Mycobacterium phage Cooper

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Coopervirus; Mycobacterium virus Cooper

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1A020|Q1A020_9CAUD Uncharacterized protein OS=Mycobacterium phage Cooper OX=373406 GN=96 PE=4 SV=1
MM1 pKa = 7.61YY2 pKa = 10.27DD3 pKa = 3.37PANVVEE9 pKa = 5.07PPSCDD14 pKa = 2.85SCAGYY19 pKa = 8.35ITDD22 pKa = 5.34HH23 pKa = 6.71EE24 pKa = 4.52PACPVGQGITSTLEE38 pKa = 3.38NHH40 pKa = 6.99LAGEE44 pKa = 4.37DD45 pKa = 3.94AQWALASRR53 pKa = 11.84IVEE56 pKa = 4.04WEE58 pKa = 3.85LTEE61 pKa = 4.2NAAYY65 pKa = 9.34WRR67 pKa = 11.84DD68 pKa = 3.36RR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.7ARR73 pKa = 11.84AA74 pKa = 3.3

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1A043|Q1A043_9CAUD Uncharacterized protein OS=Mycobacterium phage Cooper OX=373406 GN=73 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 9.97ARR5 pKa = 11.84KK6 pKa = 8.77GSRR9 pKa = 11.84RR10 pKa = 11.84GGALRR15 pKa = 11.84GGRR18 pKa = 11.84SKK20 pKa = 10.51IARR23 pKa = 11.84SRR25 pKa = 11.84GRR27 pKa = 11.84GTVALHH33 pKa = 6.38AKK35 pKa = 10.06AGGIATFAKK44 pKa = 10.38GGGGGYY50 pKa = 10.11RR51 pKa = 11.84GFKK54 pKa = 10.23SKK56 pKa = 10.67KK57 pKa = 5.54QWRR60 pKa = 11.84WAWATHH66 pKa = 5.45QPWARR71 pKa = 11.84KK72 pKa = 9.22KK73 pKa = 9.3SHH75 pKa = 5.63EE76 pKa = 4.29TAGGPKK82 pKa = 9.39VRR84 pKa = 11.84YY85 pKa = 9.27RR86 pKa = 11.84RR87 pKa = 11.84LPASKK92 pKa = 10.38HH93 pKa = 5.76SGHH96 pKa = 6.59KK97 pKa = 10.2GSRR100 pKa = 11.84APKK103 pKa = 9.51SS104 pKa = 3.38

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

22614

48

2023

228.4

24.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.775 ± 0.431

1.061 ± 0.143

6.633 ± 0.31

5.386 ± 0.305

2.454 ± 0.106

9.733 ± 0.679

1.955 ± 0.161

3.922 ± 0.213

2.932 ± 0.159

8.19 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.101

2.905 ± 0.176

6.288 ± 0.225

3.626 ± 0.133

6.266 ± 0.31

4.838 ± 0.196

6.894 ± 0.208

7.694 ± 0.245

1.977 ± 0.146

2.299 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski