Streptococcus phage Javan182

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AUN4|A0A4D6AUN4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan182 OX=2548030 GN=Javan182_0046 PE=4 SV=1
MM1 pKa = 6.96MVKK4 pKa = 10.16EE5 pKa = 4.86LIEE8 pKa = 4.21KK9 pKa = 9.17LQEE12 pKa = 4.02YY13 pKa = 9.87PDD15 pKa = 4.06YY16 pKa = 10.68LTVEE20 pKa = 4.47LVLEE24 pKa = 4.51DD25 pKa = 3.51VDD27 pKa = 4.59LVEE30 pKa = 5.41AAHH33 pKa = 6.41EE34 pKa = 4.13VDD36 pKa = 4.66NVLSANIITYY46 pKa = 8.96DD47 pKa = 3.48EE48 pKa = 4.2KK49 pKa = 10.7TLGVEE54 pKa = 3.97QVSIDD59 pKa = 3.71KK60 pKa = 10.94LIISATGCC68 pKa = 3.43

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATD2|A0A4D6ATD2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan182 OX=2548030 GN=Javan182_0029 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.6FKK5 pKa = 10.95FKK7 pKa = 11.06LNPAGVAEE15 pKa = 4.26LMKK18 pKa = 10.42SEE20 pKa = 4.54EE21 pKa = 4.14MQQVLTTKK29 pKa = 9.48ATAIRR34 pKa = 11.84EE35 pKa = 4.25RR36 pKa = 11.84CGDD39 pKa = 3.82GYY41 pKa = 9.01TQDD44 pKa = 2.62IHH46 pKa = 7.83VGKK49 pKa = 10.1NRR51 pKa = 11.84ANAMVSAKK59 pKa = 9.54TIKK62 pKa = 10.38AKK64 pKa = 10.43KK65 pKa = 10.15DD66 pKa = 3.23NSKK69 pKa = 11.36NNTLLKK75 pKa = 10.41AVRR78 pKa = 3.79

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10170

43

786

207.6

23.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.316 ± 0.676

0.718 ± 0.13

6.441 ± 0.257

6.853 ± 0.492

3.756 ± 0.203

6.952 ± 0.536

1.495 ± 0.158

7.384 ± 0.327

8.604 ± 0.357

8.604 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.173

5.3 ± 0.299

2.891 ± 0.329

3.953 ± 0.216

3.845 ± 0.273

6.608 ± 0.375

5.752 ± 0.355

6.45 ± 0.358

1.062 ± 0.145

3.884 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski