Lasiodiplodia theobromae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Botryosphaeriales;

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12895 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N5D1I9|A0A5N5D1I9_9PEZI 6 7-dimethyl-8-ribityllumazine synthase OS=Lasiodiplodia theobromae OX=45133 GN=DBV05_g9781 PE=3 SV=1
MM1 pKa = 7.44AVVTILSGAASGIIASAVEE20 pKa = 4.3SVIEE24 pKa = 4.42LIVDD28 pKa = 3.98LTEE31 pKa = 3.88WDD33 pKa = 3.97DD34 pKa = 3.37VRR36 pKa = 11.84KK37 pKa = 10.18AFTRR41 pKa = 11.84DD42 pKa = 2.91TVDD45 pKa = 3.52AMWGNRR51 pKa = 11.84THH53 pKa = 6.84NYY55 pKa = 9.45DD56 pKa = 4.34AAICYY61 pKa = 10.06NLDD64 pKa = 3.26YY65 pKa = 11.31DD66 pKa = 3.89VADD69 pKa = 3.4WDD71 pKa = 4.52RR72 pKa = 11.84IYY74 pKa = 11.25EE75 pKa = 4.08KK76 pKa = 10.16TAVKK80 pKa = 10.68LEE82 pKa = 4.21LGLLHH87 pKa = 6.96TDD89 pKa = 3.47YY90 pKa = 11.73DD91 pKa = 4.23CFFMNGEE98 pKa = 3.83NDD100 pKa = 3.13FWALEE105 pKa = 4.29DD106 pKa = 3.78GGGINLAATSRR117 pKa = 11.84QDD119 pKa = 3.23LCSWDD124 pKa = 4.07EE125 pKa = 3.87NDD127 pKa = 6.12DD128 pKa = 4.35LHH130 pKa = 6.87LCCDD134 pKa = 3.48

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N5D1U1|A0A5N5D1U1_9PEZI Flavin_Reduct domain-containing protein OS=Lasiodiplodia theobromae OX=45133 GN=DBV05_g9688 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 4.93EE3 pKa = 4.45EE4 pKa = 4.58RR5 pKa = 11.84PPTPIIIAGTTVSVTMRR22 pKa = 11.84VRR24 pKa = 11.84PLARR28 pKa = 11.84DD29 pKa = 3.32GSTAQSPVWSSIVEE43 pKa = 3.86RR44 pKa = 11.84RR45 pKa = 11.84HH46 pKa = 6.06ANHH49 pKa = 6.38SGIPASEE56 pKa = 4.32SFGRR60 pKa = 11.84AYY62 pKa = 10.96SAMEE66 pKa = 3.79ARR68 pKa = 11.84MHH70 pKa = 4.62QQQRR74 pKa = 11.84EE75 pKa = 4.01RR76 pKa = 11.84MQQRR80 pKa = 11.84RR81 pKa = 11.84PSSTVPQLVASPQNGRR97 pKa = 11.84RR98 pKa = 11.84PPGIEE103 pKa = 3.89EE104 pKa = 4.08PMSTSVRR111 pKa = 11.84TKK113 pKa = 10.34ASLRR117 pKa = 11.84RR118 pKa = 11.84LAHH121 pKa = 6.3KK122 pKa = 10.15LKK124 pKa = 10.83KK125 pKa = 10.56LFFFKK130 pKa = 10.59KK131 pKa = 9.62PAPTPEE137 pKa = 3.95QAQCPCFHH145 pKa = 6.8YY146 pKa = 10.47PPEE149 pKa = 3.91GWRR152 pKa = 11.84FCRR155 pKa = 11.84YY156 pKa = 9.6DD157 pKa = 3.21EE158 pKa = 4.76HH159 pKa = 7.84VAEE162 pKa = 5.81GIRR165 pKa = 11.84GIAARR170 pKa = 11.84EE171 pKa = 3.64IALGG175 pKa = 3.59

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12784

111

12895

6232502

33

15848

483.3

53.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.711 ± 0.021

1.17 ± 0.009

5.84 ± 0.021

6.287 ± 0.027

3.649 ± 0.013

7.23 ± 0.025

2.347 ± 0.01

4.424 ± 0.016

4.639 ± 0.023

8.619 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.01

3.513 ± 0.012

6.176 ± 0.03

3.88 ± 0.016

6.087 ± 0.024

7.892 ± 0.025

5.927 ± 0.034

6.274 ± 0.017

1.51 ± 0.009

2.719 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski