964 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
6.076LeuAla: 6.076 ± 0.387
0.833LeuCys: 0.833 ± 0.155
6.394LeuAsp: 6.394 ± 0.437
6.517LeuGlu: 6.517 ± 0.37
2.989LeuPhe: 2.989 ± 0.256
5.463LeuGly: 5.463 ± 0.341
1.617LeuHis: 1.617 ± 0.186
4.532LeuIle: 4.532 ± 0.364
5.733LeuLys: 5.733 ± 0.337
5.194LeuLeu: 5.194 ± 0.367
2.205LeuMet: 2.205 ± 0.227
4.63LeuAsn: 4.63 ± 0.411
2.964LeuPro: 2.964 ± 0.304
2.744LeuGln: 2.744 ± 0.268
3.454LeuArg: 3.454 ± 0.317
5.365LeuSer: 5.365 ± 0.324
5.047LeuThr: 5.047 ± 0.438
4.924LeuVal: 4.924 ± 0.367
1.151LeuTrp: 1.151 ± 0.147
3.332LeuTyr: 3.332 ± 0.316
0.0LeuXaa: 0.0 ± 0.0
Met
1.862MetAla: 1.862 ± 0.23
0.294MetCys: 0.294 ± 0.084
1.372MetAsp: 1.372 ± 0.194
1.249MetGlu: 1.249 ± 0.179
0.931MetPhe: 0.931 ± 0.158
1.347MetGly: 1.347 ± 0.217
0.539MetHis: 0.539 ± 0.124
1.837MetIle: 1.837 ± 0.223
2.131MetLys: 2.131 ± 0.238
2.082MetLeu: 2.082 ± 0.249
0.71MetMet: 0.71 ± 0.158
1.47MetAsn: 1.47 ± 0.192
1.151MetPro: 1.151 ± 0.143
0.735MetGln: 0.735 ± 0.119
1.249MetArg: 1.249 ± 0.142
1.911MetSer: 1.911 ± 0.202
1.837MetThr: 1.837 ± 0.186
1.249MetVal: 1.249 ± 0.156
0.343MetTrp: 0.343 ± 0.092
0.759MetTyr: 0.759 ± 0.143
0.0MetXaa: 0.0 ± 0.0
Asn
3.307AsnAla: 3.307 ± 0.412
0.735AsnCys: 0.735 ± 0.158
2.94AsnAsp: 2.94 ± 0.277
3.43AsnGlu: 3.43 ± 0.305
1.813AsnPhe: 1.813 ± 0.209
3.92AsnGly: 3.92 ± 0.311
1.347AsnHis: 1.347 ± 0.211
2.793AsnIle: 2.793 ± 0.258
3.013AsnLys: 3.013 ± 0.325
3.944AsnLeu: 3.944 ± 0.354
0.906AsnMet: 0.906 ± 0.172
2.523AsnAsn: 2.523 ± 0.292
2.45AsnPro: 2.45 ± 0.318
1.568AsnGln: 1.568 ± 0.239
2.401AsnArg: 2.401 ± 0.262
3.381AsnSer: 3.381 ± 0.363
2.474AsnThr: 2.474 ± 0.296
3.234AsnVal: 3.234 ± 0.392
0.808AsnTrp: 0.808 ± 0.126
1.764AsnTyr: 1.764 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
2.376ProAla: 2.376 ± 0.225
0.269ProCys: 0.269 ± 0.088
2.768ProAsp: 2.768 ± 0.293
4.018ProGlu: 4.018 ± 0.33
1.323ProPhe: 1.323 ± 0.191
2.597ProGly: 2.597 ± 0.291
0.49ProHis: 0.49 ± 0.112
2.205ProIle: 2.205 ± 0.272
2.401ProLys: 2.401 ± 0.289
2.548ProLeu: 2.548 ± 0.252
0.906ProMet: 0.906 ± 0.127
2.107ProAsn: 2.107 ± 0.253
1.323ProPro: 1.323 ± 0.18
1.445ProGln: 1.445 ± 0.191
1.543ProArg: 1.543 ± 0.223
1.984ProSer: 1.984 ± 0.207
2.793ProThr: 2.793 ± 0.314
3.087ProVal: 3.087 ± 0.287
0.612ProTrp: 0.612 ± 0.127
1.225ProTyr: 1.225 ± 0.204
0.0ProXaa: 0.0 ± 0.0
Gln
3.209GlnAla: 3.209 ± 0.334
0.318GlnCys: 0.318 ± 0.09
1.911GlnAsp: 1.911 ± 0.214
2.082GlnGlu: 2.082 ± 0.207
1.078GlnPhe: 1.078 ± 0.163
1.886GlnGly: 1.886 ± 0.216
0.563GlnHis: 0.563 ± 0.138
2.229GlnIle: 2.229 ± 0.296
2.254GlnLys: 2.254 ± 0.241
2.621GlnLeu: 2.621 ± 0.278
0.955GlnMet: 0.955 ± 0.148
1.421GlnAsn: 1.421 ± 0.165
1.176GlnPro: 1.176 ± 0.144
1.249GlnGln: 1.249 ± 0.194
1.911GlnArg: 1.911 ± 0.221
1.788GlnSer: 1.788 ± 0.215
1.911GlnThr: 1.911 ± 0.27
2.474GlnVal: 2.474 ± 0.254
0.71GlnTrp: 0.71 ± 0.129
1.494GlnTyr: 1.494 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
3.16ArgAla: 3.16 ± 0.283
0.441ArgCys: 0.441 ± 0.105
2.646ArgAsp: 2.646 ± 0.264
2.842ArgGlu: 2.842 ± 0.276
1.641ArgPhe: 1.641 ± 0.219
2.646ArgGly: 2.646 ± 0.255
1.029ArgHis: 1.029 ± 0.151
3.283ArgIle: 3.283 ± 0.253
4.018ArgLys: 4.018 ± 0.382
3.944ArgLeu: 3.944 ± 0.342
1.323ArgMet: 1.323 ± 0.167
2.107ArgAsn: 2.107 ± 0.217
1.886ArgPro: 1.886 ± 0.173
2.058ArgGln: 2.058 ± 0.228
2.205ArgArg: 2.205 ± 0.232
2.352ArgSer: 2.352 ± 0.259
2.695ArgThr: 2.695 ± 0.245
3.013ArgVal: 3.013 ± 0.278
0.882ArgTrp: 0.882 ± 0.172
2.229ArgTyr: 2.229 ± 0.255
0.0ArgXaa: 0.0 ± 0.0
Ser
4.067SerAla: 4.067 ± 0.324
0.735SerCys: 0.735 ± 0.132
4.361SerAsp: 4.361 ± 0.332
4.336SerGlu: 4.336 ± 0.313
2.866SerPhe: 2.866 ± 0.288
4.189SerGly: 4.189 ± 0.383
1.372SerHis: 1.372 ± 0.206
4.067SerIle: 4.067 ± 0.429
4.067SerLys: 4.067 ± 0.358
5.292SerLeu: 5.292 ± 0.368
1.568SerMet: 1.568 ± 0.194
3.087SerAsn: 3.087 ± 0.247
2.107SerPro: 2.107 ± 0.257
2.131SerGln: 2.131 ± 0.231
3.626SerArg: 3.626 ± 0.324
4.581SerSer: 4.581 ± 0.475
3.503SerThr: 3.503 ± 0.34
4.385SerVal: 4.385 ± 0.351
1.004SerTrp: 1.004 ± 0.171
2.842SerTyr: 2.842 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
4.41ThrAla: 4.41 ± 0.452
1.004ThrCys: 1.004 ± 0.175
3.65ThrAsp: 3.65 ± 0.266
3.773ThrGlu: 3.773 ± 0.335
2.499ThrPhe: 2.499 ± 0.254
3.969ThrGly: 3.969 ± 0.468
1.249ThrHis: 1.249 ± 0.148
4.091ThrIle: 4.091 ± 0.39
3.846ThrLys: 3.846 ± 0.306
4.973ThrLeu: 4.973 ± 0.352
1.127ThrMet: 1.127 ± 0.169
2.621ThrAsn: 2.621 ± 0.276
2.401ThrPro: 2.401 ± 0.219
1.592ThrGln: 1.592 ± 0.217
2.597ThrArg: 2.597 ± 0.263
4.483ThrSer: 4.483 ± 0.457
3.969ThrThr: 3.969 ± 0.448
4.459ThrVal: 4.459 ± 0.349
1.004ThrTrp: 1.004 ± 0.164
1.96ThrTyr: 1.96 ± 0.212
0.0ThrXaa: 0.0 ± 0.0
Val
4.532ValAla: 4.532 ± 0.368
0.784ValCys: 0.784 ± 0.137
5.341ValAsp: 5.341 ± 0.556
5.806ValGlu: 5.806 ± 0.413
2.474ValPhe: 2.474 ± 0.26
4.165ValGly: 4.165 ± 0.332
1.249ValHis: 1.249 ± 0.145
4.042ValIle: 4.042 ± 0.31
4.263ValLys: 4.263 ± 0.352
4.679ValLeu: 4.679 ± 0.348
1.617ValMet: 1.617 ± 0.177
2.989ValAsn: 2.989 ± 0.322
2.817ValPro: 2.817 ± 0.26
2.009ValGln: 2.009 ± 0.209
3.111ValArg: 3.111 ± 0.252
4.41ValSer: 4.41 ± 0.326
3.405ValThr: 3.405 ± 0.361
5.047ValVal: 5.047 ± 0.41
1.078ValTrp: 1.078 ± 0.158
2.67ValTyr: 2.67 ± 0.261
0.0ValXaa: 0.0 ± 0.0
Trp
0.98TrpAla: 0.98 ± 0.152
0.294TrpCys: 0.294 ± 0.097
1.029TrpAsp: 1.029 ± 0.186
1.225TrpGlu: 1.225 ± 0.146
1.004TrpPhe: 1.004 ± 0.175
0.906TrpGly: 0.906 ± 0.165
0.514TrpHis: 0.514 ± 0.12
1.225TrpIle: 1.225 ± 0.18
1.053TrpLys: 1.053 ± 0.182
1.617TrpLeu: 1.617 ± 0.236
0.612TrpMet: 0.612 ± 0.125
1.127TrpAsn: 1.127 ± 0.188
0.637TrpPro: 0.637 ± 0.133
0.563TrpGln: 0.563 ± 0.12
0.784TrpArg: 0.784 ± 0.153
1.225TrpSer: 1.225 ± 0.243
1.078TrpThr: 1.078 ± 0.153
0.98TrpVal: 0.98 ± 0.178
0.269TrpTrp: 0.269 ± 0.086
0.441TrpTyr: 0.441 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.548TyrAla: 2.548 ± 0.304
0.343TyrCys: 0.343 ± 0.096
3.209TyrAsp: 3.209 ± 0.284
2.94TyrGlu: 2.94 ± 0.281
1.127TyrPhe: 1.127 ± 0.165
2.793TyrGly: 2.793 ± 0.309
0.98TyrHis: 0.98 ± 0.175
2.352TyrIle: 2.352 ± 0.32
2.572TyrLys: 2.572 ± 0.271
3.356TyrLeu: 3.356 ± 0.306
1.127TyrMet: 1.127 ± 0.172
1.935TyrAsn: 1.935 ± 0.236
1.641TyrPro: 1.641 ± 0.207
1.47TyrGln: 1.47 ± 0.178
1.935TyrArg: 1.935 ± 0.217
2.817TyrSer: 2.817 ± 0.305
2.67TyrThr: 2.67 ± 0.246
3.062TyrVal: 3.062 ± 0.315
0.784TyrTrp: 0.784 ± 0.125
2.352TyrTyr: 2.352 ± 0.277
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 277 proteins (40818 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski