Sulfitobacter sp. SK012

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter; unclassified Sulfitobacter

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A345QRS9|A0A345QRS9_9RHOB Transport permease protein OS=Sulfitobacter sp. SK012 OX=1389005 GN=C1J03_19635 PE=3 SV=1
MM1 pKa = 7.39CTMCFQLPSAPDD13 pKa = 3.38GCAFEE18 pKa = 5.02VSQPVFARR26 pKa = 11.84LRR28 pKa = 11.84EE29 pKa = 4.34GNDD32 pKa = 3.03AADD35 pKa = 4.39GVNTAYY41 pKa = 9.83TMSVGDD47 pKa = 4.43SFSGSIDD54 pKa = 3.78FNGDD58 pKa = 3.44DD59 pKa = 4.2DD60 pKa = 4.75WIAINLVSGRR70 pKa = 11.84NYY72 pKa = 10.07TISLDD77 pKa = 3.65GVTLNDD83 pKa = 3.51TYY85 pKa = 11.65LRR87 pKa = 11.84LYY89 pKa = 10.69DD90 pKa = 4.11DD91 pKa = 4.68AGSLVDD97 pKa = 5.28YY98 pKa = 11.22NDD100 pKa = 4.16DD101 pKa = 3.66ANGRR105 pKa = 11.84LDD107 pKa = 3.59SEE109 pKa = 4.52LSFRR113 pKa = 11.84LAYY116 pKa = 9.71PSGTYY121 pKa = 10.13YY122 pKa = 10.14ISAEE126 pKa = 4.12AYY128 pKa = 9.16SSFRR132 pKa = 11.84TGSYY136 pKa = 9.66EE137 pKa = 3.91LSVTGGSTPPQPPTGGAASLDD158 pKa = 3.57TLANFLTEE166 pKa = 4.56GYY168 pKa = 8.74WGGRR172 pKa = 11.84EE173 pKa = 3.64RR174 pKa = 11.84SFSTVSDD181 pKa = 4.01NIITVDD187 pKa = 3.02IGGLTSSGRR196 pKa = 11.84EE197 pKa = 3.63LARR200 pKa = 11.84WAFEE204 pKa = 3.36AWEE207 pKa = 4.22RR208 pKa = 11.84VADD211 pKa = 3.44IDD213 pKa = 4.16FRR215 pKa = 11.84EE216 pKa = 4.05VSFNADD222 pKa = 2.8IEE224 pKa = 4.42FDD226 pKa = 4.07DD227 pKa = 5.31FDD229 pKa = 6.44DD230 pKa = 4.38GAYY233 pKa = 10.59ANSQTSGGRR242 pKa = 11.84ITSSFVNVSTDD253 pKa = 2.71WLRR256 pKa = 11.84SNGTSIDD263 pKa = 3.63SYY265 pKa = 11.28SFQTYY270 pKa = 6.56VHH272 pKa = 7.14EE273 pKa = 5.04IGHH276 pKa = 6.48ALGLGHH282 pKa = 6.76QGDD285 pKa = 4.57YY286 pKa = 11.16NSNATYY292 pKa = 10.66GFDD295 pKa = 3.35EE296 pKa = 4.39NFRR299 pKa = 11.84NDD301 pKa = 2.49SWAQSVMSYY310 pKa = 10.72FDD312 pKa = 3.23QDD314 pKa = 3.41EE315 pKa = 4.37NTFTSASFARR325 pKa = 11.84LSGPMIADD333 pKa = 3.68ILAIQNLYY341 pKa = 9.91GAADD345 pKa = 4.0AGSATAGNTVYY356 pKa = 10.9GQGTTLDD363 pKa = 4.33GYY365 pKa = 10.63MGTLFDD371 pKa = 4.66ALASGRR377 pKa = 11.84TNSDD381 pKa = 3.23YY382 pKa = 10.87TGEE385 pKa = 4.78PIAFAISDD393 pKa = 3.45AGGIDD398 pKa = 4.49RR399 pKa = 11.84INLTYY404 pKa = 11.05LNGDD408 pKa = 3.29QYY410 pKa = 12.12VDD412 pKa = 3.67LRR414 pKa = 11.84QQAFSNIAGLRR425 pKa = 11.84EE426 pKa = 3.92NMSIARR432 pKa = 11.84GTTIEE437 pKa = 4.31NLNLGNGNDD446 pKa = 3.55TVYY449 pKa = 11.25GNDD452 pKa = 3.11ASNILRR458 pKa = 11.84SGGGSDD464 pKa = 5.56LLFSQNGSDD473 pKa = 3.3RR474 pKa = 11.84VFSGVGNDD482 pKa = 3.45TAYY485 pKa = 10.64GGAGNDD491 pKa = 3.67VLNGEE496 pKa = 4.55AGFDD500 pKa = 3.94QMWGGNGNDD509 pKa = 4.0YY510 pKa = 9.92LTGGAGNDD518 pKa = 3.48VLGGGGGNDD527 pKa = 3.71TLDD530 pKa = 3.65GGIGADD536 pKa = 3.27SLYY539 pKa = 11.07AGGGDD544 pKa = 3.98DD545 pKa = 4.96RR546 pKa = 11.84LLGRR550 pKa = 11.84DD551 pKa = 3.7GNDD554 pKa = 3.59NLWTSYY560 pKa = 11.77GNDD563 pKa = 3.28TVSGGNGNDD572 pKa = 3.49EE573 pKa = 4.12IGGGFGNDD581 pKa = 2.84NLYY584 pKa = 11.05AGTGNDD590 pKa = 3.28LVYY593 pKa = 10.91GGFGNDD599 pKa = 3.14VIYY602 pKa = 10.72GGTGRR607 pKa = 11.84DD608 pKa = 3.17QIFAFNQNDD617 pKa = 3.58FVDD620 pKa = 3.85AGEE623 pKa = 4.76GNDD626 pKa = 3.08IVYY629 pKa = 10.5LGRR632 pKa = 11.84GNDD635 pKa = 3.42TGRR638 pKa = 11.84GGQGDD643 pKa = 3.95DD644 pKa = 4.4AIYY647 pKa = 9.97GASGNDD653 pKa = 3.33IIAGGGGNDD662 pKa = 3.64TLTGGTGNDD671 pKa = 3.16TFIFTGGDD679 pKa = 3.19DD680 pKa = 4.75RR681 pKa = 11.84IADD684 pKa = 4.42FNASSALEE692 pKa = 4.44RR693 pKa = 11.84IDD695 pKa = 3.65FSGYY699 pKa = 10.98APISSFADD707 pKa = 3.19LRR709 pKa = 11.84SNHH712 pKa = 6.33LSSQGSNVLVNGTGGDD728 pKa = 3.4SLLILGVNLNEE739 pKa = 4.33LDD741 pKa = 3.77AGDD744 pKa = 4.09FVFF747 pKa = 5.86

Molecular weight:
78.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A345QIY8|A0A345QIY8_9RHOB Multidrug ABC transporter ATP-binding protein OS=Sulfitobacter sp. SK012 OX=1389005 GN=C1J03_04025 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84SKK41 pKa = 11.13LSAA44 pKa = 3.7

Molecular weight:
5.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4800

0

4800

1475871

34

4097

307.5

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.556 ± 0.049

0.916 ± 0.013

6.135 ± 0.036

5.572 ± 0.036

3.934 ± 0.025

8.518 ± 0.037

2.086 ± 0.018

5.555 ± 0.025

3.521 ± 0.031

9.866 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.017

2.988 ± 0.021

4.857 ± 0.026

3.384 ± 0.019

5.992 ± 0.033

5.613 ± 0.024

5.664 ± 0.028

7.219 ± 0.03

1.416 ± 0.016

2.305 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski