Marinobacter litoralis
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3156 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M2RMB6|A0A3M2RMB6_9ALTE Putative HTH-type transcriptional regulator YvdT OS=Marinobacter litoralis OX=187981 GN=yvdT PE=4 SV=1
MM1 pKa = 7.76 TIRR4 pKa = 11.84 KK5 pKa = 9.0 NMLIASIATAGFALAGTASAADD27 pKa = 3.93 MYY29 pKa = 11.05 KK30 pKa = 10.42 SGVGGLYY37 pKa = 10.54 AGFNYY42 pKa = 10.13 TFMNVDD48 pKa = 3.81 FDD50 pKa = 5.27 EE51 pKa = 4.53 INKK54 pKa = 10.07 DD55 pKa = 3.22 ADD57 pKa = 3.38 VGTLSGKK64 pKa = 9.92 VGVMANEE71 pKa = 4.07 YY72 pKa = 10.54 VGLEE76 pKa = 3.74 ARR78 pKa = 11.84 AGFGANDD85 pKa = 3.75 DD86 pKa = 4.89 DD87 pKa = 4.83 IYY89 pKa = 11.54 GVDD92 pKa = 3.66 VEE94 pKa = 4.59 IDD96 pKa = 3.52 NFFGGYY102 pKa = 8.28 ATFNMVNEE110 pKa = 4.56 SPVTPYY116 pKa = 11.12 AVLGFTRR123 pKa = 11.84 AEE125 pKa = 4.05 LEE127 pKa = 4.31 AGSEE131 pKa = 4.17 SDD133 pKa = 4.12 DD134 pKa = 4.43 GSDD137 pKa = 3.41 FSYY140 pKa = 11.04 GAGLNFKK147 pKa = 9.01 MAQNVSGNLEE157 pKa = 3.78 YY158 pKa = 10.08 MRR160 pKa = 11.84 YY161 pKa = 9.92 YY162 pKa = 10.99 DD163 pKa = 5.46 DD164 pKa = 6.5 DD165 pKa = 4.51 DD166 pKa = 4.05 VTVDD170 pKa = 4.46 GIGLGVQFNFF180 pKa = 4.38
Molecular weight: 19.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.732
IPC_protein 3.732
Toseland 3.503
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.859
Patrickios 0.998
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A3M2RCY8|A0A3M2RCY8_9ALTE Phosphate regulon transcriptional regulatory protein PhoB OS=Marinobacter litoralis OX=187981 GN=phoB PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.34 VISRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3156
0
3156
1057142
29
2504
335.0
36.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.724 ± 0.041
0.914 ± 0.015
5.692 ± 0.036
6.721 ± 0.043
3.798 ± 0.028
7.713 ± 0.038
2.198 ± 0.025
5.242 ± 0.031
4.065 ± 0.035
10.603 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.593 ± 0.023
3.454 ± 0.027
4.645 ± 0.027
4.121 ± 0.033
6.077 ± 0.037
5.999 ± 0.027
5.17 ± 0.027
7.354 ± 0.036
1.346 ± 0.017
2.571 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here