Amino acid dipepetide frequency for Microbacterium sp. ABRD_28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.687AlaAla: 21.687 ± 0.215	0.649AlaCys: 0.649 ± 0.028	8.779AlaAsp: 8.779 ± 0.102	8.398AlaGlu: 8.398 ± 0.111	4.079AlaPhe: 4.079 ± 0.077	12.655AlaGly: 12.655 ± 0.147	2.506AlaHis: 2.506 ± 0.053	5.795AlaIle: 5.795 ± 0.083	2.07AlaLys: 2.07 ± 0.057	14.583AlaLeu: 14.583 ± 0.143	2.552AlaMet: 2.552 ± 0.053	1.992AlaAsn: 1.992 ± 0.048	6.919AlaPro: 6.919 ± 0.102	3.615AlaGln: 3.615 ± 0.068	10.363AlaArg: 10.363 ± 0.129	7.014AlaSer: 7.014 ± 0.092	7.488AlaThr: 7.488 ± 0.089	12.239AlaVal: 12.239 ± 0.13	2.033AlaTrp: 2.033 ± 0.052	2.367AlaTyr: 2.367 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.682CysAla: 0.682 ± 0.023	0.047CysCys: 0.047 ± 0.008	0.314CysAsp: 0.314 ± 0.018	0.272CysGlu: 0.272 ± 0.018	0.153CysPhe: 0.153 ± 0.013	0.529CysGly: 0.529 ± 0.023	0.115CysHis: 0.115 ± 0.011	0.169CysIle: 0.169 ± 0.013	0.043CysLys: 0.043 ± 0.006	0.44CysLeu: 0.44 ± 0.023	0.08CysMet: 0.08 ± 0.01	0.101CysAsn: 0.101 ± 0.011	0.269CysPro: 0.269 ± 0.019	0.117CysGln: 0.117 ± 0.012	0.346CysArg: 0.346 ± 0.018	0.268CysSer: 0.268 ± 0.016	0.287CysThr: 0.287 ± 0.015	0.412CysVal: 0.412 ± 0.021	0.068CysTrp: 0.068 ± 0.008	0.101CysTyr: 0.101 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	9.584AspAla: 9.584 ± 0.103	0.221AspCys: 0.221 ± 0.016	5.042AspAsp: 5.042 ± 0.092	4.431AspGlu: 4.431 ± 0.074	1.676AspPhe: 1.676 ± 0.044	6.173AspGly: 6.173 ± 0.091	1.256AspHis: 1.256 ± 0.036	2.62AspIle: 2.62 ± 0.05	0.825AspLys: 0.825 ± 0.031	6.632AspLeu: 6.632 ± 0.084	0.793AspMet: 0.793 ± 0.031	0.898AspAsn: 0.898 ± 0.03	4.64AspPro: 4.64 ± 0.071	1.418AspGln: 1.418 ± 0.038	5.021AspArg: 5.021 ± 0.076	2.407AspSer: 2.407 ± 0.049	3.15AspThr: 3.15 ± 0.063	5.763AspVal: 5.763 ± 0.079	0.863AspTrp: 0.863 ± 0.033	1.229AspTyr: 1.229 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.325GluAla: 7.325 ± 0.099	0.251GluCys: 0.251 ± 0.017	2.853GluAsp: 2.853 ± 0.06	3.248GluGlu: 3.248 ± 0.073	1.691GluPhe: 1.691 ± 0.045	4.44GluGly: 4.44 ± 0.085	1.208GluHis: 1.208 ± 0.038	2.877GluIle: 2.877 ± 0.06	1.168GluLys: 1.168 ± 0.044	6.029GluLeu: 6.029 ± 0.081	0.891GluMet: 0.891 ± 0.029	1.186GluAsn: 1.186 ± 0.035	3.134GluPro: 3.134 ± 0.058	1.964GluGln: 1.964 ± 0.053	5.715GluArg: 5.715 ± 0.076	2.65GluSer: 2.65 ± 0.048	3.157GluThr: 3.157 ± 0.055	4.957GluVal: 4.957 ± 0.075	0.981GluTrp: 0.981 ± 0.027	1.127GluTyr: 1.127 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.39PheAla: 4.39 ± 0.079	0.159PheCys: 0.159 ± 0.013	2.522PheAsp: 2.522 ± 0.052	1.695PheGlu: 1.695 ± 0.047	1.137PhePhe: 1.137 ± 0.046	3.351PheGly: 3.351 ± 0.055	0.554PheHis: 0.554 ± 0.025	1.162PheIle: 1.162 ± 0.039	0.335PheLys: 0.335 ± 0.017	3.127PheLeu: 3.127 ± 0.074	0.399PheMet: 0.399 ± 0.021	0.593PheAsn: 0.593 ± 0.027	1.505PhePro: 1.505 ± 0.038	0.748PheGln: 0.748 ± 0.028	1.941PheArg: 1.941 ± 0.044	1.766PheSer: 1.766 ± 0.046	2.062PheThr: 2.062 ± 0.049	2.905PheVal: 2.905 ± 0.058	0.552PheTrp: 0.552 ± 0.026	0.653PheTyr: 0.653 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.337GlyAla: 11.337 ± 0.146	0.566GlyCys: 0.566 ± 0.023	5.446GlyAsp: 5.446 ± 0.087	5.437GlyGlu: 5.437 ± 0.086	3.352GlyPhe: 3.352 ± 0.062	8.078GlyGly: 8.078 ± 0.15	1.74GlyHis: 1.74 ± 0.037	4.687GlyIle: 4.687 ± 0.069	1.789GlyLys: 1.789 ± 0.05	8.643GlyLeu: 8.643 ± 0.09	2.018GlyMet: 2.018 ± 0.047	1.568GlyAsn: 1.568 ± 0.045	4.069GlyPro: 4.069 ± 0.058	2.325GlyGln: 2.325 ± 0.05	6.765GlyArg: 6.765 ± 0.093	5.099GlySer: 5.099 ± 0.089	5.248GlyThr: 5.248 ± 0.085	8.491GlyVal: 8.491 ± 0.109	1.684GlyTrp: 1.684 ± 0.045	2.077GlyTyr: 2.077 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.469HisAla: 2.469 ± 0.053	0.104HisCys: 0.104 ± 0.011	1.289HisAsp: 1.289 ± 0.044	1.025HisGlu: 1.025 ± 0.033	0.51HisPhe: 0.51 ± 0.025	1.91HisGly: 1.91 ± 0.048	0.566HisHis: 0.566 ± 0.027	0.726HisIle: 0.726 ± 0.029	0.216HisLys: 0.216 ± 0.015	2.055HisLeu: 2.055 ± 0.049	0.274HisMet: 0.274 ± 0.015	0.318HisAsn: 0.318 ± 0.017	1.607HisPro: 1.607 ± 0.044	0.435HisGln: 0.435 ± 0.021	1.648HisArg: 1.648 ± 0.046	0.873HisSer: 0.873 ± 0.029	0.966HisThr: 0.966 ± 0.032	1.678HisVal: 1.678 ± 0.039	0.268HisTrp: 0.268 ± 0.016	0.388HisTyr: 0.388 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.138IleAla: 7.138 ± 0.086	0.211IleCys: 0.211 ± 0.015	3.605IleAsp: 3.605 ± 0.054	2.75IleGlu: 2.75 ± 0.053	1.181IlePhe: 1.181 ± 0.038	4.646IleGly: 4.646 ± 0.075	0.702IleHis: 0.702 ± 0.029	1.72IleIle: 1.72 ± 0.05	0.586IleLys: 0.586 ± 0.028	3.995IleLeu: 3.995 ± 0.068	0.524IleMet: 0.524 ± 0.026	0.791IleAsn: 0.791 ± 0.032	2.483IlePro: 2.483 ± 0.053	1.002IleGln: 1.002 ± 0.033	2.906IleArg: 2.906 ± 0.057	2.234IleSer: 2.234 ± 0.046	2.562IleThr: 2.562 ± 0.06	4.699IleVal: 4.699 ± 0.072	0.505IleTrp: 0.505 ± 0.021	0.759IleTyr: 0.759 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	1.943LysAla: 1.943 ± 0.055	0.051LysCys: 0.051 ± 0.008	0.962LysAsp: 0.962 ± 0.035	0.813LysGlu: 0.813 ± 0.032	0.352LysPhe: 0.352 ± 0.019	1.271LysGly: 1.271 ± 0.04	0.316LysHis: 0.316 ± 0.018	0.757LysIle: 0.757 ± 0.032	0.616LysLys: 0.616 ± 0.032	1.422LysLeu: 1.422 ± 0.043	0.309LysMet: 0.309 ± 0.017	0.391LysAsn: 0.391 ± 0.023	0.923LysPro: 0.923 ± 0.03	0.491LysGln: 0.491 ± 0.023	1.276LysArg: 1.276 ± 0.041	0.833LysSer: 0.833 ± 0.025	1.115LysThr: 1.115 ± 0.038	1.391LysVal: 1.391 ± 0.044	0.196LysTrp: 0.196 ± 0.013	0.342LysTyr: 0.342 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.758LeuAla: 14.758 ± 0.136	0.506LeuCys: 0.506 ± 0.023	6.578LeuAsp: 6.578 ± 0.092	5.065LeuGlu: 5.065 ± 0.078	3.045LeuPhe: 3.045 ± 0.068	9.22LeuGly: 9.22 ± 0.105	1.809LeuHis: 1.809 ± 0.042	4.595LeuIle: 4.595 ± 0.088	1.42LeuLys: 1.42 ± 0.045	10.109LeuLeu: 10.109 ± 0.139	1.557LeuMet: 1.557 ± 0.044	1.6LeuAsn: 1.6 ± 0.044	5.683LeuPro: 5.683 ± 0.08	2.535LeuGln: 2.535 ± 0.059	7.977LeuArg: 7.977 ± 0.102	5.82LeuSer: 5.82 ± 0.075	6.304LeuThr: 6.304 ± 0.07	9.271LeuVal: 9.271 ± 0.116	1.354LeuTrp: 1.354 ± 0.038	1.601LeuTyr: 1.601 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.017MetAla: 2.017 ± 0.047	0.095MetCys: 0.095 ± 0.01	0.773MetAsp: 0.773 ± 0.028	0.585MetGlu: 0.585 ± 0.026	0.465MetPhe: 0.465 ± 0.02	1.24MetGly: 1.24 ± 0.039	0.308MetHis: 0.308 ± 0.017	0.808MetIle: 0.808 ± 0.032	0.353MetLys: 0.353 ± 0.021	1.761MetLeu: 1.761 ± 0.042	0.32MetMet: 0.32 ± 0.018	0.456MetAsn: 0.456 ± 0.024	1.123MetPro: 1.123 ± 0.032	0.492MetGln: 0.492 ± 0.022	1.412MetArg: 1.412 ± 0.037	1.353MetSer: 1.353 ± 0.036	1.744MetThr: 1.744 ± 0.041	1.29MetVal: 1.29 ± 0.036	0.216MetTrp: 0.216 ± 0.017	0.27MetTyr: 0.27 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.256AsnAla: 2.256 ± 0.05	0.101AsnCys: 0.101 ± 0.011	1.092AsnAsp: 1.092 ± 0.031	0.87AsnGlu: 0.87 ± 0.031	0.577AsnPhe: 0.577 ± 0.026	1.633AsnGly: 1.633 ± 0.054	0.326AsnHis: 0.326 ± 0.018	0.769AsnIle: 0.769 ± 0.031	0.273AsnLys: 0.273 ± 0.017	1.738AsnLeu: 1.738 ± 0.045	0.25AsnMet: 0.25 ± 0.016	0.367AsnAsn: 0.367 ± 0.021	1.553AsnPro: 1.553 ± 0.049	0.434AsnGln: 0.434 ± 0.023	1.21AsnArg: 1.21 ± 0.034	0.782AsnSer: 0.782 ± 0.029	0.973AsnThr: 0.973 ± 0.034	1.485AsnVal: 1.485 ± 0.043	0.25AsnTrp: 0.25 ± 0.015	0.393AsnTyr: 0.393 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.779ProAla: 7.779 ± 0.109	0.209ProCys: 0.209 ± 0.014	4.253ProAsp: 4.253 ± 0.069	3.859ProGlu: 3.859 ± 0.072	1.831ProPhe: 1.831 ± 0.048	5.411ProGly: 5.411 ± 0.097	1.17ProHis: 1.17 ± 0.039	2.086ProIle: 2.086 ± 0.044	0.854ProLys: 0.854 ± 0.033	5.18ProLeu: 5.18 ± 0.067	0.81ProMet: 0.81 ± 0.03	0.924ProAsn: 0.924 ± 0.03	2.609ProPro: 2.609 ± 0.067	1.454ProGln: 1.454 ± 0.04	3.92ProArg: 3.92 ± 0.058	3.185ProSer: 3.185 ± 0.065	3.556ProThr: 3.556 ± 0.072	5.071ProVal: 5.071 ± 0.075	0.921ProTrp: 0.921 ± 0.033	1.016ProTyr: 1.016 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.267GlnAla: 3.267 ± 0.066	0.085GlnCys: 0.085 ± 0.009	1.179GlnAsp: 1.179 ± 0.035	1.253GlnGlu: 1.253 ± 0.031	0.835GlnPhe: 0.835 ± 0.031	1.961GlnGly: 1.961 ± 0.048	0.534GlnHis: 0.534 ± 0.025	1.338GlnIle: 1.338 ± 0.037	0.526GlnLys: 0.526 ± 0.027	2.944GlnLeu: 2.944 ± 0.066	0.467GlnMet: 0.467 ± 0.023	0.577GlnAsn: 0.577 ± 0.027	1.421GlnPro: 1.421 ± 0.043	0.964GlnGln: 0.964 ± 0.035	2.351GlnArg: 2.351 ± 0.046	1.4GlnSer: 1.4 ± 0.037	1.579GlnThr: 1.579 ± 0.043	2.244GlnVal: 2.244 ± 0.046	0.445GlnTrp: 0.445 ± 0.021	0.555GlnTyr: 0.555 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.914ArgAla: 9.914 ± 0.103	0.319ArgCys: 0.319 ± 0.02	4.743ArgAsp: 4.743 ± 0.078	4.659ArgGlu: 4.659 ± 0.076	2.599ArgPhe: 2.599 ± 0.054	6.131ArgGly: 6.131 ± 0.077	1.829ArgHis: 1.829 ± 0.04	3.745ArgIle: 3.745 ± 0.065	1.177ArgLys: 1.177 ± 0.04	7.698ArgLeu: 7.698 ± 0.096	1.878ArgMet: 1.878 ± 0.044	1.234ArgAsn: 1.234 ± 0.037	4.196ArgPro: 4.196 ± 0.082	2.075ArgGln: 2.075 ± 0.047	7.786ArgArg: 7.786 ± 0.108	4.17ArgSer: 4.17 ± 0.075	4.448ArgThr: 4.448 ± 0.078	6.795ArgVal: 6.795 ± 0.09	1.274ArgTrp: 1.274 ± 0.039	1.536ArgTyr: 1.536 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.955SerAla: 6.955 ± 0.09	0.244SerCys: 0.244 ± 0.015	3.252SerAsp: 3.252 ± 0.06	2.733SerGlu: 2.733 ± 0.046	1.837SerPhe: 1.837 ± 0.046	5.475SerGly: 5.475 ± 0.078	0.994SerHis: 0.994 ± 0.032	2.277SerIle: 2.277 ± 0.048	0.746SerLys: 0.746 ± 0.025	5.213SerLeu: 5.213 ± 0.079	1.015SerMet: 1.015 ± 0.031	0.85SerAsn: 0.85 ± 0.03	3.234SerPro: 3.234 ± 0.065	1.285SerGln: 1.285 ± 0.035	3.925SerArg: 3.925 ± 0.067	3.296SerSer: 3.296 ± 0.076	3.299SerThr: 3.299 ± 0.056	4.608SerVal: 4.608 ± 0.074	0.93SerTrp: 0.93 ± 0.034	1.075SerTyr: 1.075 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	7.923ThrAla: 7.923 ± 0.101	0.274ThrCys: 0.274 ± 0.017	3.64ThrAsp: 3.64 ± 0.067	3.037ThrGlu: 3.037 ± 0.058	1.866ThrPhe: 1.866 ± 0.045	5.888ThrGly: 5.888 ± 0.082	1.094ThrHis: 1.094 ± 0.037	2.643ThrIle: 2.643 ± 0.052	0.883ThrLys: 0.883 ± 0.034	6.124ThrLeu: 6.124 ± 0.072	0.954ThrMet: 0.954 ± 0.035	0.953ThrAsn: 0.953 ± 0.036	4.132ThrPro: 4.132 ± 0.083	1.389ThrGln: 1.389 ± 0.04	4.03ThrArg: 4.03 ± 0.074	3.198ThrSer: 3.198 ± 0.063	3.64ThrThr: 3.64 ± 0.061	5.857ThrVal: 5.857 ± 0.083	0.935ThrTrp: 0.935 ± 0.034	1.135ThrTyr: 1.135 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	12.335ValAla: 12.335 ± 0.13	0.499ValCys: 0.499 ± 0.026	6.145ValAsp: 6.145 ± 0.083	4.948ValGlu: 4.948 ± 0.072	3.096ValPhe: 3.096 ± 0.066	7.388ValGly: 7.388 ± 0.088	1.674ValHis: 1.674 ± 0.042	4.788ValIle: 4.788 ± 0.072	1.357ValLys: 1.357 ± 0.037	9.292ValLeu: 9.292 ± 0.107	1.438ValMet: 1.438 ± 0.036	1.712ValAsn: 1.712 ± 0.043	4.838ValPro: 4.838 ± 0.074	2.108ValGln: 2.108 ± 0.047	6.521ValArg: 6.521 ± 0.09	5.064ValSer: 5.064 ± 0.068	6.008ValThr: 6.008 ± 0.083	9.293ValVal: 9.293 ± 0.135	1.218ValTrp: 1.218 ± 0.036	1.536ValTyr: 1.536 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.648TrpAla: 1.648 ± 0.04	0.11TrpCys: 0.11 ± 0.009	0.861TrpAsp: 0.861 ± 0.031	0.699TrpGlu: 0.699 ± 0.026	0.612TrpPhe: 0.612 ± 0.024	1.154TrpGly: 1.154 ± 0.031	0.331TrpHis: 0.331 ± 0.02	0.737TrpIle: 0.737 ± 0.028	0.265TrpLys: 0.265 ± 0.017	1.721TrpLeu: 1.721 ± 0.046	0.321TrpMet: 0.321 ± 0.016	0.415TrpAsn: 0.415 ± 0.022	0.772TrpPro: 0.772 ± 0.031	0.568TrpGln: 0.568 ± 0.023	1.357TrpArg: 1.357 ± 0.042	0.901TrpSer: 0.901 ± 0.027	0.936TrpThr: 0.936 ± 0.033	1.258TrpVal: 1.258 ± 0.041	0.402TrpTrp: 0.402 ± 0.023	0.309TrpTyr: 0.309 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.373TyrAla: 2.373 ± 0.05	0.113TyrCys: 0.113 ± 0.011	1.317TyrAsp: 1.317 ± 0.036	1.078TyrGlu: 1.078 ± 0.037	0.662TyrPhe: 0.662 ± 0.025	1.862TyrGly: 1.862 ± 0.043	0.297TyrHis: 0.297 ± 0.019	0.698TyrIle: 0.698 ± 0.027	0.265TyrLys: 0.265 ± 0.016	2.096TyrLeu: 2.096 ± 0.051	0.229TyrMet: 0.229 ± 0.014	0.408TyrAsn: 0.408 ± 0.022	0.989TyrPro: 0.989 ± 0.032	0.507TyrGln: 0.507 ± 0.022	1.74TyrArg: 1.74 ± 0.043	0.93TyrSer: 0.93 ± 0.031	1.074TyrThr: 1.074 ± 0.032	1.55TyrVal: 1.55 ± 0.038	0.281TyrTrp: 0.281 ± 0.017	0.441TyrTyr: 0.441 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3036 proteins (994539 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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