Arthrobacter phage Adaia
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G2KCU7|A0A3G2KCU7_9CAUD HNH endonuclease OS=Arthrobacter phage Adaia OX=2419945 GN=28 PE=4 SV=1
MM1 pKa = 7.51 AALAIEE7 pKa = 4.44 SGIPPSVLEE16 pKa = 4.47 RR17 pKa = 11.84 EE18 pKa = 3.87 PSEE21 pKa = 4.19 YY22 pKa = 10.79 LEE24 pKa = 4.42 TMCYY28 pKa = 9.86 LAIHH32 pKa = 7.11 RR33 pKa = 11.84 YY34 pKa = 10.2 DD35 pKa = 3.53 EE36 pKa = 4.67 TEE38 pKa = 3.9 GKK40 pKa = 10.19
Molecular weight: 4.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.226
IPC2_protein 4.596
IPC_protein 4.342
Toseland 4.228
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.101
Rodwell 4.202
Grimsley 4.151
Solomon 4.253
Lehninger 4.202
Nozaki 4.393
DTASelect 4.406
Thurlkill 4.228
EMBOSS 4.126
Sillero 4.457
Patrickios 1.977
IPC_peptide 4.266
IPC2_peptide 4.444
IPC2.peptide.svr19 4.382
Protein with the highest isoelectric point:
>tr|A0A3G2KCQ9|A0A3G2KCQ9_9CAUD Uncharacterized protein OS=Arthrobacter phage Adaia OX=2419945 GN=19 PE=4 SV=1
MM1 pKa = 8.0 AEE3 pKa = 4.34 PLIKK7 pKa = 10.6 VYY9 pKa = 10.74 GGPEE13 pKa = 3.15 LRR15 pKa = 11.84 ARR17 pKa = 11.84 MRR19 pKa = 11.84 KK20 pKa = 9.87 AGVEE24 pKa = 4.02 MQNLRR29 pKa = 11.84 DD30 pKa = 3.56 ANARR34 pKa = 11.84 VAKK37 pKa = 9.84 VAEE40 pKa = 4.35 GGIRR44 pKa = 11.84 AAAPKK49 pKa = 10.34 KK50 pKa = 9.9 SGRR53 pKa = 11.84 LAGTIRR59 pKa = 11.84 SSGTLRR65 pKa = 11.84 AAIVRR70 pKa = 11.84 AGYY73 pKa = 9.96 KK74 pKa = 7.89 ATPYY78 pKa = 10.49 AGPNNWGWPDD88 pKa = 3.1 GAAGIRR94 pKa = 11.84 GSFGGDD100 pKa = 2.82 FWAQAGARR108 pKa = 11.84 ATEE111 pKa = 4.35 SAWVAVYY118 pKa = 10.12 HH119 pKa = 6.89 SEE121 pKa = 3.82 IQKK124 pKa = 10.34 IADD127 pKa = 3.39 QVV129 pKa = 3.3
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.692
IPC_protein 10.365
Toseland 10.57
ProMoST 10.233
Dawson 10.687
Bjellqvist 10.379
Wikipedia 10.877
Rodwell 10.979
Grimsley 10.745
Solomon 10.774
Lehninger 10.745
Nozaki 10.526
DTASelect 10.379
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.716
IPC_peptide 10.774
IPC2_peptide 9.063
IPC2.peptide.svr19 8.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
4967
40
638
177.4
19.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.1 ± 0.728
0.443 ± 0.195
5.174 ± 0.484
5.798 ± 0.527
3.282 ± 0.269
8.979 ± 0.659
1.651 ± 0.296
5.355 ± 0.537
5.194 ± 0.639
8.073 ± 0.314
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.22
3.886 ± 0.328
4.57 ± 0.499
4.047 ± 0.347
4.912 ± 0.514
6.161 ± 0.288
6.906 ± 0.508
6.785 ± 0.487
1.852 ± 0.174
2.194 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here