Mycobacterium phage Xavia
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8UHZ7|A0A2U8UHZ7_9CAUD Uncharacterized protein OS=Mycobacterium phage Xavia OX=2178923 GN=33 PE=4 SV=1
MM1 pKa = 7.97 RR2 pKa = 11.84 YY3 pKa = 9.54 GVAFQTAYY11 pKa = 11.12 SDD13 pKa = 3.6 FGGTTEE19 pKa = 4.1 VIEE22 pKa = 4.64 FEE24 pKa = 4.43 SEE26 pKa = 3.59 QAAQAFIDD34 pKa = 3.92 AVQPGVGVVLSLVRR48 pKa = 11.84 FDD50 pKa = 4.05 YY51 pKa = 10.56 STFEE55 pKa = 4.37 TIGG58 pKa = 3.19
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 3.961
IPC_protein 3.732
Toseland 3.579
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.516
Solomon 3.668
Lehninger 3.617
Nozaki 3.884
DTASelect 3.961
Thurlkill 3.656
EMBOSS 3.668
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.668
IPC2_peptide 3.821
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A2U8UHJ1|A0A2U8UHJ1_9CAUD Uncharacterized protein OS=Mycobacterium phage Xavia OX=2178923 GN=63 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.31 LFKK5 pKa = 10.34 RR6 pKa = 11.84 QPTAIEE12 pKa = 3.81 LHH14 pKa = 6.15 HH15 pKa = 6.57 RR16 pKa = 11.84 AILEE20 pKa = 3.78 WRR22 pKa = 11.84 RR23 pKa = 11.84 NPTISTYY30 pKa = 11.23 VMVDD34 pKa = 2.71 GRR36 pKa = 11.84 EE37 pKa = 3.9 VRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 AVDD44 pKa = 3.21 PLTRR48 pKa = 11.84 LGSLLDD54 pKa = 3.64 RR55 pKa = 11.84 VMDD58 pKa = 5.12 TIADD62 pKa = 3.74 VCGIPAPRR70 pKa = 11.84 LGTVPGAPWLIADD83 pKa = 4.17 EE84 pKa = 4.91 GGARR88 pKa = 3.72
Molecular weight: 9.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.224
IPC_protein 10.014
Toseland 10.365
ProMoST 10.716
Dawson 10.467
Bjellqvist 10.218
Wikipedia 10.701
Rodwell 10.496
Grimsley 10.526
Solomon 10.628
Lehninger 10.613
Nozaki 10.379
DTASelect 10.204
Thurlkill 10.379
EMBOSS 10.774
Sillero 10.409
Patrickios 10.394
IPC_peptide 10.643
IPC2_peptide 9.37
IPC2.peptide.svr19 8.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
16010
44
1256
225.5
24.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.599 ± 0.44
1.031 ± 0.139
6.658 ± 0.178
6.246 ± 0.321
2.979 ± 0.16
8.745 ± 0.498
2.13 ± 0.164
4.241 ± 0.154
3.129 ± 0.178
8.089 ± 0.23
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.936 ± 0.109
2.967 ± 0.206
6.046 ± 0.18
3.641 ± 0.218
7.258 ± 0.397
5.422 ± 0.228
6.309 ± 0.31
7.146 ± 0.31
2.024 ± 0.127
2.405 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here