Amino acid dipepetide frequency for Arthrobacter phage Adaia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.512AlaAla: 16.512 ± 2.297
0.604AlaCys: 0.604 ± 0.36
4.833AlaAsp: 4.833 ± 0.886
6.847AlaGlu: 6.847 ± 0.857
4.631AlaPhe: 4.631 ± 1.026
12.686AlaGly: 12.686 ± 2.116
1.611AlaHis: 1.611 ± 0.335
8.055AlaIle: 8.055 ± 2.091
6.444AlaLys: 6.444 ± 1.167
9.464AlaLeu: 9.464 ± 1.287
4.229AlaMet: 4.229 ± 0.928
3.222AlaAsn: 3.222 ± 1.036
5.437AlaPro: 5.437 ± 1.592
4.833AlaGln: 4.833 ± 0.999
8.055AlaArg: 8.055 ± 1.339
3.826AlaSer: 3.826 ± 1.097
7.652AlaThr: 7.652 ± 1.177
8.055AlaVal: 8.055 ± 2.064
1.41AlaTrp: 1.41 ± 0.436
2.618AlaTyr: 2.618 ± 0.867
0.0AlaXaa: 0.0 ± 0.0
Cys
0.201CysAla: 0.201 ± 0.19
0.201CysCys: 0.201 ± 0.257
0.0CysAsp: 0.0 ± 0.0
0.201CysGlu: 0.201 ± 0.223
0.0CysPhe: 0.0 ± 0.0
0.403CysGly: 0.403 ± 0.436
0.201CysHis: 0.201 ± 0.218
0.201CysIle: 0.201 ± 0.214
0.604CysLys: 0.604 ± 0.36
0.0CysLeu: 0.0 ± 0.0
0.201CysMet: 0.201 ± 0.205
0.0CysAsn: 0.0 ± 0.0
0.403CysPro: 0.403 ± 0.35
0.604CysGln: 0.604 ± 0.343
0.201CysArg: 0.201 ± 0.177
0.201CysSer: 0.201 ± 0.246
0.604CysThr: 0.604 ± 0.388
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.201CysTyr: 0.201 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
6.041AspAla: 6.041 ± 0.946
0.0AspCys: 0.0 ± 0.0
3.222AspAsp: 3.222 ± 0.694
1.41AspGlu: 1.41 ± 0.497
1.812AspPhe: 1.812 ± 0.62
3.423AspGly: 3.423 ± 0.775
1.41AspHis: 1.41 ± 0.624
2.416AspIle: 2.416 ± 0.7
2.215AspLys: 2.215 ± 0.69
4.631AspLeu: 4.631 ± 0.678
1.812AspMet: 1.812 ± 0.746
3.222AspAsn: 3.222 ± 0.715
2.014AspPro: 2.014 ± 0.579
1.41AspGln: 1.41 ± 0.482
1.41AspArg: 1.41 ± 0.463
3.625AspSer: 3.625 ± 0.8
3.826AspThr: 3.826 ± 0.925
5.034AspVal: 5.034 ± 1.112
1.611AspTrp: 1.611 ± 0.628
1.208AspTyr: 1.208 ± 0.508
0.0AspXaa: 0.0 ± 0.0
Glu
6.444GluAla: 6.444 ± 1.003
0.201GluCys: 0.201 ± 0.223
3.021GluAsp: 3.021 ± 0.859
1.41GluGlu: 1.41 ± 0.825
1.812GluPhe: 1.812 ± 0.45
4.027GluGly: 4.027 ± 0.661
0.604GluHis: 0.604 ± 0.58
2.416GluIle: 2.416 ± 0.516
2.819GluLys: 2.819 ± 0.8
7.249GluLeu: 7.249 ± 1.14
1.611GluMet: 1.611 ± 0.487
2.215GluAsn: 2.215 ± 0.694
3.222GluPro: 3.222 ± 0.827
2.014GluGln: 2.014 ± 0.684
3.222GluArg: 3.222 ± 0.688
3.826GluSer: 3.826 ± 0.863
3.826GluThr: 3.826 ± 1.107
4.229GluVal: 4.229 ± 1.288
1.611GluTrp: 1.611 ± 0.73
2.215GluTyr: 2.215 ± 0.662
0.0GluXaa: 0.0 ± 0.0
Phe
4.631PheAla: 4.631 ± 1.112
0.0PheCys: 0.0 ± 0.0
1.41PheAsp: 1.41 ± 0.568
2.819PheGlu: 2.819 ± 0.775
0.805PhePhe: 0.805 ± 0.435
3.625PheGly: 3.625 ± 0.87
0.201PheHis: 0.201 ± 0.205
0.805PheIle: 0.805 ± 0.448
3.222PheLys: 3.222 ± 1.017
1.611PheLeu: 1.611 ± 0.544
0.805PheMet: 0.805 ± 0.364
2.014PheAsn: 2.014 ± 0.508
1.208PhePro: 1.208 ± 0.4
0.604PheGln: 0.604 ± 0.308
1.007PheArg: 1.007 ± 0.456
1.208PheSer: 1.208 ± 0.389
2.819PheThr: 2.819 ± 0.614
2.215PheVal: 2.215 ± 0.592
1.007PheTrp: 1.007 ± 0.455
0.805PheTyr: 0.805 ± 0.431
0.0PheXaa: 0.0 ± 0.0
Gly
7.451GlyAla: 7.451 ± 1.764
0.604GlyCys: 0.604 ± 0.468
4.631GlyAsp: 4.631 ± 0.952
5.034GlyGlu: 5.034 ± 1.188
3.021GlyPhe: 3.021 ± 1.204
10.068GlyGly: 10.068 ± 1.45
2.014GlyHis: 2.014 ± 0.852
5.236GlyIle: 5.236 ± 0.904
4.631GlyLys: 4.631 ± 1.155
7.048GlyLeu: 7.048 ± 1.346
0.805GlyMet: 0.805 ± 0.397
3.826GlyAsn: 3.826 ± 0.7
1.812GlyPro: 1.812 ± 0.588
4.631GlyGln: 4.631 ± 1.009
4.631GlyArg: 4.631 ± 0.898
6.645GlySer: 6.645 ± 0.995
6.847GlyThr: 6.847 ± 1.202
6.645GlyVal: 6.645 ± 1.438
1.41GlyTrp: 1.41 ± 0.425
2.819GlyTyr: 2.819 ± 0.933
0.0GlyXaa: 0.0 ± 0.0
His
1.611HisAla: 1.611 ± 0.485
0.0HisCys: 0.0 ± 0.0
2.215HisAsp: 2.215 ± 0.89
1.208HisGlu: 1.208 ± 0.453
0.604HisPhe: 0.604 ± 0.269
1.812HisGly: 1.812 ± 0.684
0.403HisHis: 0.403 ± 0.314
0.201HisIle: 0.201 ± 0.229
1.007HisLys: 1.007 ± 0.433
1.007HisLeu: 1.007 ± 0.687
0.0HisMet: 0.0 ± 0.0
0.604HisAsn: 0.604 ± 0.426
0.604HisPro: 0.604 ± 0.364
1.007HisGln: 1.007 ± 0.503
0.403HisArg: 0.403 ± 0.284
1.208HisSer: 1.208 ± 0.44
1.41HisThr: 1.41 ± 0.62
0.604HisVal: 0.604 ± 0.322
0.201HisTrp: 0.201 ± 0.218
0.403HisTyr: 0.403 ± 0.29
0.0HisXaa: 0.0 ± 0.0
Ile
6.242IleAla: 6.242 ± 0.819
0.201IleCys: 0.201 ± 0.214
4.833IleAsp: 4.833 ± 0.976
4.229IleGlu: 4.229 ± 0.894
2.215IlePhe: 2.215 ± 0.533
4.631IleGly: 4.631 ± 1.374
1.007IleHis: 1.007 ± 0.407
3.222IleIle: 3.222 ± 1.106
4.027IleLys: 4.027 ± 0.871
2.416IleLeu: 2.416 ± 0.736
0.805IleMet: 0.805 ± 0.491
1.41IleAsn: 1.41 ± 0.687
2.618IlePro: 2.618 ± 0.514
2.215IleGln: 2.215 ± 0.58
2.618IleArg: 2.618 ± 0.805
2.014IleSer: 2.014 ± 0.648
3.625IleThr: 3.625 ± 0.921
2.819IleVal: 2.819 ± 0.483
1.007IleTrp: 1.007 ± 0.446
1.41IleTyr: 1.41 ± 0.598
0.0IleXaa: 0.0 ± 0.0
Lys
6.444LysAla: 6.444 ± 1.675
0.0LysCys: 0.0 ± 0.0
1.41LysAsp: 1.41 ± 0.658
2.819LysGlu: 2.819 ± 0.812
0.805LysPhe: 0.805 ± 0.453
3.021LysGly: 3.021 ± 1.11
0.201LysHis: 0.201 ± 0.173
3.826LysIle: 3.826 ± 0.972
2.416LysLys: 2.416 ± 0.829
5.638LysLeu: 5.638 ± 0.885
2.215LysMet: 2.215 ± 0.721
1.812LysAsn: 1.812 ± 0.687
3.021LysPro: 3.021 ± 0.533
2.014LysGln: 2.014 ± 0.646
3.423LysArg: 3.423 ± 0.819
3.826LysSer: 3.826 ± 1.237
2.618LysThr: 2.618 ± 0.617
3.222LysVal: 3.222 ± 0.915
0.805LysTrp: 0.805 ± 0.381
2.215LysTyr: 2.215 ± 0.643
0.0LysXaa: 0.0 ± 0.0
Leu
8.659LeuAla: 8.659 ± 1.221
0.604LeuCys: 0.604 ± 0.388
3.625LeuAsp: 3.625 ± 1.076
5.84LeuGlu: 5.84 ± 0.75
2.215LeuPhe: 2.215 ± 0.624
6.444LeuGly: 6.444 ± 1.636
1.812LeuHis: 1.812 ± 0.793
4.833LeuIle: 4.833 ± 1.135
4.43LeuLys: 4.43 ± 1.08
4.43LeuLeu: 4.43 ± 0.902
2.014LeuMet: 2.014 ± 0.654
2.819LeuAsn: 2.819 ± 0.668
4.229LeuPro: 4.229 ± 0.841
4.229LeuGln: 4.229 ± 0.749
4.833LeuArg: 4.833 ± 1.191
6.444LeuSer: 6.444 ± 0.643
7.652LeuThr: 7.652 ± 1.237
3.826LeuVal: 3.826 ± 0.732
1.007LeuTrp: 1.007 ± 0.349
0.805LeuTyr: 0.805 ± 0.458
0.0LeuXaa: 0.0 ± 0.0
Met
4.027MetAla: 4.027 ± 0.78
0.201MetCys: 0.201 ± 0.206
1.812MetAsp: 1.812 ± 0.791
0.604MetGlu: 0.604 ± 0.374
0.604MetPhe: 0.604 ± 0.262
2.014MetGly: 2.014 ± 0.69
0.805MetHis: 0.805 ± 0.351
0.604MetIle: 0.604 ± 0.413
0.805MetLys: 0.805 ± 0.387
2.618MetLeu: 2.618 ± 0.716
0.805MetMet: 0.805 ± 0.355
2.014MetAsn: 2.014 ± 0.484
1.208MetPro: 1.208 ± 0.495
1.007MetGln: 1.007 ± 0.548
2.215MetArg: 2.215 ± 0.738
1.611MetSer: 1.611 ± 0.728
2.215MetThr: 2.215 ± 0.568
1.208MetVal: 1.208 ± 0.569
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
5.437AsnAla: 5.437 ± 1.294
0.0AsnCys: 0.0 ± 0.0
1.208AsnAsp: 1.208 ± 0.668
2.618AsnGlu: 2.618 ± 0.921
2.215AsnPhe: 2.215 ± 0.742
3.826AsnGly: 3.826 ± 0.931
1.41AsnHis: 1.41 ± 0.516
1.812AsnIle: 1.812 ± 0.477
1.41AsnLys: 1.41 ± 0.616
3.222AsnLeu: 3.222 ± 0.777
0.403AsnMet: 0.403 ± 0.349
0.604AsnAsn: 0.604 ± 0.306
2.416AsnPro: 2.416 ± 0.657
1.611AsnGln: 1.611 ± 0.65
1.611AsnArg: 1.611 ± 0.697
2.215AsnSer: 2.215 ± 0.512
2.215AsnThr: 2.215 ± 0.773
2.416AsnVal: 2.416 ± 0.782
1.007AsnTrp: 1.007 ± 0.425
1.208AsnTyr: 1.208 ± 0.419
0.0AsnXaa: 0.0 ± 0.0
Pro
7.249ProAla: 7.249 ± 1.155
0.201ProCys: 0.201 ± 0.246
2.618ProAsp: 2.618 ± 0.797
2.416ProGlu: 2.416 ± 0.699
1.611ProPhe: 1.611 ± 0.544
3.826ProGly: 3.826 ± 0.905
0.805ProHis: 0.805 ± 0.444
3.021ProIle: 3.021 ± 1.066
2.215ProLys: 2.215 ± 0.498
3.222ProLeu: 3.222 ± 0.771
0.805ProMet: 0.805 ± 0.533
1.812ProAsn: 1.812 ± 0.611
1.41ProPro: 1.41 ± 0.486
2.014ProGln: 2.014 ± 0.606
1.611ProArg: 1.611 ± 0.474
2.014ProSer: 2.014 ± 0.614
3.222ProThr: 3.222 ± 1.196
3.222ProVal: 3.222 ± 0.942
1.007ProTrp: 1.007 ± 0.386
1.41ProTyr: 1.41 ± 0.516
0.0ProXaa: 0.0 ± 0.0
Gln
4.631GlnAla: 4.631 ± 1.116
0.403GlnCys: 0.403 ± 0.248
1.41GlnAsp: 1.41 ± 0.628
1.812GlnGlu: 1.812 ± 0.574
1.812GlnPhe: 1.812 ± 0.617
3.826GlnGly: 3.826 ± 1.03
0.805GlnHis: 0.805 ± 0.422
3.021GlnIle: 3.021 ± 0.791
1.007GlnLys: 1.007 ± 0.524
3.826GlnLeu: 3.826 ± 0.658
1.007GlnMet: 1.007 ± 0.445
1.41GlnAsn: 1.41 ± 0.566
2.819GlnPro: 2.819 ± 0.622
1.812GlnGln: 1.812 ± 0.737
1.812GlnArg: 1.812 ± 0.586
2.014GlnSer: 2.014 ± 0.769
2.819GlnThr: 2.819 ± 0.557
3.021GlnVal: 3.021 ± 0.941
0.604GlnTrp: 0.604 ± 0.415
0.604GlnTyr: 0.604 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
6.645ArgAla: 6.645 ± 1.585
0.403ArgCys: 0.403 ± 0.35
2.416ArgAsp: 2.416 ± 0.765
3.423ArgGlu: 3.423 ± 0.68
1.208ArgPhe: 1.208 ± 0.571
3.021ArgGly: 3.021 ± 0.759
0.403ArgHis: 0.403 ± 0.288
2.618ArgIle: 2.618 ± 0.738
2.618ArgLys: 2.618 ± 0.633
4.027ArgLeu: 4.027 ± 1.03
1.812ArgMet: 1.812 ± 0.622
3.222ArgAsn: 3.222 ± 0.887
1.812ArgPro: 1.812 ± 0.537
1.611ArgGln: 1.611 ± 0.844
2.215ArgArg: 2.215 ± 0.603
2.819ArgSer: 2.819 ± 0.82
2.819ArgThr: 2.819 ± 0.839
4.027ArgVal: 4.027 ± 1.093
1.007ArgTrp: 1.007 ± 0.34
1.007ArgTyr: 1.007 ± 0.491
0.0ArgXaa: 0.0 ± 0.0
Ser
7.048SerAla: 7.048 ± 1.148
0.403SerCys: 0.403 ± 0.409
2.819SerAsp: 2.819 ± 0.793
4.43SerGlu: 4.43 ± 1.098
2.416SerPhe: 2.416 ± 0.664
6.645SerGly: 6.645 ± 1.373
0.604SerHis: 0.604 ± 0.259
3.423SerIle: 3.423 ± 0.836
2.416SerLys: 2.416 ± 0.681
4.631SerLeu: 4.631 ± 0.852
2.416SerMet: 2.416 ± 0.803
1.208SerAsn: 1.208 ± 0.49
2.819SerPro: 2.819 ± 0.602
1.812SerGln: 1.812 ± 0.545
3.625SerArg: 3.625 ± 0.998
5.034SerSer: 5.034 ± 1.05
3.423SerThr: 3.423 ± 0.899
3.826SerVal: 3.826 ± 0.904
1.812SerTrp: 1.812 ± 0.562
0.805SerTyr: 0.805 ± 0.392
0.0SerXaa: 0.0 ± 0.0
Thr
8.659ThrAla: 8.659 ± 1.147
0.201ThrCys: 0.201 ± 0.19
2.819ThrAsp: 2.819 ± 0.845
4.229ThrGlu: 4.229 ± 0.696
1.41ThrPhe: 1.41 ± 0.622
7.451ThrGly: 7.451 ± 1.356
0.604ThrHis: 0.604 ± 0.287
3.625ThrIle: 3.625 ± 1.069
4.027ThrLys: 4.027 ± 1.18
5.236ThrLeu: 5.236 ± 0.748
2.215ThrMet: 2.215 ± 0.454
2.819ThrAsn: 2.819 ± 0.944
4.229ThrPro: 4.229 ± 0.897
1.812ThrGln: 1.812 ± 0.741
2.618ThrArg: 2.618 ± 0.815
3.423ThrSer: 3.423 ± 0.789
4.027ThrThr: 4.027 ± 0.944
8.055ThrVal: 8.055 ± 1.213
0.805ThrTrp: 0.805 ± 0.576
0.805ThrTyr: 0.805 ± 0.366
0.0ThrXaa: 0.0 ± 0.0
Val
7.048ValAla: 7.048 ± 0.95
0.0ValCys: 0.0 ± 0.0
3.423ValAsp: 3.423 ± 0.744
4.631ValGlu: 4.631 ± 0.654
2.618ValPhe: 2.618 ± 0.85
4.631ValGly: 4.631 ± 1.0
1.007ValHis: 1.007 ± 0.425
2.618ValIle: 2.618 ± 1.063
4.229ValLys: 4.229 ± 1.181
6.041ValLeu: 6.041 ± 1.032
1.208ValMet: 1.208 ± 0.473
3.021ValAsn: 3.021 ± 0.929
2.618ValPro: 2.618 ± 0.546
3.423ValGln: 3.423 ± 1.236
2.618ValArg: 2.618 ± 0.575
5.84ValSer: 5.84 ± 1.08
6.041ValThr: 6.041 ± 1.747
3.826ValVal: 3.826 ± 1.006
1.812ValTrp: 1.812 ± 0.676
1.208ValTyr: 1.208 ± 0.549
0.0ValXaa: 0.0 ± 0.0
Trp
2.416TrpAla: 2.416 ± 0.543
0.201TrpCys: 0.201 ± 0.218
2.215TrpAsp: 2.215 ± 0.675
1.208TrpGlu: 1.208 ± 0.402
0.403TrpPhe: 0.403 ± 0.37
1.007TrpGly: 1.007 ± 0.394
0.201TrpHis: 0.201 ± 0.211
1.007TrpIle: 1.007 ± 0.405
0.604TrpLys: 0.604 ± 0.312
2.618TrpLeu: 2.618 ± 0.951
0.201TrpMet: 0.201 ± 0.194
0.403TrpAsn: 0.403 ± 0.228
1.208TrpPro: 1.208 ± 0.452
1.208TrpGln: 1.208 ± 0.565
0.201TrpArg: 0.201 ± 0.183
1.208TrpSer: 1.208 ± 0.521
0.805TrpThr: 0.805 ± 0.389
1.007TrpVal: 1.007 ± 0.362
0.201TrpTrp: 0.201 ± 0.194
0.201TrpTyr: 0.201 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.625TyrAla: 3.625 ± 0.837
0.0TyrCys: 0.0 ± 0.0
1.812TyrAsp: 1.812 ± 0.574
0.805TyrGlu: 0.805 ± 0.347
0.604TyrPhe: 0.604 ± 0.274
3.423TyrGly: 3.423 ± 0.862
0.403TyrHis: 0.403 ± 0.267
0.604TyrIle: 0.604 ± 0.329
0.805TyrLys: 0.805 ± 0.399
1.812TyrLeu: 1.812 ± 0.578
0.805TyrMet: 0.805 ± 0.383
1.208TyrAsn: 1.208 ± 0.319
0.604TyrPro: 0.604 ± 0.354
0.604TyrGln: 0.604 ± 0.317
0.805TyrArg: 0.805 ± 0.438
2.618TyrSer: 2.618 ± 0.866
0.604TyrThr: 0.604 ± 0.427
0.604TyrVal: 0.604 ± 0.318
0.201TyrTrp: 0.201 ± 0.174
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 28 proteins (4967 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski