Bacillus virus 250

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cecivirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2XPZ9|D2XPZ9_9CAUD Holin OS=Bacillus virus 250 OX=701257 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 10.38YY4 pKa = 10.48YY5 pKa = 10.3GYY7 pKa = 10.85CYY9 pKa = 10.16DD10 pKa = 3.9ANGKK14 pKa = 7.54FTEE17 pKa = 5.22IIPLDD22 pKa = 3.77EE23 pKa = 4.39KK24 pKa = 10.89PIYY27 pKa = 9.79EE28 pKa = 4.07KK29 pKa = 9.99QTFHH33 pKa = 8.36RR34 pKa = 11.84EE35 pKa = 3.8EE36 pKa = 3.77QKK38 pKa = 11.06EE39 pKa = 4.1IITEE43 pKa = 4.0EE44 pKa = 4.29KK45 pKa = 10.26LCEE48 pKa = 3.87VHH50 pKa = 7.03QSIEE54 pKa = 4.2DD55 pKa = 3.69GTYY58 pKa = 10.28EE59 pKa = 4.91PPLPDD64 pKa = 4.56PGEE67 pKa = 4.23GPEE70 pKa = 4.8NIDD73 pKa = 3.21EE74 pKa = 5.23AIVFEE79 pKa = 4.35ATYY82 pKa = 10.5SEE84 pKa = 5.29PISKK88 pKa = 9.68QDD90 pKa = 3.6CPDD93 pKa = 3.38CVMHH97 pKa = 6.06NVEE100 pKa = 4.29YY101 pKa = 9.13EE102 pKa = 4.37TIKK105 pKa = 10.79VLYY108 pKa = 9.44EE109 pKa = 3.54EE110 pKa = 4.8DD111 pKa = 3.87VIVGYY116 pKa = 10.71EE117 pKa = 3.65PDD119 pKa = 3.38IPANCTLEE127 pKa = 4.08VCPWLAYY134 pKa = 10.63DD135 pKa = 4.29PVFDD139 pKa = 3.88GEE141 pKa = 4.44KK142 pKa = 8.93WVKK145 pKa = 8.8TVEE148 pKa = 4.17PQPEE152 pKa = 4.27EE153 pKa = 4.02PQPEE157 pKa = 4.29EE158 pKa = 3.68PSEE161 pKa = 4.03LEE163 pKa = 3.99KK164 pKa = 11.03LKK166 pKa = 10.4QHH168 pKa = 6.2QEE170 pKa = 3.69LMQQALDD177 pKa = 3.82EE178 pKa = 4.6LLLGGMM184 pKa = 4.85

Molecular weight: 21.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2XPY4|D2XPY4_9CAUD Uncharacterized protein OS=Bacillus virus 250 OX=701257 PE=4 SV=1
MM1 pKa = 7.28SGKK4 pKa = 9.78SKK6 pKa = 10.5KK7 pKa = 9.71YY8 pKa = 8.95PAWSTTSYY16 pKa = 11.17GDD18 pKa = 3.11AAGILGVNCRR28 pKa = 11.84HH29 pKa = 6.18IKK31 pKa = 9.92YY32 pKa = 9.54PYY34 pKa = 9.09IPGMSTKK41 pKa = 10.32RR42 pKa = 11.84NEE44 pKa = 4.16PQDD47 pKa = 3.54YY48 pKa = 10.97AEE50 pKa = 4.25NNRR53 pKa = 11.84VYY55 pKa = 10.66KK56 pKa = 10.43EE57 pKa = 4.07SQRR60 pKa = 11.84QRR62 pKa = 11.84SLEE65 pKa = 3.9RR66 pKa = 11.84DD67 pKa = 2.82IKK69 pKa = 9.26KK70 pKa = 10.54AKK72 pKa = 9.96RR73 pKa = 11.84EE74 pKa = 3.9VMLLEE79 pKa = 4.05EE80 pKa = 5.23LGDD83 pKa = 3.79KK84 pKa = 10.93EE85 pKa = 4.43GVKK88 pKa = 9.92LAKK91 pKa = 10.14QKK93 pKa = 10.27VSQRR97 pKa = 11.84QAAMRR102 pKa = 11.84DD103 pKa = 3.99FIKK106 pKa = 10.28ATGRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84PEE115 pKa = 3.45RR116 pKa = 11.84EE117 pKa = 3.61KK118 pKa = 10.54IYY120 pKa = 10.11TVV122 pKa = 3.11

Molecular weight: 14.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13254

78

1235

245.4

27.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.503

0.8 ± 0.129

5.515 ± 0.253

7.696 ± 0.566

3.901 ± 0.145

6.006 ± 0.343

1.773 ± 0.165

7.137 ± 0.31

8.926 ± 0.543

7.99 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.131 ± 0.276

5.96 ± 0.253

3.199 ± 0.204

4.014 ± 0.235

4.61 ± 0.29

5.636 ± 0.28

6.028 ± 0.289

6.006 ± 0.272

1.147 ± 0.098

3.946 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski