Sulfitobacter sp. SK012
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4800 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345QRS9|A0A345QRS9_9RHOB Transport permease protein OS=Sulfitobacter sp. SK012 OX=1389005 GN=C1J03_19635 PE=3 SV=1
MM1 pKa = 7.39 CTMCFQLPSAPDD13 pKa = 3.38 GCAFEE18 pKa = 5.02 VSQPVFARR26 pKa = 11.84 LRR28 pKa = 11.84 EE29 pKa = 4.34 GNDD32 pKa = 3.03 AADD35 pKa = 4.39 GVNTAYY41 pKa = 9.83 TMSVGDD47 pKa = 4.43 SFSGSIDD54 pKa = 3.78 FNGDD58 pKa = 3.44 DD59 pKa = 4.2 DD60 pKa = 4.75 WIAINLVSGRR70 pKa = 11.84 NYY72 pKa = 10.07 TISLDD77 pKa = 3.65 GVTLNDD83 pKa = 3.51 TYY85 pKa = 11.65 LRR87 pKa = 11.84 LYY89 pKa = 10.69 DD90 pKa = 4.11 DD91 pKa = 4.68 AGSLVDD97 pKa = 5.28 YY98 pKa = 11.22 NDD100 pKa = 4.16 DD101 pKa = 3.66 ANGRR105 pKa = 11.84 LDD107 pKa = 3.59 SEE109 pKa = 4.52 LSFRR113 pKa = 11.84 LAYY116 pKa = 9.71 PSGTYY121 pKa = 10.13 YY122 pKa = 10.14 ISAEE126 pKa = 4.12 AYY128 pKa = 9.16 SSFRR132 pKa = 11.84 TGSYY136 pKa = 9.66 EE137 pKa = 3.91 LSVTGGSTPPQPPTGGAASLDD158 pKa = 3.57 TLANFLTEE166 pKa = 4.56 GYY168 pKa = 8.74 WGGRR172 pKa = 11.84 EE173 pKa = 3.64 RR174 pKa = 11.84 SFSTVSDD181 pKa = 4.01 NIITVDD187 pKa = 3.02 IGGLTSSGRR196 pKa = 11.84 EE197 pKa = 3.63 LARR200 pKa = 11.84 WAFEE204 pKa = 3.36 AWEE207 pKa = 4.22 RR208 pKa = 11.84 VADD211 pKa = 3.44 IDD213 pKa = 4.16 FRR215 pKa = 11.84 EE216 pKa = 4.05 VSFNADD222 pKa = 2.8 IEE224 pKa = 4.42 FDD226 pKa = 4.07 DD227 pKa = 5.31 FDD229 pKa = 6.44 DD230 pKa = 4.38 GAYY233 pKa = 10.59 ANSQTSGGRR242 pKa = 11.84 ITSSFVNVSTDD253 pKa = 2.71 WLRR256 pKa = 11.84 SNGTSIDD263 pKa = 3.63 SYY265 pKa = 11.28 SFQTYY270 pKa = 6.56 VHH272 pKa = 7.14 EE273 pKa = 5.04 IGHH276 pKa = 6.48 ALGLGHH282 pKa = 6.76 QGDD285 pKa = 4.57 YY286 pKa = 11.16 NSNATYY292 pKa = 10.66 GFDD295 pKa = 3.35 EE296 pKa = 4.39 NFRR299 pKa = 11.84 NDD301 pKa = 2.49 SWAQSVMSYY310 pKa = 10.72 FDD312 pKa = 3.23 QDD314 pKa = 3.41 EE315 pKa = 4.37 NTFTSASFARR325 pKa = 11.84 LSGPMIADD333 pKa = 3.68 ILAIQNLYY341 pKa = 9.91 GAADD345 pKa = 4.0 AGSATAGNTVYY356 pKa = 10.9 GQGTTLDD363 pKa = 4.33 GYY365 pKa = 10.63 MGTLFDD371 pKa = 4.66 ALASGRR377 pKa = 11.84 TNSDD381 pKa = 3.23 YY382 pKa = 10.87 TGEE385 pKa = 4.78 PIAFAISDD393 pKa = 3.45 AGGIDD398 pKa = 4.49 RR399 pKa = 11.84 INLTYY404 pKa = 11.05 LNGDD408 pKa = 3.29 QYY410 pKa = 12.12 VDD412 pKa = 3.67 LRR414 pKa = 11.84 QQAFSNIAGLRR425 pKa = 11.84 EE426 pKa = 3.92 NMSIARR432 pKa = 11.84 GTTIEE437 pKa = 4.31 NLNLGNGNDD446 pKa = 3.55 TVYY449 pKa = 11.25 GNDD452 pKa = 3.11 ASNILRR458 pKa = 11.84 SGGGSDD464 pKa = 5.56 LLFSQNGSDD473 pKa = 3.3 RR474 pKa = 11.84 VFSGVGNDD482 pKa = 3.45 TAYY485 pKa = 10.64 GGAGNDD491 pKa = 3.67 VLNGEE496 pKa = 4.55 AGFDD500 pKa = 3.94 QMWGGNGNDD509 pKa = 4.0 YY510 pKa = 9.92 LTGGAGNDD518 pKa = 3.48 VLGGGGGNDD527 pKa = 3.71 TLDD530 pKa = 3.65 GGIGADD536 pKa = 3.27 SLYY539 pKa = 11.07 AGGGDD544 pKa = 3.98 DD545 pKa = 4.96 RR546 pKa = 11.84 LLGRR550 pKa = 11.84 DD551 pKa = 3.7 GNDD554 pKa = 3.59 NLWTSYY560 pKa = 11.77 GNDD563 pKa = 3.28 TVSGGNGNDD572 pKa = 3.49 EE573 pKa = 4.12 IGGGFGNDD581 pKa = 2.84 NLYY584 pKa = 11.05 AGTGNDD590 pKa = 3.28 LVYY593 pKa = 10.91 GGFGNDD599 pKa = 3.14 VIYY602 pKa = 10.72 GGTGRR607 pKa = 11.84 DD608 pKa = 3.17 QIFAFNQNDD617 pKa = 3.58 FVDD620 pKa = 3.85 AGEE623 pKa = 4.76 GNDD626 pKa = 3.08 IVYY629 pKa = 10.5 LGRR632 pKa = 11.84 GNDD635 pKa = 3.42 TGRR638 pKa = 11.84 GGQGDD643 pKa = 3.95 DD644 pKa = 4.4 AIYY647 pKa = 9.97 GASGNDD653 pKa = 3.33 IIAGGGGNDD662 pKa = 3.64 TLTGGTGNDD671 pKa = 3.16 TFIFTGGDD679 pKa = 3.19 DD680 pKa = 4.75 RR681 pKa = 11.84 IADD684 pKa = 4.42 FNASSALEE692 pKa = 4.44 RR693 pKa = 11.84 IDD695 pKa = 3.65 FSGYY699 pKa = 10.98 APISSFADD707 pKa = 3.19 LRR709 pKa = 11.84 SNHH712 pKa = 6.33 LSSQGSNVLVNGTGGDD728 pKa = 3.4 SLLILGVNLNEE739 pKa = 4.33 LDD741 pKa = 3.77 AGDD744 pKa = 4.09 FVFF747 pKa = 5.86
Molecular weight: 78.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.63
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.35
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.126
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.821
Patrickios 0.96
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A345QIY8|A0A345QIY8_9RHOB Multidrug ABC transporter ATP-binding protein OS=Sulfitobacter sp. SK012 OX=1389005 GN=C1J03_04025 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 SKK41 pKa = 11.13 LSAA44 pKa = 3.7
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4800
0
4800
1475871
34
4097
307.5
33.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.556 ± 0.049
0.916 ± 0.013
6.135 ± 0.036
5.572 ± 0.036
3.934 ± 0.025
8.518 ± 0.037
2.086 ± 0.018
5.555 ± 0.025
3.521 ± 0.031
9.866 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.905 ± 0.017
2.988 ± 0.021
4.857 ± 0.026
3.384 ± 0.019
5.992 ± 0.033
5.613 ± 0.024
5.664 ± 0.028
7.219 ± 0.03
1.416 ± 0.016
2.305 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here