Mycobacterium phage Xavia 
Average proteome isoelectric point is 6.04 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A2U8UHZ7|A0A2U8UHZ7_9CAUD Uncharacterized protein OS=Mycobacterium phage Xavia OX=2178923 GN=33 PE=4 SV=1MM1 pKa = 7.97  RR2 pKa = 11.84  YY3 pKa = 9.54  GVAFQTAYY11 pKa = 11.12  SDD13 pKa = 3.6  FGGTTEE19 pKa = 4.1  VIEE22 pKa = 4.64  FEE24 pKa = 4.43  SEE26 pKa = 3.59  QAAQAFIDD34 pKa = 3.92  AVQPGVGVVLSLVRR48 pKa = 11.84  FDD50 pKa = 4.05  YY51 pKa = 10.56  STFEE55 pKa = 4.37  TIGG58 pKa = 3.19  
 6.32 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.815 
IPC2_protein 3.961 
IPC_protein 3.732 
Toseland    3.579 
ProMoST     3.91 
Dawson      3.719 
Bjellqvist  3.897 
Wikipedia   3.656 
Rodwell     3.592 
Grimsley    3.516 
Solomon     3.668 
Lehninger   3.617 
Nozaki      3.884 
DTASelect   3.961 
Thurlkill   3.656 
EMBOSS      3.668 
Sillero     3.859 
Patrickios  1.863 
IPC_peptide 3.668 
IPC2_peptide  3.821 
IPC2.peptide.svr19  3.79 
 Protein with the highest isoelectric point: 
>tr|A0A2U8UHJ1|A0A2U8UHJ1_9CAUD Uncharacterized protein OS=Mycobacterium phage Xavia OX=2178923 GN=63 PE=4 SV=1MM1 pKa = 7.6  KK2 pKa = 10.31  LFKK5 pKa = 10.34  RR6 pKa = 11.84  QPTAIEE12 pKa = 3.81  LHH14 pKa = 6.15  HH15 pKa = 6.57  RR16 pKa = 11.84  AILEE20 pKa = 3.78  WRR22 pKa = 11.84  RR23 pKa = 11.84  NPTISTYY30 pKa = 11.23  VMVDD34 pKa = 2.71  GRR36 pKa = 11.84  EE37 pKa = 3.9  VRR39 pKa = 11.84  RR40 pKa = 11.84  RR41 pKa = 11.84  AVDD44 pKa = 3.21  PLTRR48 pKa = 11.84  LGSLLDD54 pKa = 3.64  RR55 pKa = 11.84  VMDD58 pKa = 5.12  TIADD62 pKa = 3.74  VCGIPAPRR70 pKa = 11.84  LGTVPGAPWLIADD83 pKa = 4.17  EE84 pKa = 4.91  GGARR88 pKa = 3.72  
 9.87 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.322 
IPC2_protein 9.224 
IPC_protein 10.014 
Toseland    10.365 
ProMoST     10.716 
Dawson      10.467 
Bjellqvist  10.218 
Wikipedia   10.701 
Rodwell     10.496 
Grimsley    10.526 
Solomon     10.628 
Lehninger   10.613 
Nozaki      10.379 
DTASelect   10.204 
Thurlkill   10.379 
EMBOSS      10.774 
Sillero     10.409 
Patrickios  10.394 
IPC_peptide 10.643 
IPC2_peptide  9.37 
IPC2.peptide.svr19  8.721 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        71 
0
71 
16010
44
1256
225.5
24.59
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        11.599 ± 0.44
1.031 ± 0.139
6.658 ± 0.178
6.246 ± 0.321
2.979 ± 0.16
8.745 ± 0.498
2.13 ± 0.164
4.241 ± 0.154
3.129 ± 0.178
8.089 ± 0.23
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.936 ± 0.109
2.967 ± 0.206
6.046 ± 0.18
3.641 ± 0.218
7.258 ± 0.397
5.422 ± 0.228
6.309 ± 0.31
7.146 ± 0.31
2.024 ± 0.127
2.405 ± 0.119
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here